Qiagen/miRCURY LNA miRNA Focus PCR面板/339325
市场价：
¥0.00
美元价：
0.00
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
miRCURY LNA miRNA Focus PCR Panels
Print
For exceptionally sensitive and specific miRNA profiling using LNA-enhanced, SYBR® Green-based miRNA qPCR
- Ready-to-use miRNA PCR panels focused on particular applications, sample types or diseases
- Fast and easy to use with no pre-amplification required
- Simple protocol for high reproducibility and minimal technical variation
- Reliable discrimination of closely related miRNAs and mature and precursor miRNAs
miRCURY LNA miRNA Focus PCR Panels enable exceptionally sensitive and specific miRNA profiling with the miRCURY LNA miRNA PCR System. These PCR primer panels are enhanced withLNA technology andare specific for particular sample types, diseases or pathways, providing a flexible solution that allows you to focus on the miRNAs relevant to your application. miRCURY LNA miRNA Focus PCR Panels are pre-aliquoted in 96- or 384-well PCR plates and are ready for use.
Need a quote for your research project or would you like to discuss your project with our specialist team? Just contact us!
- Buy Products
- Product Details
Buy Products
Cat No./ID:339325 miRCURY LNA miRNA Focus PCR Panels Go to GeneGlobemiRCURY LNA miRNA PCR Panels for application-based miRNome profiling, available in 96-well or 384-well format; for SYBR® Green-based detection |
miRCURY LNA miRNA Focus PCR Panels are intended for molecular biology applications. These products are not intended for the diagnosis, prevention, or treatment of a disease.
Product Details
Performance
Serum/Plasma miRNA PCR Panels
Why study miRNAs in serum and plasma?miRNAs are present in small amounts in serum and plasma. They circulate in the blood in a highly stable, cell-free form, suggesting that they are protected from the RNA-degrading enzymes found in blood (see figuresmiRNAs are stable in serum and plasma and Complete miRNA serum profile).miRNA profiles have been shown to be altered in serum and plasma taken from patients suffering from cancer and other diseases. This makes miRNAs promising candidates for use in non-invasive discovery of important new biomarkers for a wide range of diseases and biological processes. Blood samples are easy to obtain and carry little risk to the patient, compared with biopsies and surgical procedures.Challenges of miRNA profiling in serum and plasmaSerum and plasma only contain a fraction of the RNA found in whole blood (see figure Blood serum and plasma contain small amounts of RNA). Most of the RNA is found in the buffy coat, which mainly consists of white blood cells and platelets. To get accurate miRNA profiles, it is important to ensure that the serum/plasma is not contaminated by RNA from blood cells, as this would skew the results of the experiment.Several other challenges exist when profiling miRNAs in serum and plasma, including:- Small amounts of RNA prevent use of standard RNA quality control methods
- RNA from serum/plasma contains inhibitors of PCR enzymes
- Standard reference genes, such as U6, are not present in serum and plasma, making it difficult to choose good reference genes
Cancer Focus miRNA PCR Panels
miRNA and cancerNumerous studies have demonstrated links between miRNA dysregulation and various disease states, including a diverse array of human cancers. miRNAs have been shown to act as both oncogenes and tumor suppressors.The high relative stability of miRNA in common clinical source materials (e.g., FFPE blocks, plasma, urine, saliva, etc.) and the ability of miRNAs to function as molecular markers for discrete tissue types and disease states have positioned miRNA quantification as a promising new tool for a wide range of applications. miRNAs can be used as disease classifiers for different types of cancers or as biomarkers to monitor the effects of therapy on cancer prognosis and progression.Identify miRNA biomarkers or new classifiersCancer Focus miRNA PCR Panels can be used for many types of cancer miRNA profiling from samples including FFPE and biofluids. These miRNA profiles can be used to identify unique cancer signatures and help identify biomarkers or classifiers for cancer types and progression (see figuresSix types of cancers compared and miRNA expression from four types of cancer).Exosome Focus miRNA PCR Panels
How the assays were selectedAll miRNA assays in the Exosome Focus miRNA PCR Panels were selected based on a combination of experimental data from in-house studies on exosomes using our miRCURY LNA miRNA PCR System and results published in peer-reviewed journals.The assays are not a complete list targeting all miRNAs found in exosomes, but they target those miRNAs typically found (see figure Urine and CSF miRNA profiles). If missing some miRNAs is a concern, we recommend running a full miRNome study using the miRCURY LNA miRNA miRNome PCR Panels.Principle
Serum/Plasma miRNA PCR Panels
Product coverageAll 179 assays on the Serum/Plasma miRNA PCR Panels have been carefully selected based on our vast number of in-house analyses of miRNA expression in blood, serum and plasma samples, as well as on the limited number of peer-reviewed published papers available. For a complete list of miRNA assays on the panel, refer to the plate layout file. Over 1 million in-house and collaborative data points from serum/plasma samples collected from healthy and diseased individuals have been used in the selection of relevant miRNAs for the panel. This includes miRNA expression data from different stages of various cancers, neurological disorders, neurodegenerative diseases, allergies, inflammation, etc. Our database is largely based on total miRNA extracted from serum/plasma, so a particular circulating miRNA should be on the focus panel, regardless of whether it is preferentially protein bound, found in exosomes, microvesicles or otherwise compartmentalized. This results in a very comprehensive panel with broad applications.The Serum/Plasma miRNA PCR Panels are available in two formats:- 96 well, ready-to-use: two 96-well PCR plates with a total of 179 LNA miRNA primer sets commonly found in human serum/plasma, including 7 candidate reference miRNAs; compatible with most real-time PCR instruments (see table below). The product to order consists of 4 panel sets (4x two96-well plates).
