QIAsymphony PAXgene Blood ccfDNA Kit
For purification of ccfDNA from plasma using the QIAsymphony SP
- Optimized chemistry and input volumes for PAXgene Blood ccfDNA Tubes
- Separate protocol lines for small (STA) and large (LAF) ccfDNA fragments
- Prefilled reagent cartrdiges with bar code for safety and ease of use
- Standardized processing for high reproducibility
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- Product Details
- Specifications
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Cat No./ID:768536 QIAsymphony PAXgene Blood ccfDNA Kit (192) $4,060.00 Reagent cartridges, accessories and proteinase K vials for 192 preps |
Product Details
The fully automated QIAsymphony PAXgene Blood ccfDNA Kit procedure generatesccfDNA yields comparable to the manual QIAamp Circulating Nucleic Acid Kit procedure (see figure High performance, comparable to QIAamp Circulating Nucleic Acid Kit). Since yield is strongly dependent of donor, individual yields may vary. Onboard measures of the QIAsymphony SP minimize cross-contamination.
The workflow begins with a centrifugation step that separates plasma from the cellular fraction of the whole blood collected into PAXgene Blood ccfDNA Tubes. The plasma is then carefully transferred into a second tube. Asecond centrifugation step can be carried out optionally to remove any traces of buffy coat. The pure plasma is then transferred to a third tube which is loaded into the QIAsymphony SP.In the QIAsymphony SP, plasma proteins are digested by proteinase K while ccfDNA binds to the surface of magnetic beads (see figure The QIAsymphony SP processes samples containing magnetic particles). Depending on the chosen protocol line, predominantly small ccfDNA fragments (STA protocol line)or small and large ccfDNA fragments (LAF protocol line)are isolated. Three wash steps ensurecontaminant removal. Finally, ccfDNA is eluted from the magnetic particles and is readyfor use in downstream applications (see figure Two protocol lines of the QIAsymphony PAXgene Blood ccfDNA Kit for different research needs).
ccfDNA purified using the QIAsymphony PAXgene Blood ccfDNA Kit and the QIAsymphony SP is ready for usein a wide range of downstream applications, including:
- PCR, digital, multiplex and quantitative real-time PCR
- Pharmacogenomic studies
- SNP genotyping
- Next generation sequencing
- Methylation assays
Specifications
Features | Specifications |
| Applications | Wide spectrum of research applications, including analysis of cancer tumor DNA and non-invasive prenatal testing |
| Elution volume | 60 µl for the STA protocol line 60, 100 or 150 µl for the LAF protocol line |
| Format | Magnetic beads |
| Input volume | 2.4 ml or 4.8 ml plasma |
| Processing | Manual: plasma centrifugation Automated: QIAsymphony SP |
| Shelf life | 6 weeks for an open reagent cartridge |
| Throughput | 192 samples per working day and instrument |
| Time per run | 96 samples of 4.8 ml plasma in 5 h 40 min |
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