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当前位置: 首页 > 产品中心 > peptide > Qiagen/PAXgene Blood ccfDNA Tubes (100)/100 blood collection tubes (10 ml). To be used in conjunction with the QIAamp MinElute ccfDNA Kit, the QIAamp Circulating Nucleic Acid Kit (50) or the QIAsymphony PAXgene Blood ccfDNA Kit (192)/768115
商品详细Qiagen/PAXgene Blood ccfDNA Tubes (100)/100 blood collection tubes (10 ml). To be used in conjunction with the QIAamp MinElute ccfDNA Kit, the QIAamp Circulating Nucleic Acid Kit (50) or the QIAsymphony PAXgene Blood ccfDNA Kit (192)/768115
Qiagen/PAXgene Blood ccfDNA Tubes (100)/100 blood collection tubes (10 ml). To be used in conjunction with the QIAamp MinElute ccfDNA Kit, the QIAamp Circulating Nucleic Acid Kit (50) or the QIAsymphony PAXgene Blood ccfDNA Kit (192)/768115
Qiagen/PAXgene Blood ccfDNA Tubes (100)/100 blood collection tubes (10 ml). To be used in conjunction with the QIAamp MinElute ccfDNA Kit, the QIAamp Circulating Nucleic Acid Kit (50) or the QIAsymphony PAXgene Blood ccfDNA Kit (192)/768115
商品编号: 768115
品牌: Qiagen
市场价: ¥17120.00
美元价: 10272.00
产地: 美国(厂家直采)
公司:
产品分类: 多肽合成
公司分类: peptide
联系Q Q: 3392242852
电话号码: 4000-520-616
电子邮箱: info@ebiomall.com
商品介绍

PAXgene Blood ccfDNA Tubes (100)

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For collection of whole blood samplesand stabilization of ccfDNA

  • Single-tube collection, stabilization, transport and storage
  • Immediate stabilization of circulating, cell-free DNA (ccfDNA)
  • Sample transport and storage at room temperature for up to 10 days
  • Standardized preanalytical processing of samples
The PAXgene Blood ccfDNA Tube is a plastic, closed, evacuated tube for the collection, anticoagulation, transport and storageof human whole blood samples and the stabilization of ccfDNA. When the tube is used in conjunction with theQIAsymphony PAXgene Blood ccfDNA Kit, the QIAGENQIAamp MinElute ccfDNA Kit or the QIAamp Circulating Nucleic Acid Kit, high-quality ccfDNA can be isolated for use in downstream analytical assays.
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Cat No./ID:768115
PAXgene Blood ccfDNA Tubes (100)
$856.00
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100 blood collection tubes (10 ml). To be used in conjunction with the QIAamp MinElute ccfDNA Kit, theQIAamp Circulating Nucleic Acid Kit (50) or the QIAsymphony PAXgene Blood ccfDNA Kit (192)
For Research Use Only. Not for use in diagnostics procedures. No claim or representation is intended to provide information for the diagnosis, prevention, or treatment of a disease.

Product Details

9
PAXgene Blood ccfDNA stabilization helps prevent apoptosis and fragmentation of gDNA in whole blood.

Whole blood from 2 subjects was stored in PAXgene Blood ccfDNA Tubes and EDTA tubes. Genomic DNA was extracted from samples using the QIAamp Blood Mini Kit immediately after blood collection and after 10 days of storage. Agarose gel electrophoresis was used for size separation of 400 ng extracted gDNA. Fragmentation was evident in the gDNA extracted from whole blood stored in EDTA tubes, but practically absent from the samples stored in PAXgene Blood ccfDNA Tubes.

9
PAXgene Blood ccfDNA stabilization minimizes hemolysis compared to alternative solutions.

Whole blood from 20 subjects was collected into PAXgene Blood ccfDNA Tubes, EDTA tubes and blood collection tubes designed for ccfDNA stabilizationfrom 2 other suppliers. Plasma was separated directly after blood draw, after 3 days and after 7 days storage at room temperature (15–25°C). Hemolysis wasassessed by measuring plasma free hemoglobin (pfHb)using the Catachem Plasma Free Hemoglobin (PFH) Kit on the Beckman Counter DxC analyzer. Increase in sample hemolysis during sample storage at room temperature wasminimized in the PAXgene Blood ccfDNA Tubescompared to the other blood collection tubes with different stabilization solutions. This indicates red blood cell lysis and decreased sample integrity in the alternative blood collection tubes.

