Qiagen/PAXgene Tissue STABILIZER/8 bottles of PAXgene Tissue Stabilizer concentrate, for 4 liters of PAXgene Tissue STABILIZER/765512
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¥4740.00
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2844.00
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3392242852
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4000-520-616
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商品介绍
PAXgene Tissue STABILIZER
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For stabilization of tissue specimens previously fixed in PAXgene Tissue FIX
- Preservation of bothmorphology and biomolecules
- Tissue can be stored and archived for later processing
- RNA, miRNA, DNA, and/orproteins from one sample
PAXgene Tissue STABILIZER is used for transport or storage of tissue specimens fixed with the PAXgene Tissue FIX Container (50 ml). After fixation of tissue samples with the PAXgene Tissue FIX Container (50 ml), PAXgene Tissue FIX is removed and replaced by PAXgene Tissue STABILIZER within the same container. Nucleic acids, proteins and morphology of the sample are now stable up to 7 days at room temperature, or for longer periods at 2–8°C, or even at –20°C and –80°C. Stabilized samples can be embedded in paraffin for histological studies. PAXgene Tissue Kits and supplementary protocols provide efficient subsequent purification of RNA, miRNA, DNA, and/or proteins from the same sample. PAXgene Tissue STABILIZER Concentrate is only to be used in conjunction with tissue specimens previously fixed in PAXgene Tissue FIX.
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Cat No./ID:765512 PAXgene Tissue STABILIZER Concentrate (150 ml) $237.00 8 bottles of PAXgene Tissue Stabilizer concentrate, for 4 liters of PAXgene Tissue STABILIZER |
For Research Use Only. Not for use in diagnostics procedures. No claim or representation is intended to provide information for the diagnosis, prevention, or treatment of a disease.
Product Details
Performance
The fixation and stabilization method preserves tissue morphology and the integrity of nucleic acids without destructive crosslinking and degradation found in formalin-fixed tissues (see figure "Preservation of tissue morphology").When fixed tissue is stored in PAXgene Tissue STABILIZER, the nucleic acids, proteins, and morphology of the tissue sample are stable for up to 7 days at room temperature (15–25°C) or for up to 4 weeks at 2–8°C. Tissue samples can even be stored in the PAXgene Tissue STABILIZER for longer periods at –20°C (–15°C to –30°C) or –80°C (–65°C to –90°C) without negative effects on the morphology of the tissue or the integrity of the nucleic acids.Specifications for storage conditions in PAXgene Tissue STABILIZER were determined using animal tissues.
Principle
The methods for tissue fixation currently used in traditional histology are of limited use for molecular analysis. Fixatives that contain formaldehyde crosslink biomolecules and modify nucleic acids and proteins. Such crosslinks lead to nucleic acid degradation during tissue fixation, storage, and processing. Since they cannot be removed completely, the resulting chemical modifications can cause inhibition in downstream applications, such as quantitative PCR or RT-PCR. To enable both molecular and traditional pathology testing from the same specimen, a method is needed to stabilize molecular content and preserve morphology.
To meet this need, PreAnalytiX has developed the PAXgene Tissue System. The system consists of a fixation reagent (PAXgene Tissue FIX), a stabilization reagent (PAXgene Tissue STABILIZER), prefilled containers for tissue collection, storage, and transportation, and kits for purification of RNA, DNA, or total RNA, including miRNA. In addition, supplementary protocols for protein purification and other applications are available in the "Resources" section of this page or at www.preanalytix.com.
PAXgene Tissue reagents in prefilled containers and PAXgene Tissue Kits provide a complete preanalytical solution for collection, fixation, and stabilization of tissue, and purification of high-quality nucleic acids for molecular research analysis.
Procedure
After fixation of tissue samples with the PAXgene Tissue FIX Container (50 ml), the fixative in the container is removed and replaced by PAXgene Tissue STABILIZER within the same container. Stabilized samples can be embedded in paraffin for histological studies. Nucleic acids and proteins can be isolated from the stabilized samples before or after embedding in paraffin. See the PAXgene Tissue Kit Handbooks for information about DNA, RNA, or miRNA, and the PAXgene Tissue supplementary protocols in the "Resources" section of this page or atwww.preanalytix.com for protein purification and other applications.
Applications
Sections of PAXgene Tissue fixed, paraffin-embedded (PFPE) tissue can be used for histological studies or extraction of nucleic acids or proteins. Purification of RNA, total RNA, including miRNA, or DNA from PAXgene Tissue fixed and stabilized tissue samples requires the use of one of the PAXgene Tissue Kits for RNA, miRNA, or DNA. Purification of protein requires the Qproteome FFPE Kit.
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品牌介绍
QIAGEN是一家专业化致力于生物分子样品制备解决方案的跨国经营企业，总部位于德国。1984年，QIAGEN在德国成立，1996年在美国纽约纳斯达克上市。QIAGEN拥有超过1000项专利和认可证明，在18个国家设立了分公司，代理商服务国超过40个，在全球有超过400000的用户。QIAGEN提供的产品超过500类，包括各种试剂，耗材和自动化纯化工作站。这些产品用于样品采集，稳定，核酸或蛋白的分离，纯化和检测中，不仅广泛的应用于科研领域的各个方面，在生物技术，制药，法医研究，食品安全检测，畜牧业和分子诊断领域也得到了广泛的应用。QIAGEN产品的卓越品质和在应用中的出色表现使得其成为样品处理中标准的代名词。
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