Qiagen/PAXgene Blood miRNA Kit/For 50 RNA preps: PAXgene Spin Columns, PAXgene Shredder Spin Columns, Processing Tubes, Microcentrifuge Tubes, RNase-Free DNase, RNase-Free Reagents and Buffers; to be used in conjunction with PAXgene Blood RNA Tubes/763134
市场价：
¥14340.00
美元价：
8604.00
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
PAXgene Blood miRNA Kit
Print
For purification of miRNA and total RNA from whole blood
- Integrated system for collection, stabilization, and purification
- Effective purification of miRNA and total RNA
- RNA stabilization for up to 3 days at 18–25°C
- Stabilization for at least 50 months at –20°C or –70°C
The PAXgene Blood miRNA System consists of the PAXgene Blood miRNA Kit for purification of total RNA, including RNA longer than approximately 18 nucleotides, and PAXgene Blood RNA Tubes (available from BD, cat. no. 762165) for collection, stabilization, and transport of blood. Purification is carried out using silica-based RNA purification technology in a spin-column format. Purification can be carried out manually, using a microcentrifuge, or automated on the QIAcube Connect. Used with the tubes, the kit provides a complete preanalytical solution for collection and stabilization through to purification of high-quality total RNA, including miRNA, for molecular analysis.
- Buy Products
- Product Details
- Specifications
Buy Products
Cat No./ID:763134 PAXgene Blood miRNA Kit (50) $717.00 For 50 RNA preps: PAXgene Spin Columns, PAXgene Shredder Spin Columns, Processing Tubes, Microcentrifuge Tubes, RNase-Free DNase, RNase-Free Reagents and Buffers; to be used in conjunction with PAXgene Blood RNA Tubes |
For Research Use Only. Not for use in diagnostics procedures. No claim or representation is intended to provide information for the diagnosis, prevention, or treatment of a disease.
Product Details
Performance
Blood samples collected in PAXgene Blood RNA Tubes can be safely stored or transported at 15–25°C for up to 72 hours, at 2–8°C for up to 5 days, or at –20°C or –70°C for at least 50 months without showing any significant RNA degradation or changes in transcript levels. Total RNA >18 nucleotides is purified from the stabilized blood samples with the PAXgene Blood miRNA Kit and includes both mRNA and small RNAs such as miRNA (see figure "High yields of small RNA species"). The miRNA species can be analyzed using the QIAGEN miScript PCR System (see figure "Analysis of miRNA species") or other real-time RT-PCR systems.
Principle
Blood samples are collected in PAXgene Blood RNA Tubes. The tubes contain a reagent that lyses blood cells and immediately stabilizes intracellular RNA to preserve the gene expression profile. RNA stabilization is critical for reliable downstream gene expression analysis. Without stabilization, degradation of RNA and upregulation or downregulation of transcripts occur immediately after blood is drawn.
Procedure
With the PAXgene Blood miRNA Kit, total RNA >18 nucleotides (including miRNA) is purified from the stabilized blood samples using well-established PAXgene silica-membrane technology. Purification can be carried out in a manual format or it is automatable on the QIAcube (see figure "The QIAcube").
Applications
The purified total RNA is ready to use and is ideally suited for any downstream application, including:
- RT-PCR and real-time RT-PCR
- Differential display
- cDNA synthesis
- Northern, dot, and slot blot analyses
- Primer extension
- Poly A+ RNA selection
- RNase/S1 nuclease protection
- Microarray analysis
Specifications
Features | Specifications |
| Applications | RT-PCR and real-time RT-PCR, differential display, cDNA synthesis, northern, dot, and slot blot analyses, primer extension, microarray analysis |
| Elution volume | 40 µl |
| Format | Spin column |
| Main sample type | Whole blood |
| Processing | Manual (centrifugation) or on QIAcube |