9
PAXgene Blood ccfDNA stabilization helps prevent release of gDNA into plasma during storage and transport.

Whole blood from 8 subjects wascollected into EDTA tubes (unstabilized) and PAXgene Blood ccfDNA Tubes (stabilized). Samples were stored for 1 day at 35°C (t1, 35°C), for 6 days at room temperature (t6) or to simulate transport, continually inverted for 5 hours and then stored horizontally for 6 days at room temperature (t6sim). ccfDNA was extracted from plasma using the QIAsymphony SP Protocol for the PAXgene Blood ccfDNA System and ccfDNA yield was quantified by real-time PCR (18S rDNA gene, 66 bp, 180 bp and 500 bp amplicons). Shown is the average ratio of copy numbers at testing time (tx) to copy numbers immediately after blood draw (t0), plus standard deviation. The ratio remained close to 1 in all treatments of the PAXgene Blood ccfDNA stabilized samples, indicating the 18S rDNA copy number did not change over time. A significant increase in 18S rDNA copy number was observed in the EDTA samples after storage, indicating release of gDNA into the plasma.

9
PAXgene Blood ccfDNA stabilization reagent helps prevent hemolysis, allowing clear distinction between plasma and cell fractions.

Whole blood was stored in EDTA tubes or PAXgene Blood ccfDNA Tubes. Hemolysis was determined by measuring the absorption of free hemoglobin in plasma from 6 subjects at 414 nm. Mean absorption and standard deviation are shown. Amount of hemolysis increased with each additional storage time point in the EDTA tubes, but remained relatively constant in the PAXgene Blood ccfDNA additive.

9
PAXgene Blood ccfDNA stabilization reagent helps prevent release of gDNA into plasma.

Whole blood was stored in EDTA tubes or PAXgene Blood ccfDNA Tubes. ccfDNA was extracted from the plasma immediately following blood collection (t0) or after 6 days storage (t6) at room temperature. Eluate (1 µl) was analyzed using the Agilent High Sensitivity DNA Kit. Plasma from EDTA tubes showed an increase in apoptotic gDNA fragments, whereas plasma from PAXgene Blood ccfDNA Tubes showed a ccfDNA profile comparable to day 0.

Performance

PAXgene Blood ccfDNA Tubes simplify the collection and processing of whole blood for subsequent purification of circulating, cell-free DNA (ccfDNA). The tube additive stabilizes blood cells (see "PAXgene Blood ccfDNAstabilization helps prevent hemolysis allowing distinctseparation of plasma" and"PAXgene Blood ccfDNA stabilizationminimizes hemolysis compared toalternativesolutions")and blocks apoptosis (see "PAXgene Blood ccfDNA stabilization helps prevent apoptosis and fragmentation of gDNA").Consequently,using PAXgene Blood ccfDNA Tubes forwhole blood collection, storage and transporthelps preventthe release of intracellular DNA into plasma (see "PAXgene Blood ccfDNA stabilization helps prevent release of gDNA into plasma"). ccfDNA levels are stable for up to 10 days at temperatures up to 25°C, 7 days at temperatures up to 30°C, or 3 days at temperatures up to 37°C prior to processing, so blood samples can be transported and stored to process plasma later.Plasma generated from whole blood collected into PAXgene Blood ccfDNA Tubes and processed with theQIAsymphony PAXgene Blood ccfDNA Kit, the QIAGENQIAamp MinElute ccfDNA Kit or the QIAamp Circulating Nucleic Acid Kit, yields high-quality ccfDNA for downstream analytical assays, including but not limited to PCR-based assays and next-generation sequencing (NGS).

