Qiagen/AdnaTest ProstateCancerPanel AR-V7/For 12 enrichments of tumor cells from whole blood and subsequent detection of prostate cancer-associated gene expression including AR-V7 expression./396132
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商品介绍
AdnaTest ProstateCancerPanel AR-V7
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For the enrichment and molecular characterization of circulating tumor cells (CTCs) from whole human blood
- Efficient isolation and detection of CTCs from whole blood
- Molecular characterization of CTCs
- High specificity and sensitivity
AdnaTest ProstateCancerPanel AR-V7 allows molecular characterization of CTCs using the Combination of Combinations Principle (COCP). AdnaTest ProstateCancerPanel AR-V7 is a highly specific immunomagnetic cell-selection system for enriching circulating tumor cells from peripheral blood.Thisallows sensitive analysis of prostate cancer-associated gene expression including AR-V7 detection in immunomagnetically enriched tumor cells by reverse transcription and PCR.
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Cat No./ID:396132 AdnaTest ProstateCancerPanel AR-V7 $2,824.00 For 12 enrichments of tumor cells from whole blood and subsequent detection of prostate cancer-associated gene expression including AR-V7 expression. |
Cat No./ID:399911 AdnaMag-S $708.00 For 8 tubes, 1.5 ml |
Cat No./ID:399921 AdnaMag-L $708.00 For 8 tubes, 15 ml |
Cat No./ID:399932 AdnaTube $77.30 12 sample tubes containing EDTA. Use only with anticoagulated blood collected in ACDA blood collection tubes from BD (Becton Dickinson, 8.5 ml) |
The AdnaTest ProstateCancerPanel AR-V7 is intended for molecular biology applications. This product is not intended for the diagnosis, prevention, or treatment of a disease.
Product Details
Performance
The AdnaTest’s successful CTC detection is based on the Combination of Combinations Principle (COCP). Each AdnaTest has a unique combination of tumor associated markers and an optimized combination of antibodies for cell selection. The combination of highly specific immunomagnetic cell selection system (using the optimized antibody combination, see “A CTC captured by three antibodies coupled to magnetic beads”) and highly sensitive RT-PCR technology (using a combination of mRNA tumor markers) provides the highest degrees of analytical specificity and sensitivity.
Principle
The AdnaTest uses a two-step process (Select and Panel) to generate results within 6 hours (see “AdnaTest ProstateCancerSelect: Immunomagnetic cell selection with multiple tumor associated antibodies” and “AdnaTest ProstateCancerPanel AR-V7: qRT-PCR of AR-V7 and various prostate cancer associated tumor markers.”). AdnaTest ProstateCancerSelect enables the immunomagnetic enrichment of tumor cells via epithelial and tumor associated antigens. Antibodies against these antigens are conjugated to magnetic beads for labeling of tumor cells in whole blood. Labeled cells are extracted by a magnetic particle concentrator (AdnaMag-L and AdnaMag-S) and are subsequently lysed (“AdnaTest ProstateCancerSelect: Immunomagnetic cell selection with multiple tumor associated antibodies.”). The cell lysate is used for further analysis with AdnaTest ProstateCancerPanel AR-V7.AdnaTest ProstateCancerPanel AR-V7 contains Oligo (dT)25 Beads for the isolation of mRNA from the lysate of pre-enriched tumor cells. Reverse transcription results in cDNA, which is subsequently used as template for preamplification and tumor cell detection and characterization by qRT-PCR. The AdnaPanel PrimerMixes allow amplification of four tumor associated antigens and two control genes. Furthermore, the kit contains PrimerMixes for amplification of an internal control and an inhibition control. See "AdnaTest ProstateCancerPanel AR-V7: qRT-PCR of AR-V7 and various prostate cancer associated tumor markers."
Procedure
Recovery
The AdnaTest ProstateCancerSelect is used for the enrichment of CTCs from whole blood in prostate cancer research. Subsequently, the AdnaTest ProstateCancerPanel AR-V7 is used for molecular characterization by analyzing gene expression of AR-V7 and further prostate cancer associated genes. In spiking experiments, 2 tumor cells in 5 ml of whole blood are detected at a recovery rate of 95%.| Recovery [%] | n | |
|---|---|---|
| 2 tumor cells spiked into 5 ml blood | 38/40 (95%) | 40 |
Specificity
Fourteen male healthy donors were analyzed with the AdnaTest ProstateCancerPanel AR-V7 to determine the rate of false positives/specificity at the given cut offs.| PSA | PSMA | ARwt | AR-V7 | |
|---|---|---|---|---|
| Cut-off | 35.00 | 35.00 | 35.00 | 35.00 |
| Positives after cut-off | 0 | 0 | 1 | 0 |
| Specificity | 100% | 100% | 93% | 100% |
qRT-PCR linearity and efficiency
Perfect linearity and efficiency of the qRT-PCR reactions for each of the genes contained in the AdnaTest ProstateCancerPanel AR-V7 was proven in a serial dilution series of standards (See “AR-V7 Panel gene dilutions”). The positive control has been used as template for standard sample generation.The resulting Ct values for this dilution series were clearly linear, correlated to the copy numbers for each gene at each dilution step (See “AR-V7 Panel gene dilutions”) getting down to ~500 copies. The qRT-PCR efficiency of all the tumor cell related genes was higher than 90% and the corresponding amplification factors ranged above 1.9 which all in all can be taken as a prove for very effective and sensitive qRT-PCR design (see the table below).| PSA | PSMA | AR | AR-V7 | |
|---|---|---|---|---|
| Slope over the range | -3.55 | -3.58 | -3.56 | -3.48 |
| Efficiency: -1+10(-1/slope) | 91% | 90% | 91% | 94% |
| Amplification factor: 10^(1/-slope) | 1.91 | 1.90 | 1.91 | 1.94 |
Field test results
The AdnaTest ProstateCancerPanel AR-V7 workflow was tested in 12 castration-resistant prostate cancer (CRPC) samples. See "Gene profile in clinical samples."Applications
- Isolation of CTCs from whole human blood
- Molecular characterization of CTCs
- Biomarker discovery
- mRNA profiling
- Liquid biopsy
- Cancer research
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品牌介绍
QIAGEN是一家专业化致力于生物分子样品制备解决方案的跨国经营企业，总部位于德国。1984年，QIAGEN在德国成立，1996年在美国纽约纳斯达克上市。QIAGEN拥有超过1000项专利和认可证明，在18个国家设立了分公司，代理商服务国超过40个，在全球有超过400000的用户。QIAGEN提供的产品超过500类，包括各种试剂，耗材和自动化纯化工作站。这些产品用于样品采集，稳定，核酸或蛋白的分离，纯化和检测中，不仅广泛的应用于科研领域的各个方面，在生物技术，制药，法医研究，食品安全检测，畜牧业和分子诊断领域也得到了广泛的应用。QIAGEN产品的卓越品质和在应用中的出色表现使得其成为样品处理中标准的代名词。
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