QIAprep Spin Miniprep Columns
For purification of up to 20 μg molecular biology grade plasmid DNA
- Ready-to-use plasmid DNA in minutes
- Reproducible yields of molecular biology grade plasmid DNA
- Single protocol for high- and low-copy vectors
The QIAprep Spin Miniprep Kit is designed for isolation of up to 20 μg high-purity plasmid or cosmid DNA for use in routine molecular biology applications such as fluorescent and radioactive sequencing and cloning.
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Cat No./ID:27115 QIAprep 2.0Spin Miniprep Columns (100 spin columns) $98.90 For high-purity plasmid minipreps |
Product Details
The QIAprep Spin Miniprep Kit enables purification of up to 20 μg molecular biology grade plasmid DNA or cosmid DNA for use in routine molecular biology applications such as PCR, sequencing and cloning. Versatile QIAprep spin columns can be used either in microcentrifuges, on vacuum manifolds, or in the QIAcube (see figures "QIAprep Spin Column handling options A, B, and C"). The vacuum procedure provides simplified handling and faster sample processing. QIAprep spin columns can be vacuum processed using the QIAvac 24 Plus or any other commercial manifold with luer connectors. The QIAprep Spin Miniprep Kit can now also be fully automated on the QIAcube.
| Format: | Spin columns |
| Purification module: | QIAprep spin columns |
| Throughput: | 1–24 samples |
| Preparation time: | 24 minipreps in 30 minutes |
| Equipment required: | Microcentrifuge or vacuum manifold; fully automatable using the QIAcube |
| Lysate clearing: | Centrifugation |
| Capacity of column reservoir: | 800 µl |
| Minimum elution buffer volume: | 50 µl |
| Culture volume for high-copy plasmids: | 1–5 ml |
| Culture volume for low-copy plasmids/cosmids: | 1–10 ml |
Purified DNA can be used in restriction digestion (see figure "Complete digestion with various restriction enzymes").
Plasmid purification using QIAprep Kits follows a simple bind-wash-elute procedure (see flowchart "The QIAprep procedure"). First, bacterial cultures are lysed and the lysates are cleared by centrifugation. The cleared lysates are then applied to the QIAprep module where plasmid DNA adsorbs to the silica membrane. Impurities are washed away and pure DNA is eluted in a small volume of elution buffer or water.
In addition to plasmid purification from Escherichia coli, QIAprep Kits can be used to purify plasmid DNA from Saccharomyces cerevisiae, Bacillus subtilis, and Agrobacterium tumefaciens. Contact QIAGEN Technical Services or your local distributor for protocols for these applications.
The QIAprep Miniprep Kits provides reproducible yields of high-purity DNA suitable for use in most applications, including:
- PCR
- Restriction digestion
- Ligation and transformation
- Sequencing
- Screening
Specifications
Features | Specifications |
| Applications | Fluorescent and radioactive sequencing (including capillary sequencing), ligation, cloning, transformation etc. |
| Culture volume/starting material | 1–10 ml culture volume |
| Elution volume | 50 µl (minimal) |
| Plasmid type | High-copy, low-copy, cosmid DNA |
| Processing | Manual (vacuum or centrifugation) |
| Samples per run (throughput) | 1–24 samples per run |
| Technology | Silica technology |
| Time per run or prep per run | <30> |
| Yield | <20> |
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