- 384 well, ready-to-use: one 384-well PCR plate with two replicates of 179 LNA miRNA primer sets commonly found in human serum/plasma, including 7 candidate reference miRNAs; compatible with most real-time PCR instruments (see table below). The product to order consists of 4 panel sets (2x one 384-well plates).
- miR-103-3p, miR-191-5p and miR-423-5p: these are potential reference genes found on our miRNome PCR Panels and can be used by researchers migrating to the focus panels
- miR-93-5p and miR-425-5p: these potential reference genes are chosen because they are usually stably expressed in serum/plasma
- miR-451a and miR-23a-3p: these can be used as a control for hemolysis. If ∆Cq(miR-23a-3p – miR-451a) is >7, it may be an indication of contamination of the serum/plasma samples.
Cancer Focus miRNA PCR Panels
The miRCURY LNA miRNA Cancer Focus PCR Panel targets 84 human miRNAs related to cancer that have been shown to be important for tumorigenesis and in cancer diagnostics. These miRNAs can be used as markers for 27 different types of cancer. They have been identified from a large biobank containing thousands of FFPE samples, as well as from literature data. For a list of references of each miRNA on the panel, refer to the plate layout file.Product coverageThe Cancer Focus miRNA PCR Panel is available in:- 96-well plate format: one Cancer Focus Panel on one 96-well plate. The product to order consists of 4 panel sets (4x one 96-well plates).
- 384-well plate format: eight Cancer Focus Panels delivered in two 384-well plates (sufficient for 8 samples). These plates have been specifically designed for fast and easy manual and multi-pipetting. The product to order consists of8 panel sets (2x one 384-well plates).
- 84 miRNAs specifically linked to cancer
- 3 miRNA reference genes: miR-103-3p, miR-191-5p, miR-423-5p
- snRNA reference genes: SNORD49A, U6 snRNA, SNORD38B
- 3 Inter-plate calibrators (UniSp3 IPC)
- 5 RNA spike-ins (cel-miR-39-3p, UniSp2, UnuSp4, UniSp5 and UniSp6)
Exosome Focus miRNA PCR Panels
Key benefits of the Exosome Focus miRNA PCR Panels include:- All typically expressed miRNAs found in urine or cerebrospinal fluid samples in one PCR panel
- A highly flexible solution that allows you to focus on the miRNAs relevant to your exosome project
- Intuitive plate designer allows replacement of assays in the plates before ordering
- Sensitivity and specificity for accurate miRNA profiling
- Fast and easy, ready-to-use format enables direct sample application and a full profile in less than 3 hours
- High compatibility 96- and 384-well formats are compatible with most real-time PCR instruments
- 87 miRNA LNA PCR primer sets targeting human miRNAs expressed in human exosomes from urine
- 5 of the miRNA assays can also be used as potential internal control primers: hsa-miR-103-3p, hsa-miR-191-5p, hsa-miR-93-5p, hsa-miR-423-3p and hsa-miR-423-5p
- 5 RNA spike-in controls available for selection in the panel configurator for use as positive controls (e.g., for quality control of the RNA purification and the RT reaction)
- 3 inter-plate calibrators (3 x UniSp3 IPC)
- 87 miRNA LNA PCR primer sets targeting human miRNAs expressed in human exosomes from cerebrospinal fluid
- 3 of the miRNA assays can also be used as potential internal control primers: hsa-miR-103-3p, hsa-miR-191-5p and hsa-miR-93-5p
- 5 RNA spike-in controls available for selection in the configurator for use as positive controls (e.g., for quality control of the RNA purification and the RT reaction)
- 3 inter-plate calibrators (3 x UniSp3 IPC)
Procedure
The easy-to-follow, 3-hour protocol saves you both time and effort in the laboratory. Simply dilute the synthesized cDNA, combine it with the miRCURY LNA SYBR Green Master Mix and aliquot into the 96- or 384-well PCR plate containing the pre-aliquoted PCR Panel. The plate is now ready to run in your real-time PCR instrument.