Principle
The PAXgene Blood ccfDNA Tube is a plastic, closed, evacuated tube for the collection, anticoagulation, transport and storage of human whole blood samples and the stabilization of ccfDNA. The tube contains an additive that anticoagulates blood and stabilizes blood cells via a non-crosslinking stabilization solution. This helps prevent the release of intracellular DNA into the plasma and maintains constant ccfDNA levels during sample storage before processing.
Procedure
The PAXgene Blood ccfDNA Tube allows collection of 10 ml whole blood into a closed, evacuated system. Using standard venipuncture technique, blood is collected into an evacuated tube that contains 1.5 ml cell stabilization additive, resulting in a ratio of 0.15 ml additive per 1 ml blood. Isolation of ccfDNA from plasma is carried out using theQIAsymphony PAXgene Blood ccfDNA Kit, theQIAampMinElute ccfDNA Kit orthe QIAamp Circulating Nucleic Acid Kit.
Applications

ccfDNA purified from plasma generated from whole blood collected into PAXgene Blood ccfDNA Tubes can be used in a wide range of downstream applications, including:

  • PCR, including digital, multiplex and quantitative real-time PCR
  • Pharmacogenomic studies
  • SNP genotyping
  • Next-generation sequencing

    Specifications

    Features
    Specifications
    Additive/concentrationAdditive 1.5 ml
    Closure type/colorPearl white BD Hemogard/Blue stopper
    Draw volume10 ml
    Label typePaper
    Quantity100 tubes/case
    StabilizationUp to 10 days at temperatures up to 25°C, 7 days at temperatures up to 30°C, or 3 days at temperatures up to 37°C. Note: Do not store blood-filled tubes below 2°C.
    Tube size16 x 100 mm

    Product Resources

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    Brochures & Guides (2)
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    PAXgene Blood ccfDNA System Brochure
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    PAXgene Blood ccfDNA System Transport Concept
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    FAQs (31)
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    Do I need to use a blood collection set with the PAXgene Blood ccfDNA Tube?
    FAQ ID - 3626
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    What is the shelf life of unused PAXgene Blood ccfDNA Tubes?
    FAQ ID - 3633
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    What is the expected ccfDNA yield from plasma generated from 10 ml of blood?
    FAQ ID - 3637
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    Can I use plasma from underfilled PAXgene Blood ccfDNA Tubes?
    FAQ ID - 3638
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    How long can whole blood stabilized in PAXgene Blood ccfDNA Tubes be stored at room temperature?
    FAQ ID - 3639
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    Can whole blood in PAXgene Blood ccfDNA Tubes be stored at temperatures above 25°C?
    FAQ ID - 3640
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    Can whole blood in PAXgene Blood ccfDNA Tubes be stored at temperatures below 15°C?
    FAQ ID - 3641
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    Can whole blood be frozen in PAXgene Blood ccfDNA Tubes?
    FAQ ID - 3642
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    Is double centrifugation required for plasma processing?
    FAQ ID - 3643
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    How long and at which temperature can plasma generated from whole blood in PAXgene Blood ccfDNA Tubes be stored?
    FAQ ID - 3644
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    How should I thaw frozen plasma samples?
    FAQ ID - 3645
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    What should I do if cryoprecipitates form while thawing a frozen plasma sample?
    FAQ ID - 3646
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    Which plasma volumes can I process with the QIAsymphony PAXgene Blood ccfDNA protocols?
    FAQ ID - 3648
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    How many extractions can I perform with a QIAsymphony PAXgene Blood ccfDNA Kit, using the 2.4 ml or the 4.8 ml protocols?
    FAQ ID - 3649
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    How long after first use can a reagent cartridge be stored for further use?
    FAQ ID - 3650
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    What is the shelf life of the QIAsymphony PAXgene Blood ccfDNA Kit?
    FAQ ID - 3651
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    For what type of sample material is the QIAsymphony PAXgene Blood ccfDNA Kit intended?
    FAQ ID - 3652
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    Which tubes are recommended for the QIAsymphony sample rack
    FAQ ID - 3653
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    Which protocol line should I useto process plasma samples?
    FAQ ID - 3654
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    Which elution volumes are available with the QIAsymphony PAXgene Blood ccfDNA Kit?
    FAQ ID - 3655
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    Does the QIAsymphony PAXgene Blood ccfDNA Kit also allow eluting ccfDNA in less than 60 µl?
    FAQ ID - 3656
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    How should eluates produced with the PAXgene Blood ccfDNA System be stored?
    FAQ ID - 3657
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    Which downstream applications have been tested with ccfDNA purified from whole blood collected and processed with the PAXgene Blood ccfDNA System?
    FAQ ID - 3658
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    Why is a blood collection set required to collect blood into the PAXgene Blood ccfDNA Tube?
    FAQ ID - 3628
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    Can reagent from the PAXgene Blood ccfDNA Tube flow back into the patient’s arm?
    FAQ ID - 3629
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    Can I use a different brand blood collection set with the PAXgene Blood ccfDNA Tube?
    FAQ ID - 3630
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    What is the blood draw volume of the PAXgene Blood ccfDNA Tube
    FAQ ID - 3631
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    What is the reagent in the PAXgene Blood ccfDNA Tube?
    FAQ ID - 3632
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    How should unused PAXgene Blood ccfDNA Tubes be stored?
    FAQ ID - 3634
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    Are there special considerations for phlebotomy when using the PAXgene Blood ccfDNA Tube (blood collection set, discard tube, positioning of the tube, issues with short draws, etc.)?
    FAQ ID - 3635
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    Are PAXgene Blood ccfDNA Tubes sterile?
    FAQ ID - 3636
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    Scientific Posters (10)
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    A New Blood Collection Tube and Automated Extraction Method for Stabilization and Analysis of ccfDNA
    Ullius et al., 2015

