RNeasy Protect Bacteria Mini Kit
- Immediate in vivo RNA stabilization and protection
- Reliable gene expression and gene-profiling data
- No need for liquid nitrogen, dry ice, or phenol
- Ready-to-use, high-quality total RNA in minutes
- No CsCl gradients, no LiCl or ethanol precipitation
The RNeasy Protect Bacteria Mini Kit includes RNAprotect Bacteria Reagent for stabilizing RNA in bacterial samples, and RNeasy spin columns for purifying up to 100 µg of high-quality RNA using silica-membrane technology. Efficient disruption of bacterial samples can be achieved using the TissueLyserII. The kit can be automated on theQIAcube Connect.
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Cat No./ID:74524 RNeasy Protect Bacteria Mini Kit (50) $528.00 RNeasy Mini Kit (50) and RNAprotect Bacteria Reagent (2 x 100 ml) |
Product Details
Two volumes of RNAprotect Bacteria Reagent are added directly to 1 volume of bacterial culture (≤7.5 x 108 bacteria) prior to RNA isolation, providing immediate stabilization of RNA (see flowchart "RNAprotect Bacteria Reagent procedure"). The stabilization allows time for efficient bacterial lysis using a choice of protocols: enzymatic lysis, mechanical disruption, or a combination of both methods. We recommend the TissueLyser II for efficient mechanical disruption. Ethanol is then added to the lysate to provide ideal binding conditions. The lysate is loaded onto the RNeasy silica membrane (binding capacity 100 µg RNA). Following RNA binding, all contaminants are efficiently washed away. Pure, concentrated RNA is eluted in 30–100 µl of water. The RNeasy Protect Bacteria Mini Kit can be automated on the QIAcube.
Amounts of RNA isolated from bacteria can vary due to species and growth conditions. The RNeasy Protect Bacteria Mini Kit is suitable for use with a wide range of bacterial species, both Gram positive (e.g., Staphylococcus aureus and Mycobacterium avium) and Gram negative (e.g., Escherichia coli and Salmonella typhimurium). Bacteria grown in either minimal or complex medium can be used. Since the RNeasy procedure enriches for RNA species >200 nucleotides, RNA yield does not include 5S rRNA, tRNAs, or other low-molecular-weight RNAs. RNeasy Protect Bacteria Kits provide the highest-quality RNA with minimum copurification of DNA. For certain RNA applications that are sensitive to very small amounts of DNA, the residual amounts of DNA remaining can be removed using the RNase-Free DNase Set for convenient on-column DNase treatment during the RNeasy procedure.
RNA purified with the RNeasy Protect Bacteria Mini Kit is ideal for use in all applications. Downstream applications include:
- Northern, dot, and slot blotting
- End-point RT-PCR
- Quantitative, real-time RT-PCR
- Array analysis
Specifications
Features | Specifications |
| Applications | PCR, qPCR, real-time RT-PCR, microarray |
| Elution volume | 30–100 µl |
| Format | Spin column |
| Main sample type | Bacteria |
| Processing | Manual (centrifugation) |
| Purification of total RNA, miRNA, poly A+ mRNA, DNA or protein | RNA |
| Sample amount | 15 –100 µg |
| Stabilization | Yes |
| Technology | Silica technology |
| Time per run or per prep | 20 minutes |
| Yield | 8–70 µg |
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