RNeasy Protect Cell Mini Kit
- Immediate RNA stabilization in sorted or cultured cells
- No need to remove medium prior to cell stabilization
- Storage and shipping of cells without dry ice
- Archiving of cells at –20ºC or –80ºC
- Purification of high-quality RNA in minutes
The RNeasy Protect Cell Mini Kit includes RNAprotect Cell Reagent, which stabilizes RNA and DNA in sorted or cultured cells and allows convenient and reliable transport of the cells at ambient temperature. The stabilized cells can be stored or shipped at 30°C (1 day), 15–25°C (7 days), or 2–8°C (4 weeks). The RNeasy Protect Cell Mini Kit provides the RNeasy Plus Mini Kit for purification of total RNA. Stabilized cells are lysed and spun through gDNA Eliminator spin columns to effectively remove genomic DNA. High-quality RNA is then purified using RNeasy spin columns. The purification procedure can be automated on the QIAcube.
- Buy Products
- Product Details
- Specifications
Buy Products
Cat No./ID:74624 RNeasy Protect Cell Mini Kit (50) $391.00 RNAprotect Cell Reagent (50 ml) and RNeasy Plus Mini Kit (50) |
Product Details
The RNeasy Protect Cell Mini Kit is ideal for gene expression analysis experiments where cells need to be delivered from one lab to another (see Table 1).
| Assay result | RNAprotect Cell Reagent | RNAprotect TissueReagent |
|---|---|---|
| RNA yield using RNeasy technology (µg) | 10 | 3.5 |
| Real-time RT-PCR of abl (CT value) | 25 | 26 |
Cells in RNAprotect Cell Reagent can be stored or transported for up to 1 day at 30ºC, up to 7 days at room temperature (15–25ºC), or up to 4 weeks at 2–8ºC, and can also be stored long-term at –20ºC or –80ºC (see Table 2, and figures "Effective inhibition of PMA induction" and "More reliable quantification of CD83 transcript").
| Cell storage conditions | RIN value of purified RNA* |
|---|---|
| 30ºC (1 day) | 9.8 |
| 25ºC (7 days) | 9.7 |
| 2–8ºC (28 days) | 10 |
| –20ºC (28 days) | 9.8 |
| –80ºC (28 days) | 9.8 |
The RNeasy Protect Cell Mini Kit provides a complete RNA protection and isolation solution, from sample harvesting to pure RNA, in one kit. Proven RNeasy technology, combined with the RNA-stabilizing properties of RNAprotect Cell Reagent, allows purification of high-quality, intact RNA. Sorted or cultured cells are mixed with RNAprotect Cell Reagent, which immediately stabilizes the cellular RNA, ensuring reliable results in downstream gene expression analysis. RNAprotect Cell Reagent can be added directly to cells in culture medium or buffer, eliminating the need to wash or process cells (e.g., by trypsin treatment). Alternatively, RNAprotect Cell Reagent can be added to pelleted cells. Following stabilization, RNeasy technology simplifies total RNA isolation by combining the stringency of guanidine-isothiocyanate lysis with the speed and purity of silica-membrane purification.
Cells in RNAprotect Cell Reagent can be transported at ambient temperature, avoiding the need to freeze cell pellets in liquid nitrogen and to ship the frozen pellets on dry ice. RNA purified using the RNeasy Plus Mini Kit is ideally suited for downstream applications that are sensitive to low amounts of DNA contamination, such as:
- Quantitative real-time RT-PCR
Specifications
Features | Specifications |
| Applications | PCR, qPCR, real-time RT-PCR, microarray |
| Elution volume | 30–50 µl |
| Format | Spin column |
| Main sample type | Cultured cells |
| Processing | Manual (centrifugation) |
| Purification of total RNA, miRNA, poly A+ mRNA, DNA or protein | Total RNA |
| Sample amount | Max. 10e7 cells |
| Stabilization | Yes |
| Technology | Silica technology |
| Yield | Varies |
Product Resources
Customers who bought these products also bought
- Cat No./ID:76526
RNAprotect Cell Reagent (250 ml)
250 ml RNAprotect Cell Reagent$363.00Add To Cart - Cat No./ID:9018906
36-Well Rotor Locking Ring
For locking PCR Tubes, 0.2 ml, in the 36-Well Rotor$209.00Add To Cart - Cat No./ID:9018907
36-Well Rotor
For holding PCR Tubes, 0.2 ml, with reaction volumes of 20–50 µl; requires 36-Well Rotor Locking Ring$553.00Add To Cart - Cat No./ID:382615
Investigator 26plex QS Kit (100)
Primer Mix, Fast Reaction Mix 3.0includingTaqDNA Polymerase, Control DNA, allelic ladder 26plex, and nuclease-free water
$2,344.00Add To Cart - Cat No./ID:972807
PyroMark Gold Q96 Reagents (50 x 96)
For performing Pyrosequencing reactions on the PyroMark Q96 MD$1,653.00Add To Cart - Cat No./ID:51161
QIAamp 96 DNA Blood Kit (4)
For 4 x 96 DNA preps: 4 QIAamp 96 Plates, QIAGEN Protease, Reagents, Buffers, Lysis Blocks, Tape Pads, Collection Vessels$1,305.00Add To Cart - Cat No./ID:28183
QIAquick 96 PCR Purification Kit (24)
For purification of 24 x 96 PCR reactions: 24 QIAquick 96 Plates, Buffers, Collection Microtubes (1.2 ml), Caps$3,326.00Add To Cart - Cat No./ID:77164
exoRNeasy Maxi Kit (50)
For purification of total exosome-derived RNA; includes 50 exoEasy Maxi columns and 50 RNeasy MinElute spin columns$2,305.00Add To Cart - Cat No./ID:158912
Protein Precipitation Solution (350 ml)
350 ml Protein Precipitation Solution$417.00Add To Cart - Cat No./ID:14700-50-NF
RNeasy PowerWater Kit (50)
For the isolation of total RNA from filtered water samples, including turbid water$560.00Add To Cart - Cat No./ID:13000-V1-5
Vortex Adapter for 6 (5 ml) tubes
For vortexing 5 and 15 ml tubes using the Vortex-Genie 2 Vortex.$126.00Add To Cart - Cat No./ID:386015
DNA Size Standard 550 (BTO) (100)
DNA Size Standard 550 (BTO) for 100 reactions$134.00Add To Cart - Cat No./ID:55284
QIAamp MinElute ccfDNA Midi Kit (50)
For 50 preps (4 or 5 ml sample input volume each): QIAamp UCP MinElute Columns, QIAGEN Proteinase K, Magnetic Bead Suspension, Buffers, Bead Elution Tubes, and Collection Tubes (1.5 ml and 2 ml)$977.00Add To Cart - Cat No./ID:12243
QIAfilter Plasmid Midi Kit (25)
25 QIAGEN-tip 100, Reagents, Buffers, 25 QIAfilter Midi Cartridges$360.00Add To Cart - Cat No./ID:239045
GelPilot 100 bp Plus Ladder (100)
Ready-to-use DNA marker; 600 µl (100 lanes), 11 fragments: 100–1500 bp$176.00Add To Cart - Cat No./ID:74524
RNeasy Protect Bacteria Mini Kit (50)
RNeasy Mini Kit (50) and RNAprotect Bacteria Reagent (2 x 100 ml)$528.00Add To Cart - Cat No./ID:158924
RNase A Solution (5 ml)
5 ml RNase A Solution$446.00Add To Cart
