Qiagen/AdnaTest BreastCancer/RT-PCR Kit for detection of breast cancer-associated gene expression in enriched tumor cells/395012
市场价：
¥18480.00
美元价：
11088.00
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
AdnaTest BreastCancer
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For the enrichment and molecular characterization of circulating tumor cells (CTCs) from whole human blood
- Efficient isolation and detection of CTCs from whole blood
- Molecular characterization of CTCs
- High specificity and sensitivity
- Processing of multiple samples in parallel
AdnaTest BreastCancer allows molecular characterization of CTCs by combining AdnaTest BreastCancerSelect with AdnaTest BreastCancerDetect using the Combination of Combinations Principle (COCP). AdnaTest BreastCancerSelect is a highly specific immunomagnetic cell-selection system for enriching circulating tumor cells from peripheral blood. AdnaTest BreastCancerDetect allows sensitive analysis of breast cancer-associated gene expression in immunomagnetically enriched tumor cells by reverse transcription and PCR.
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- Product Details
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Cat No./ID:395012 AdnaTest BreastCancerSelect $924.00 RT-PCR Kit for detection of breast cancer-associated gene expression in enriched tumor cells |
Cat No./ID:396012 AdnaTest BreastCancerDetect $924.00 RT-PCR Kit for detection of breast cancer-associated gene expression in enriched tumor cells. |
Cat No./ID:399911 AdnaMag-S $708.00 For 8 tubes, 1.5 ml |
Cat No./ID:399921 AdnaMag-L $708.00 For 8 tubes, 15 ml |
Cat No./ID:399932 AdnaTube $77.30 12 sample tubes containing EDTA. Use only with anticoagulated blood collected in ACDA blood collection tubes from BD (Becton Dickinson, 8.5 ml) |
AdnaTest BreastCancer are intended for molecular biology applications. These products are not intended for the diagnosis, prevention, or treatment of a disease.
Product Details
Performance
AdnaTest BreastCancer is a highly specific immunomagnetic cell selection system for enrichingcirculating tumor cells from peripheral blood and includesa highly sensitive RT-PCR for detecting breast cancer-specific mRNA markers.
Principle
AdnaTest technology uses an optimized combination of antibodies for cell selection in 5 ml whole blood and a combination of tumor-associated markers for the detection of tumor cells: EpCAM, CA15-3 (Muc1), presence of Her2 receptors (optional detection of estrogen and progesterone receptors by AdnaTest ER / PR Detect).
Procedure
The AdnaTestuses a two-step process (Select and Detect) to generate resultswithin 5 hours (see figures “AdnaTest workflow”). AdnaTest BreastCancerSelect enables the immunomagnetic enrichment of tumor cells from whole blood via epithelial- and tumor-associated antigens. Antibodies against epithelial- and tumor-associated antigens are conjugated to magnetic beads for labeling of tumor cells. Labeled cells are extracted by a magnetic particle concentrator (AdnaMag-L and AdnaMag-S), lysedand mRNA is then purified from these lysates (see figures “AdnaTest Select – Immunomagnetic cell selection with multiple tumor-associated antibodies labeled to magnetic beads”).In the second step, the isolated mRNA is transcribed into cDNA and can be amplified using multiplex PCR (see figures “AdnaTest Detect – Multiplex PCR of various cancer-associated tumor markers”). AdnaTest BreastCancerDetect contains Oligo (dT) 25-coated beads for the isolation of mRNA from the lysate of pre-enriched tumor cells. Reverse transcription results in cDNAthatcan subsequently be used as template for tumor cell detection and characterization by multiplex PCR. Multiplex PCR provides tumor-associated gene expression profiles for a variety of relevant tumor markers to ensure that the selected cells are cancer cells. The PrimerMix BreastDetect allows amplification of3 tumor-associated genes and1 control gene (see table below). The primers generate fragments of the following sizes:
Importantly, the AdnaTest is an open platform that allows the profiling of additional custom selected mRNA targetsfor comphrehensive biomarker discovery.
| PrimerMix BreastDetect | |
| Target gene | PCR fragment size (bp) |
| GA733-2 | 395 |
| Muc-1 | 299 |
| Her-2 | 265 |
| Actin (internal PCR control) | 120 |
Applications
- Isolation of CTCs from whole human blood
- Molecular characterization of CTCs
- Biomarker discovery
- mRNA profiling
- Liquid biopsy
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品牌介绍
QIAGEN是一家专业化致力于生物分子样品制备解决方案的跨国经营企业，总部位于德国。1984年，QIAGEN在德国成立，1996年在美国纽约纳斯达克上市。QIAGEN拥有超过1000项专利和认可证明，在18个国家设立了分公司，代理商服务国超过40个，在全球有超过400000的用户。QIAGEN提供的产品超过500类，包括各种试剂，耗材和自动化纯化工作站。这些产品用于样品采集，稳定，核酸或蛋白的分离，纯化和检测中，不仅广泛的应用于科研领域的各个方面，在生物技术，制药，法医研究，食品安全检测，畜牧业和分子诊断领域也得到了广泛的应用。QIAGEN产品的卓越品质和在应用中的出色表现使得其成为样品处理中标准的代名词。
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