QIAzol Lysis Reagent
- Optimized lysis conditions to purify RNA for gene expression analysis
- High yields of RNA from fatty tissues
- Easy-to-follow protocol for lysis and homogenization
- Integration with RNeasy cleanup to prevent phenol carryover
- Compatibility with a variety of tissue types
QIAzol Lysis Reagent is optimized for lysis of fatty tissues. The standard QIAzol protocol provides an easy-to-follow procedure, combining QIAzol lysis with standard homogenization methods and isopropanol precipitation.
- Buy Products
- Product Details
Buy Products
Cat No./ID:79306 QIAzol Lysis Reagent (200ml) $336.00 200 ml QIAzol Lysis Reagent |
Product Details
Phenol/guanidine-based QIAzol Lysis Reagent can be used to lyse all classes of tissues but is optimized for lysis of fatty tissues, such as brain and adipose tissues. The combination of organic extraction and chaotropic disruption contributes to efficient lysis and higher yields of total RNA. The organic extraction step removes both proteins and DNA, improving the efficiency of further purification steps, for example, precipitation or spin columns. QIAGEN provides several solutions for efficient RNA purification, which integrate QIAzol lysis and RNeasy silica membrane purification technologies in single, streamlined protocols (e.g., RNeasy Plus Universal Kits for any type of tissue).
The standard QIAzol protocol provides an easy-to-follow procedure, combining QIAzol lysis with standard homogenization methods and isopropanol precipitation. Tissue samples are homogenized in QIAzol Lysis Reagent. After addition of chloroform, the homogenate is separated into aqueous and organic phases by centrifugation. RNA partitions to the upper, aqueous phase while DNA partitions to the interphase and proteins to the lower, organic phase. RNA is precipitated from the aqueous phase by addition of isopropanol. The pellet is then washed with ethanol and redissolved in RNase-free water. For high performance in downstream applications, subsequent purification of the RNA is recommended using the RNeasy MinElute Cleanup Kit to remove any contaminating phenol and to concentrate the RNA.
Phenol/guanidine-based QIAzol Lysis Reagent is suitable for all tissues and is optimized for lysis of fatty tissues, such as brain and adipose tissues. We recommend purification of the RNA product with an RNeasy Plus Universal Kit, or the RNeasy MinElute Cleanup Kit, for high performance in any downstream application.
Product Resources
Product citations in scientific articles
Customers who bought these products also bought
- Cat No./ID:74106
RNeasy Mini Kit (250)
250 RNeasy Mini Spin Columns, Collection Tubes (1.5 ml and 2 ml), RNase-free Reagents and Buffers$1,451.00Add To Cart - Cat No./ID:79254
RNase-Free DNase Set (50)
1500 Kunitz units RNase-free DNase I, RNase-free Buffer RDD, and RNase-free water for 50 RNA minipreps$113.00Add To Cart - Cat No./ID:217184
miRNeasy Serum/Plasma Kit (50)
For 50 total RNA preps: 50 RNeasy MinElute Spin Columns, Collection Tubes (1.5 ml and 2 ml), QIAzol Lysis Reagent, Ce_miR-39_1 miScript Primer Assay, RNase-free Reagents and Buffers$489.00Add To Cart - Cat No./ID:1067933
Buffer RWT (80 ml)
80 ml Buffer RWT concentrate$135.00Add To Cart - Cat No./ID:217004
miRNeasy Mini Kit (50)
For 50 preps: 50 RNeasy Mini Spin Columns, Collection Tubes (1.5 ml and 2 ml), QIAzol Lysis Reagent, RNase-Free Reagents and Buffers$390.00Add To Cart - Cat No./ID:74804
RNeasy Lipid Tissue Mini Kit (50)
50 RNeasy Mini Spin Columns, Collection Tubes (1.5 ml and 2 ml), QIAzol Lysis Reagent, RNase-free Reagents and Buffers$425.00Add To Cart - Cat No./ID:69989
Stainless Steel Beads, 5 mm (200)
200 stainless steel beads (5 mm diameter), suitable for use with TissueLyser systems$208.00Add To Cart - Cat No./ID:74104
RNeasy Mini Kit (50)
50 RNeasy Mini Spin Columns, Collection Tubes (1.5 ml and 2 ml), RNase-free Reagents and Buffers$330.00Add To Cart - Cat No./ID:76106
RNAprotect Tissue Reagent (250ml)
250 ml RNAprotect TissueReagent for stabilization of RNA in 125 x 200 mg tissue samples
$369.00Add To Cart - Cat No./ID:217084
miRNeasy Micro Kit (50)
For 50 total RNA preps: 50 RNeasy MinElute Spin Columns, Collection Tubes (1.5 ml and 2 ml), QIAzol Lysis Reagent, RNase-Free Reagents and Buffers$411.00Add To Cart - Cat No./ID:74004
RNeasy Micro Kit (50)
50 RNeasy MinElute Spin Columns, Collection Tubes (1.5ml and 2ml), RNase-free DNase I, Carrier RNA, RNase-free Reagents and Buffers$522.00Add To Cart - Cat No./ID:74171
RNeasy 96 QIAcube HT Kit (5)
For 480 preps: RNeasy 96 plates, RNase-free water, buffers$1,384.00Add To Cart - Cat No./ID:69104
DNeasy Plant Mini Kit (50)
50 DNeasy Mini Spin Columns, 50 QIAshredder Mini Spin Columns, RNase A, Buffers, Collection Tubes (2 ml)$243.00Add To Cart - Cat No./ID:70042
Oligotex mRNA Midi Kit (12)
For 12 mRNA midipreps: 700 µl Oligotex Suspension, Small Spin Columns, Collection Tubes (1.5 ml), RNase-Free Reagents and Buffers$862.00Add To Cart - Cat No./ID:74134
RNeasy Plus Mini Kit (50)
For 50 minipreps: RNeasy Mini Spin Columns, gDNA Eliminator Spin Columns, Collection Tubes, RNase-Free Water and Buffers$374.00Add To Cart - Cat No./ID:51306
QIAamp DNA Mini Kit (250)
For 250 DNA preps: 250 QIAamp Mini Spin Columns, QIAGEN Proteinase K, Reagents, Buffers, Collection Tubes (2 ml)$757.00Add To Cart - Cat No./ID:19566
Collection Microtube Caps (120 x 8)
Nonsterile polypropylene caps for collection microtubes (1.2 ml) and round-well blocks, 960 in strips of 8$32.80Add To Cart - Cat No./ID:990381
Sample Tubes RB (2 ml)
1000 safe-lock microcentrifuge tubes (2 ml) for use with the QIAcube and QIAcube Connect$120.00Add To Cart
