Qiagen/exoEasy Maxi Kit/For 20 vesicle preps: 20 exoEasy Maxi Spin Columns, Collection Tubes (50 ml), Reagents and Buffers/76064
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¥14780.00
美元价：
8868.00
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3392242852
电话号码：
4000-520-616
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info@ebiomall.com
商品介绍
exoEasy Maxi Kit
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For purification of exosomes and other extracellular vesicles (EVs) from plasma, serum and cell culture supernatant
- Isolate intact exosomes and other extracellular vesicles
- Suitable for functional testing and physical and biochemical analysis
- Purify exosomes and other extracellular vesicles in 25 minutes
- Process multiple samples in parallel using a simple spin-column procedure
- Use with up to 4 ml of plasma or serum or 32 ml of cell culture supernatant
The exoEasy Maxi Kit uses membrane affinity spin columns to efficiently isolate exosomes and other extracellular vesicles from serum, plasma, cell culture supernatant and other biological fluids. The maxi column format allows the use of large sample volumes, including up to 4 ml plasma or serum or up to 32 ml cell culture supernatant. The procedure is very fast, consistent and highly suited for isolation of EVs for functional analysis of exosomes and other extracellular vesicles.
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- Product Details
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Cat No./ID:76064 exoEasy Maxi Kit (20) $739.00 For 20 vesicle preps: 20 exoEasy Maxi Spin Columns, Collection Tubes (50 ml), Reagents and Buffers |
Cat No./ID:76204 Buffer XBP (250 ml) $84.90 250 ml Binding Buffer |
The exoEasy Maxi Kit is intended for molecular biology applications. This product is not intended for the diagnosis, prevention, or treatment of a disease.
Product Details
Performance
The exoEasy protocol for purification of exosomes and other extracellular vesicles is not only faster than ultracentrifugation, but also yields a cleaner preparation. Scanning electron microscopy shows that while both techniques deliver intact vesicles of the expected size, the preparation from ultracentrifugation also contains many smaller structures that do not match the expected size or shape for EVs (see figure “Intact vesicles are eluted from the exoEasy membrane with higher purity compared with ultracentrifugation”).Isolated vesicles are fully functional and can be used for several downstream applications including cell culture work (see figure “Extracellular vesicles isolated using the exoEasy Kit are taken up efficiently by target cells (customer data)”). Quality and quantity of eluted vesicles can be determined by a range of different methods. These include electron microscopy, physical characterization (e.g., Nanoparticle Tracking Analysis (NTA), or Tunable Resistive Pulse Sensing (TRPS)) and analysis of molecular constituents of vesicles, such as proteins, nucleic acids or lipids. Since particle counting techniques like NTA or TRPS do not distinguish vesicles from other particulate matter, particle counts performed in unprocessed biological fluids or crude preparations that still contain other particulate matter (e.g., protein complexes) may vastly overestimate the number of vesicles present.
Principle
The exoEasy Kit improves upon traditional ultracentrifugation exosome or microvesicle isolation methods, yielding purified extracellular vesicles in just 25 minutes. The exoEasy Maxi Kit uses a membrane-based affinity binding step to isolate exosomes and other EVs from serum and plasma or cell culture supernatant. The method does not distinguish EVs by size or cellular origin, and is not dependent on the presence of a particular epitope. Instead, it makes use of a generic, biochemical feature of vesicles to recover the entire spectrum of extracellular vesicles present in a sample (see figure “Characterization of extracellular vesicles isolated using the exoEasy Maxi Kit byNTA”). It is therefore essential to completely remove cells, cell fragments, apoptotic bodies, etc., by centrifugation or filtration of samples before starting the protocol.The technology developed with Exosome Diagnostics, Inc., uses a spin column format and specialized buffers to purify exosomes from pre-filtered biological fluids; up to 4 ml using plasma or serum. For cell culture supernatants, processing of up to 32 ml sample (4 column loading steps) has been successfully tested. However, the concentration of vesicles in supernatants depends strongly on the cell type and culture conditions; therefore, we recommend starting with no more than 16 ml of supernatant for sample material that has not been tested with the kit previously. Higher sample volumes may result in reduced recovery of vesicles. The procedure is very fast, consistent and highly suited for functional analysis of exosomes and other extracellular vesicles. Particulate matter other than vesicles, such as larger protein complexes that are especially abundant in plasma and serum, is largely removed during the binding and wash steps.
Procedure
The total procedure for isolating extracellular vesicles takes just 25 minutes. Pre-filtered plasma, serum or cell culture supernatant (with particles larger than 0.8 μm excluded) are mixed with Buffer XBP and bound to an exoEasy membrane affinity spin column. The bound exosomes are washed with Buffer XWP, eluted with 400 µl Buffer XE (an aqueous buffer containing primarily inorganic salts) and are then ready to use in physical or biochemical analysis. For certain applications, such as biological assays, an additional concentrating or buffer exchange step may be required. This can be achieved using ultrafiltration with a pore size of 100 kDa or smaller.
Applications
The exoEasy Kit is highly suited for isolating microvesicles found in serum, plasma, cell culture supernatant and other biological fluids. Isolated vesicles can be analyzed by and used for:
- Physical characterization (e.g., NTA, TRPS)
- Electron microscopy
- Isolation and analysis of DNA, RNA*, proteins, lipids and other constituents
- Flow cytometry
- Antibody- or fluorescent dye-based labeling
- Uptake by recipient cells
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品牌介绍
QIAGEN是一家专业化致力于生物分子样品制备解决方案的跨国经营企业，总部位于德国。1984年，QIAGEN在德国成立，1996年在美国纽约纳斯达克上市。QIAGEN拥有超过1000项专利和认可证明，在18个国家设立了分公司，代理商服务国超过40个，在全球有超过400000的用户。QIAGEN提供的产品超过500类，包括各种试剂，耗材和自动化纯化工作站。这些产品用于样品采集，稳定，核酸或蛋白的分离，纯化和检测中，不仅广泛的应用于科研领域的各个方面，在生物技术，制药，法医研究，食品安全检测，畜牧业和分子诊断领域也得到了广泛的应用。QIAGEN产品的卓越品质和在应用中的出色表现使得其成为样品处理中标准的代名词。
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