Qiagen/miRCURY外小体试剂盒/用于从血清或血浆中分离外小体和其他细胞外小泡；包括10毫升沉淀缓冲液A、10毫升再悬浮缓冲液、200 U凝血酶和500µl凝血酶缓冲液/76603
市场价：
¥6700.00
美元价：
4020.00
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
miRCURY Exosome Kits
Print
For enrichment of exosomes and other extracellular vesicles from serum/plasma or cell/urine/CSF samples
- Excellent recovery of exosomes and other extracellular vesicles
- Easy and straightforward protocol that takes less than 2 hours
- No ultracentrifugation or phenol/chloroform steps required
- Fully compatible with the miRCURY LNA miRNA PCR System
- Suited for a variety of applications, such as miRNA or RNA profiling
miRCURY Exosome Kits enable high-quality and scalable exosome isolation with an easy protocol that does not require special laboratory equipment. The miRCURY Exosome Serum/Plasma Kit is optimized for serum and plasma samples, while the miRCURY Exosome Cell/Urine/CSF Kit is designed for processing cell-conditioned media, urine and CSF samples. Both kits provide high exosomal recovery and seamless integration with different downstream assays.
Need a quote for your research project or would you like to discuss your project with our specialist team?Just contact us!
- Buy Products
- Product Details
Buy Products
Cat No./ID:76603 miRCURY Exosome Serum/Plasma Kit $335.00 For isolation of exosomes and other extracellular vesicles from serum or plasma; includes 10 ml Precipitation Buffer A, 10 ml Resuspension Buffer, 200 U Thrombin and 500 µl Thrombin Buffer |
Cat No./ID:76743 miRCURY Exosome Cell/Urine/CSF Kit $335.00 For isolation of exosomes and other extracellular vesicles from cell culture supernatants, urine or CSF; includes 2 x 25 ml Precipitation Buffer B, 10 ml Resuspension Buffer |
miRCURY Exosome Kits are intended for molecular biology applications. These products are not intended for the diagnosis, prevention, or treatment of a disease.
Product Details
Performance
Efficient exosome isolation from serum and plasma
The miRCURY Exosome Serum/Plasma Kit is optimized forisolation of exosomes from serum and plasma samples.This kit includes thrombin for efficient isolation of exosomes from plasma. To evaluate the efficiency of exosome isolation, we quantified the fraction of exosomes found in the pellet versus the supernatant, which is normally discarded (see figure Exosome recovery from serum). We observed a very high enrichment rate of extracellular vesicles.Efficient exosome isolation from cell-conditioned media, urine and CSF
ThemiRCURY Exosome Cell/Urine/CSF Kit is optimized for isolation of exosomes from cell-conditioned media, urine and CSF samples. In studies comparing the fraction of exosomes in the pellet versus supernatant for these sample types, we observed a very high exosome enrichment rate (see figures Exosome recovery from cell-conditioned media and Exosome recovery from urine). Typically, the amount of miRNA present in urine and CSF samples is very limited in healthy individuals. Therefore, we highly recommend performing an exosome isolation of these biofluids prior to RNA isolation and PCR profiling. This allows you to use the exosome isolation kit as a way of concentrating the sample, while keeping the amount of potential PCR inhibitors to a minimum (see figure Detect more miRNAs in biofluids using exosome isolation kits).Principle
The miRCURY Exosome Kits utilize precipitation to enrich for exosomes and other extracellular vesicles. They are optimized to provide efficient isolation of exosomes from various biofluid samples, such as cell-conditioned media, urine, CSF, serum or plasma. The protocols are easy to use and take just two hours.Although they are not species specific, the kit protocols are optimized for working with human biofluids. Refer to the miRCURY Exosome Kits Handbook for recommended starting material for different sample types.
Why study exosomes?
Exosomes are cell-derived, membranous particles ranging in size from 20–120 nm, approximately the same size as viruses but considerably smaller than microvesicles (see figure The structure of an exosome). Exosomes are excreted from cells into the surrounding media and can be found in many, if not all, body fluids. Their proposed role as intercellular hormone-like messengers, together with their stability as carrier of proteins and RNA, make them ideal as biomarkers for a variety of diseases and biological processes (see figure The hypothesis of exosomal shuttling of miRNAs).Exosomes are secreted by most cell types and are formed by the fusion of multivesicular bodies with the plasma membrane. They are believed to be involved in a number of functions, including:- Immune regulation (e.g., tumor-derived exosomes may help tumors evade the immune response)
- Blood coagulation
- Cell migration
- Cell differentiation
- Cell-to-cell communication
Procedure
Samples are centrifuged to remove cells and debris, as well as cryoprecipitates for previously frozen samples. Precipitation buffer is added, and the samples are incubated for 1 hout at room temperature. Exosomes are pelleted during a 30 minute centrifugation step. After resuspension, the exosomes are ready for downstream analysis.
Applications
The miRCURY Exosome Kits are mainly developed for miRNA/RNA profiling applications.