Applications
miRNA quantification in serum, plasma, exosomes, urine and other liquid biopsies; biomarker discovery
Product Resources
Customers who bought these products also bought
- Cat No./ID:339390
RNA Spike-In Kit, For RT
Contains the UniSp2, UniSp4, and UniSp5 RNA Spike-in Template Mix and the cel-miR-39-3p RNA Spike-in Template$165.00Add To Cart - Cat No./ID:339347
miRCURY LNA SYBR Green PCR Kit (4000)
For 4000 reactions: 2X miRCURY SYBR Green Master Mix, RNase-Free Water$1,506.00Add To Cart - Cat No./ID:339340
miRCURY LNA RT Kit
For 8–64 cDNA synthesis reactions: 5x RT SYBR Green Reaction Buffer, 5x RT Probe Reaction Buffer, 10x RT Enzyme Mix, UniSp6, RNA Spike-in template, RNase-Free Water
$373.00Add To Cart - Cat No./ID:339306
miRCURY LNA miRNA PCR Assay
Contains forward and reverse primers for 200 reactions; for SYBR® Green-based detection
Select Targets - Cat No./ID:217204
miRNeasy Serum/Plasma Advanced Kit (50)
For 50 total RNA preps: 50 RNeasy MinElute Spin Columns, Collection Tubes (1.5 ml and 2 ml), RNase-Free Reagents and Buffers$461.00Add To Cart - Cat No./ID:339320
miRCURY LNA miRNA PCR Starter Kit
Two miRCURY LNA PCR Assays of your choice, spike-in control Assay (UniSp6), one candidate endogenous control assay (miR-103-3p) and all reagents for 20 reverse transcription reactions and 100 PCR amplifications; for SYBR® Green-based qPCR detection
Select Targets - Cat No./ID:339345
miRCURY LNA SYBR Green PCR Kit (200)
For 200 reactions: 2X miRCURY SYBR Green Master Mix, RNase-Free Water$180.00Add To Cart - Cat No./ID:339346
miRCURY LNA SYBR Green PCR Kit (600)
For 600 reactions: 2X miRCURY SYBR Green Master Mix, RNase-Free Water$406.00Add To Cart - Cat No./ID:37901
Qproteome Mammalian Protein Prep Kit
For approximately 100 protein preparations from cultured mammalian cells: Buffer, Reagents, Protease Inhibitor Solution, Benzonase$234.00Add To Cart - Cat No./ID:929559
QX DNA Size Marker 100bp - 2.5kb (50 µl)
DNA size marker with fragments of 100, 200, 300, 400, 500, 600, 700, 800, 1000, 1200, 1500, 2000, and 2500 bp; concentration 100 ng/µl$83.70Add To Cart - Cat No./ID:339317
miRCURY LNA miRNA Custom PCR Assay
Contains forward and reverse primers for 200 reactions; for SYBR® Green-based detection
Select Targets - Cat No./ID:208354
QuantiNova Probe RT-PCR Kit (500)
For 500 x 20 µl reactions: 3 x 1.7 ml QuantiNova Probe RT-PCR Master Mix, 100 µl QuantiNova Probe RT Mix, 100 µl Internal Control RNA, 500 µl Yellow Template Dilution Buffer, 1 ml ROX Reference Dye, 2 x 1.9 µl RNase-Free Water$663.00Add To Cart - Cat No./ID:19054
Buffer QBT (1000 ml)
1000 ml Equilibration Buffer$129.00Add To Cart - Cat No./ID:208254
QuantiNova Probe PCRKit (500)
For 500 x 20 µl reactions: 3 x 1.7 ml 2x QuantiNova Probe PCR Master Mix, 1 ml QN ROX Reference Dye, 500 µl QuantiNova Yellow Template Dilution Buffer, 1.9 ml Water$423.00Add To Cart
品牌介绍
QIAGEN是一家专业化致力于生物分子样品制备解决方案的跨国经营企业，总部位于德国。1984年，QIAGEN在德国成立，1996年在美国纽约纳斯达克上市。QIAGEN拥有超过1000项专利和认可证明，在18个国家设立了分公司，代理商服务国超过40个，在全球有超过400000的用户。QIAGEN提供的产品超过500类，包括各种试剂，耗材和自动化纯化工作站。这些产品用于样品采集，稳定，核酸或蛋白的分离，纯化和检测中，不仅广泛的应用于科研领域的各个方面，在生物技术，制药，法医研究，食品安全检测，畜牧业和分子诊断领域也得到了广泛的应用。QIAGEN产品的卓越品质和在应用中的出色表现使得其成为样品处理中标准的代名词。
联络我们