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    A Shortened, Automated Workflow for Processing Plasma Directly From Primary Blood Collection Tubes Reduces Turnaround Time and Risk of Sample Mix-up in ccfDNA Based Applications
    Vosset al., 2017

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    Integrated Workflow for Blood Collection, ccfDNA Stabilization and Extraction for Non-invasive Prenatal Testing
    Ullius et al., 2016

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    Cell-free DNA Quality and Quantity Assessment – A Method Comparison
    Ullius et al.2019

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    Comparison of Hemolysis in Circulating Cell-free DNA Stabilization Tubes
    Hallemariam et al.2019

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    EGFR Mutation Analysis – Performance Evaluation of Blood Collection Tubes for ccfDNA Stabilization
    Ullius et al.2019

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    Multimodal Analysis of Circulating Tumor Cell RNA, Circulating Cell-Free DNA and Genomic DNA from a Single Blood Sample Collected into the PAXgene Blood ccfDNA Tube
    Babayan et al.2019

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    Performance Evaluation of a New Integrated Blood Collection and Sample Preparation System for the Stabilization and Extraction of Circulating Cell-free DNA (ccfDNA)
    Ullius et al.

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    Precision and Robustness of the PAXgene Blood ccfDNA Workflow
    Krenzet al.2019

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    Quality Assessment of Blood Collection Tubes for ccfDNA at the End of Their Shelf Life
    Ullius et al.2019

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    Supplementary Protocols (1)
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    Purification of genomic DNA from the PAXgene® Blood ccfDNA Tube using the QIAamp® DNA Blood Mini Kit - (EN)
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    Application Notes (2)
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    PAXgene Blood ccfDNA System Transport Concept

    IATA-conform transport of whole blood samples in PAXgene Blood ccfDNA Tubes – a simulation and functional test

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    Multimodal analysis of CTC RNA, ccfDNA and gDNA from a single blood sample collected into a PAXgene Blood ccfDNA Tube (RUO)

    Demonstrated 3-in-1 workflow for the simultaneous extraction of circulating tumor cell RNA, ccfDNA and leukocyte-derived gDNA from a single blood sample collected and stored in a PAXgene Blood ccfDNA Tube (RUO)

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    Kit Handbooks (1)
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    PAXgene Blood ccfDNA Tube (RUO) Handbook
    PAXgene Blood ccfDNA Tube (RUO) Handbook
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    Safety Data Sheets (1)
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    MSDS PAXgene Blood ccfDNA Tubes (100)
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    品牌介绍
    QIAGEN是一家专业化致力于生物分子样品制备解决方案的跨国经营企业,总部位于德国。1984年,QIAGEN在德国成立,1996年在美国纽约纳斯达克上市。QIAGEN拥有超过1000项专利和认可证明,在18个国家设立了分公司,代理商服务国超过40个,在全球有超过400000的用户。QIAGEN提供的产品超过500类,包括各种试剂,耗材和自动化纯化工作站。这些产品用于样品采集,稳定,核酸或蛋白的分离,纯化和检测中,不仅广泛的应用于科研领域的各个方面,在生物技术,制药,法医研究,食品安全检测,畜牧业和分子诊断领域也得到了广泛的应用。QIAGEN产品的卓越品质和在应用中的出色表现使得其成为样品处理中标准的代名词。