Product Resources
Customers who bought these products also bought
- Cat No./ID:77900
Reagent UI (1 ml)
Urine inhibitor removal reagent for use with the exoRNeasy Midi Kit or exoRNeasy Maxi Kit. Contents are sufficient for use with one kit.$48.00Add To Cart - Cat No./ID:217084
miRNeasy Micro Kit (50)
For 50 total RNA preps: 50 RNeasy MinElute Spin Columns, Collection Tubes (1.5 ml and 2 ml), QIAzol Lysis Reagent, RNase-Free Reagents and Buffers$411.00Add To Cart - Cat No./ID:204076
Rotor-Gene SYBR Green PCR Kit (2000)
For 2000 x 25 µl reactions: 25 ml 2x Rotor-Gene SYBR Green PCR Master Mix, 20 ml RNase-Free Water$1,608.00Add To Cart - Cat No./ID:76064
exoEasy Maxi Kit (20)
For 20 vesicle preps: 20 exoEasy Maxi Spin Columns, Collection Tubes (50 ml), Reagents and Buffers$739.00Add To Cart - Cat No./ID:386113
Matrix Standard BT5 single cap. (5 x 25)
Matrix standard for 6-FAM, BTG, BTY, BTR, and BTO, for single-capillary analyzers$79.50Add To Cart - Cat No./ID:9018921
Adapter, 48x0.5ml Tubes
For holding 48 x 0.5 ml Tubes$724.00Add To Cart - Cat No./ID:77023
exoRNeasy Serum/Plasma Starter Kit (20)
For purification of total exosome-derived RNA from serum/plasma;includes 20 exoEasy columns (10 Maxi, 10 Midi) and 20 RNeasy MinElute spin columns$650.00Add To Cart - Cat No./ID:339347
miRCURY LNA SYBR Green PCR Kit (4000)
For 4000 reactions: 2X miRCURY SYBR Green Master Mix, RNase-Free Water$1,506.00Add To Cart - Cat No./ID:69985
Grinding Jar Set, Stainless Steel (2 x 10 ml)
2 Grinding Jars (10 ml), 2 Stainless Steel Grinding Balls (20 mm)$2,445.00Add To Cart - Cat No./ID:218160
miScript II RT Kit (12)
For 12 cDNA synthesis reactions: miScript Reverse Transcriptase Mix, 10x miScript Nucleics Mix, 5x miScript HiSpec Buffer, 5x miScript HiFlex Buffer, RNase-Free Water$141.00Add To Cart - Cat No./ID:339390
RNA Spike-In Kit, For RT
Contains the UniSp2, UniSp4, and UniSp5 RNA Spike-in Template Mix and the cel-miR-39-3p RNA Spike-in Template$165.00Add To Cart - Cat No./ID:69967
TissueLyser Single-Bead Dispenser, 7 mm
For dispensing individual beads (7 mm diameter)$261.00Add To Cart - Cat No./ID:77164
exoRNeasy Maxi Kit (50)
For purification of total exosome-derived RNA; includes 50 exoEasy Maxi columns and 50 RNeasy MinElute spin columns$2,305.00Add To Cart - Cat No./ID:339340
miRCURY LNA RT Kit
For 8–64 cDNA synthesis reactions: 5x RT SYBR Green Reaction Buffer, 5x RT Probe Reaction Buffer, 10x RT Enzyme Mix, UniSp6, RNA Spike-in template, RNase-Free Water
$373.00Add To Cart - Cat No./ID:13113-325
Ceramic Beads, 1.4 mm (325 g)
Customize your bead tubes with bulk beads for rapid and reliable biological sample lysis from a wide variety of starting materials.$264.00Add To Cart - Cat No./ID:69525
mericon DNA Bacteria Kit (100)
Fast Lysis Buffer$123.00Add To Cart - Cat No./ID:217184
miRNeasy Serum/Plasma Kit (50)
For 50 total RNA preps: 50 RNeasy MinElute Spin Columns, Collection Tubes (1.5 ml and 2 ml), QIAzol Lysis Reagent, Ce_miR-39_1 miScript Primer Assay, RNase-free Reagents and Buffers$489.00Add To Cart - Cat No./ID:217204
miRNeasy Serum/Plasma Advanced Kit (50)
For 50 total RNA preps: 50 RNeasy MinElute Spin Columns, Collection Tubes (1.5 ml and 2 ml), RNase-Free Reagents and Buffers$461.00Add To Cart - Cat No./ID:333802
QIAseq Targeted DNA IO Panel (12)
Kit containing ALL reagents (except indexes) for targeted DNA sequencing; fixed panel for 12 samples; more than 100 genesSelect Targets - Cat No./ID:301305
SuperFect Transfection Reagent (1.2 ml)
For 40 transfections in 60 mm dishes or 160 transfections in 12-well plates$328.00Add To Cart
品牌介绍
QIAGEN是一家专业化致力于生物分子样品制备解决方案的跨国经营企业，总部位于德国。1984年，QIAGEN在德国成立，1996年在美国纽约纳斯达克上市。QIAGEN拥有超过1000项专利和认可证明，在18个国家设立了分公司，代理商服务国超过40个，在全球有超过400000的用户。QIAGEN提供的产品超过500类，包括各种试剂，耗材和自动化纯化工作站。这些产品用于样品采集，稳定，核酸或蛋白的分离，纯化和检测中，不仅广泛的应用于科研领域的各个方面，在生物技术，制药，法医研究，食品安全检测，畜牧业和分子诊断领域也得到了广泛的应用。QIAGEN产品的卓越品质和在应用中的出色表现使得其成为样品处理中标准的代名词。
联络我们