| Sample amount | 2.5 ml |
| Technology | Silica technology |
| Time per run or per prep | 90 minutes/12 samples |
| Yield | >3 µg (total RNA) |
Product Resources
Product citations in scientific articles
Powered by Bioz
See more details on BiozCustomers who bought these products also bought
- Cat No./ID:762331
PAXgene 96 Blood RNA Kit (4)
4 PAXgene 96 RNA Plates, 4 PAXgene 96 Filter Plates, Buffers, Proteinase K, RNase-free DNase Sets, AirPore Tape Sheets, Collection Vessels. To be used in conjunction with PAXgene Blood RNA Tubes.$3,870.00Add To Cart - Cat No./ID:51185
QIAamp DNA Blood Midi Kit (100)
For 100 DNA midipreps: 100 QIAamp Midi Spin Columns, QIAGEN Protease, Buffers, Collection Tubes (15ml)$977.00Add To Cart - Cat No./ID:951356
MagAttract DNA Blood Midi M48 Kit (192)
MagAttract Suspension B, Buffers, and RNase-Free Water for up to 192 x 350 µl preps$939.00Add To Cart - Cat No./ID:36113
Ni-NTA Magnetic Agarose Beads (6 x 1 ml)
6 x 1 ml nickel-charged magnetic agarose beads (5%suspension)$703.00Add To Cart - Cat No./ID:761133
PAXgene Blood DNA Kit (25)
Processing tubes and buffers for 25 preparations$366.00Add To Cart - Cat No./ID:990399
Spin Column Adapter Rings (25)
Spin column adapter rings for use with spin columns that have screw caps; not required for all protocols$149.00Add To Cart - Cat No./ID:762164
PAXgene Blood RNA Kit (50)
50 PAXgene Spin Columns, 50 PAXgene Shredder Spin Columns, Processing Tubes, RNase-Free DNase I, RNase-Free Reagents and Buffers. To be used in conjunction with PAXgene Blood RNA Tubes$588.00Add To Cart - Cat No./ID:217184
miRNeasy Serum/Plasma Kit (50)
For 50 total RNA preps: 50 RNeasy MinElute Spin Columns, Collection Tubes (1.5 ml and 2 ml), QIAzol Lysis Reagent, Ce_miR-39_1 miScript Primer Assay, RNase-free Reagents and Buffers$489.00Add To Cart - Cat No./ID:51194
QIAamp DNA Blood Maxi Kit (50)
For 50 DNA maxipreps: 50 QIAamp Maxi Spin Columns, QIAGEN Protease, Buffers, Collection Tubes (50 ml)$990.00Add To Cart - Cat No./ID:9014579
QIAvac Multiwell
Vacuum manifold for processing 96-well purification plates of SBS standard$888.00Add To Cart - Cat No./ID:80224
AllPrep DNA/RNA/miRNA Universal Kit (50)
For 50 preps: 50 AllPrep DNA Mini Spin Columns, 50 RNeasy Mini Spin Columns, Collection Tubes, and Buffers$676.00Add To Cart - Cat No./ID:764133
PAXgene Bone Marrow RNA Kit (30)
For 30 RNA preps: 30 PAXgene Spin Columns, 30PAXgene Shredder Spin Columns, Processing Tubes, RNase-free DNase I, RNase-free reagents and buffers. To be used in conjunction with PAXgene Bone Marrow RNA Tubes$707.00Add To Cart - Cat No./ID:34924
Polypropylene Columns (1 ml)
50/pack, 1 ml capacity$147.00Add To Cart - Cat No./ID:32915
N-Terminus pQE Vector Set
25 µg each: pQE-9, pQE-30, pQE-31, pQE-32, pQE-40$718.00Add To Cart - Cat No./ID:59826
EpiTect Fast DNA Bisulfite Kit (200)
For 200 preps: Bisulfite Solution, DNA Protect Buffer, MinElute DNA Spin Columns, Carrier RNA, and Buffers$785.00Add To Cart - Cat No./ID:1022064
GFP-22 siRNA (5 nmol)
Control siRNA targeting green fluorescent protein$182.00Add To Cart - Cat No./ID:76603
miRCURY Exosome Serum/Plasma Kit
For isolation of exosomes and other extracellular vesicles from serum or plasma; includes 10 ml Precipitation Buffer A, 10 ml Resuspension Buffer, 200 U Thrombin and 500 µl Thrombin Buffer$335.00Add To Cart - Cat No./ID:217504
miRNeasy FFPE Kit (50)
50 RNeasy MinElute Spin Columns, Collection Tubes, Proteinase K, RNase-Free DNase I, DNase Booster Buffer, RNase-Free Buffers, RNase-Free Water$460.00Add To Cart
品牌介绍
QIAGEN是一家专业化致力于生物分子样品制备解决方案的跨国经营企业，总部位于德国。1984年，QIAGEN在德国成立，1996年在美国纽约纳斯达克上市。QIAGEN拥有超过1000项专利和认可证明，在18个国家设立了分公司，代理商服务国超过40个，在全球有超过400000的用户。QIAGEN提供的产品超过500类，包括各种试剂，耗材和自动化纯化工作站。这些产品用于样品采集，稳定，核酸或蛋白的分离，纯化和检测中，不仅广泛的应用于科研领域的各个方面，在生物技术，制药，法医研究，食品安全检测，畜牧业和分子诊断领域也得到了广泛的应用。QIAGEN产品的卓越品质和在应用中的出色表现使得其成为样品处理中标准的代名词。
联络我们
