Qiagen/Puregene Accessories/450 ml RBC Lysis Solution/158902
市场价：
¥1940.00
美元价：
1164.00
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
Puregene Accessories
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For purification of archive-quality DNA from a wide range of sample types
- Archive-quality DNA for long-term storage
- Reproducible DNA purification
- Convenient, scalable purification procedure
Puregene Accessories used in combination with Gentra Puregene Kits enable purification of high-molecular-weight (100–200 kb) DNA suitable for archiving. The scalable purification procedure gently removes contaminants and inhibitors and allows large-volume samples to be purified for use as long-term references.
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- Product Details
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Cat No./ID:158902 RBC Lysis Solution (450 ml) $97.00 450 ml RBC Lysis Solution |
Cat No./ID:158904 RBC Lysis Solution (1000 ml) $151.00 1000 ml RBC Lysis Solution |
Cat No./ID:158906 Cell Lysis Solution (125 ml) $91.10 125 ml Cell Lysis Solution |
Cat No./ID:158908 Cell Lysis Solution (1000 ml) $474.00 1000 ml Cell Lysis Solution |
Cat No./ID:158910 Protein Precipitation Solution (50 ml) $91.50 50 ml Protein Precipitation Solution |
Cat No./ID:158912 Protein Precipitation Solution (350 ml) $417.00 350 ml Protein Precipitation Solution |
Cat No./ID:158914 DNA Hydration Solution (100 ml) $83.50 100 ml DNA Hydration Solution |
Cat No./ID:158916 DNA Hydration Solution (500 ml) $285.00 500 ml DNA Hydration Solution |
Cat No./ID:158918 Puregene Proteinase K (650 µl) $87.50 650 µl Puregene Proteinase K |
Cat No./ID:158920 Puregene Proteinase K (5 ml) $446.00 5 ml Puregene Proteinase K |
Cat No./ID:158922 RNase A Solution (650 µl) $87.50 650 µl RNase A Solution |
Cat No./ID:158924 RNase A Solution (5 ml) $446.00 5 ml RNase A Solution |
Cat No./ID:158926 Cell Suspension Sol. (125 ml) $88.80 Cell Suspension Sol. (125 ml) |
Cat No./ID:158928 Lytic Enzyme Solution (650 µl) $84.80 650 µl Lytic Enzyme Solution |
Cat No./ID:158930 Glycogen Solution (500 µl) $76.20 500 µl Glycogen Solution |
Cat No./ID:158932 Clotspin Baskets (50) $158.00 50 Clotspin Baskets |
Product Details
Principle
Using Gentra Puregene Kits, cells are lysed with an anionic detergent in the presence of a DNA stabilizer. The DNA stabilizer limits the activity of intracellular DNases and also DNases found elsewhere in the environment. RNA is then removed by treatment with an RNA digesting enzyme. Other contaminants, such as proteins, are removed by salt precipitation. Finally, the genomic DNA is recovered by precipitation with alcohol and dissolved in hydration solution (1 mM EDTA, 10 mM Tris·Cl pH 7.5). Purified DNA typically has an A260/A280 ratio between 1.7 and 1.9, and is up to 200 kb in size. The DNA can be safely stored at 2–8°C, –20°C, or –80°C.
Procedure
Archive-quality DNA
Purity of DNA has a significant effect on the accuracy of results obtained in downstream applications. Sensitive downstream applications, such as PCR, demand the use of DNA of the highest quality and molecular weight for reliable results. Gentra Puregene Kits removes contaminants and enzyme inhibitors enabling purification of highly stable DNA well-suited for archiving. Purified DNA is of high quality demonstrated by molecular weights of 100–200 kb.
Applications
DNA purified using Puregene Accessories and Gentra Puregene Kits is highly stable and suited for use in a wide range of applications, such as:
- DNA archiving
- PCR
- SNP analysis
- Southern blotting
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品牌介绍
QIAGEN是一家专业化致力于生物分子样品制备解决方案的跨国经营企业，总部位于德国。1984年，QIAGEN在德国成立，1996年在美国纽约纳斯达克上市。QIAGEN拥有超过1000项专利和认可证明，在18个国家设立了分公司，代理商服务国超过40个，在全球有超过400000的用户。QIAGEN提供的产品超过500类，包括各种试剂，耗材和自动化纯化工作站。这些产品用于样品采集，稳定，核酸或蛋白的分离，纯化和检测中，不仅广泛的应用于科研领域的各个方面，在生物技术，制药，法医研究，食品安全检测，畜牧业和分子诊断领域也得到了广泛的应用。QIAGEN产品的卓越品质和在应用中的出色表现使得其成为样品处理中标准的代名词。
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