Effectene Transfection Reagent
- High efficiency in the presence of serum
- Efficient transfection with low DNA amounts
- Far lower cytotoxicity and gentler than many alternatives
- Suitable for high-throughput screening
- Buy Products
- Product Details
- Specifications
Buy Products
Cat No./ID:301425 Effectene Transfection Reagent (1 ml) $357.00 1 ml Effectene Reagent, Enhancer, Buffer; for 40 transfections in 60 mm dishes or 160 transfections in 12-well plates |
Cat No./ID:301427 Effectene Transfection Reagent (4 x 1 ml) $1,232.00 4 x 1 ml Effectene Reagent, Enhancer, Buffer; for 160 transfections in 60 mm dishes or 640 transfections in 12-well plates |
Product Details
Higher transfection efficiencies of plasmid DNA are achieved with Effectene Transfection Reagent than with other reagents when used with the recommended procedures (see figure "High transfection efficiencies using Effectene Reagent"). Effectene Transfection Reagent is suitable for transfecting sensitive cell lines with oligonucleotides (see figure "Transfection of oligonucleotides using Effectene Reagent") and is particularly effective for primary cells (see figure "40% transfection efficiency in primary cells"). Many cell lines and primary cells have been successfully transfected using Effectene Reagent; cell type-specific transfection protocols are available. Cytotoxicity is minimal because transfection with Effectene Reagent can be performed in the presence of serum and requires low amounts of DNA (see figure "Serum and DNA quantity vs. transfection efficiency").
The application of recombinant DNA technology to fields such as drug discovery and development has led to an increased need for high-throughput transfection. Transfection using Effectene Transfection Reagent requires low amounts of DNA and minimal handling. In addition, removal of transfection complexes is not required, making this reagent highly suitable for high-throughput screening. Effectene Transfection Reagent is available in bulk quantities.
Effectene Transfection Reagent is an innovative non-liposomal lipid formulation that is used in conjunction with a special DNA-condensing enhancer and optimized buffer to achieve high transfection efficiencies. The enhancer first condenses the DNA molecules and Effectene Reagent subsequently coats them with cationic lipids providing a particularly efficient way of transferring DNA into eukaryotic cells. This feature ensures excellent reproducibility of transfection complex formation.
The Effectene procedure has two steps. DNA is first mixed with Enhancer and a buffer that provides optimal salt conditions for efficient DNA condensation. This step requires just 2–5 minutes. Effectene Reagent is then added and the mixture is incubated for 5–10 minutes to allow Effectene–DNA complexes to form. The complexes are mixed with growth medium (which can contain serum and antibiotics), and added directly to the cells. The cells are then incubated until harvested and analyzed for gene expression (see flowchart "Effectene transfection procedure").
Effectene Transfection Reagent is suitable for transient and stable transfection of a broad range of cell types.
Specifications
Features | Specifications |
| Applications | Plasmid transfection, protein overexpression, reporter studies |
| Cell type | Eukaryotic cells (primary cells and sensitive cells) |
| Controls | Not included |
| Features | Non-liposomal lipid formulation, minimal cytotoxicity |
| Nucleic acid | DNA |
| Number of possible transfections | 160 transfections in 12-well plates / 1 ml reagent |
| Technology | Non-liposomal lipid formulation in conjonction with a DNA-condensing enhancer |
| Transfection type | Transient and stable transfection |
Product Resources
Customers who bought these products also bought
- Cat No./ID:76064
exoEasy Maxi Kit (20)
For 20 vesicle preps: 20 exoEasy Maxi Spin Columns, Collection Tubes (50 ml), Reagents and Buffers$739.00Add To Cart - Cat No./ID:301705
HiPerFect Transfection Reagent (1 ml)
HiPerFect Transfection Reagent for up to 333 transfections in 24-well plates or up to 1333 transfections in 96-well plates$404.00Add To Cart - Cat No./ID:10043
QIAGEN-tip 100 (25)
25 columns$300.00Add To Cart - Cat No./ID:28706X4
QIAquick Gel Extraction Kit (1000)
For gel extraction or cleanup of 1000 reactions: 1000 QIAquick Spin Columns, Buffers, Collection Tubes (2 ml)$1,879.00Add To Cart - Cat No./ID:30310
NTA Agarose (25 ml)
25 ml uncharged resin (max. pressure 2.8 psi)$329.00Add To Cart - Cat No./ID:301005
Attractene Transfection Reagent (1 ml)
Attractene Transfection Reagent for up to 660 transfections in 24-well plates$422.00Add To Cart - Cat No./ID:12165
QIAGEN Plasmid Maxi Kit (100)
100 QIAGEN-tip 500, Reagents, Buffers$2,240.00Add To Cart - Cat No./ID:36113
Ni-NTA Magnetic Agarose Beads (6 x 1 ml)
6 x 1 ml nickel-charged magnetic agarose beads (5%suspension)$703.00Add To Cart - Cat No./ID:34964
Polypropylene Columns (5 ml)
50/pack, 5 ml capacity$189.00Add To Cart - Cat No./ID:301305
SuperFect Transfection Reagent (1.2 ml)
For 40 transfections in 60 mm dishes or 160 transfections in 12-well plates$328.00Add To Cart - Cat No./ID:67563
MagAttract HMW DNA Kit (48)
For 48 DNA preps: MagAttract Suspension G, Buffer ATL, Buffer AL, Buffer MB, Buffer MW1, Buffer PE, Proteinase K, RNase A, Buffer AE, Nuclease-Free Water$339.00Add To Cart - Cat No./ID:77023
exoRNeasy Serum/Plasma Starter Kit (20)
For purification of total exosome-derived RNA from serum/plasma;includes 20 exoEasy columns (10 Maxi, 10 Midi) and 20 RNeasy MinElute spin columns$650.00Add To Cart - Cat No./ID:204074
Rotor-Gene SYBR Green PCR Kit (400)
For 400 x 25 µl reactions: 3 x 1.7 ml 2x Rotor-Gene SYBR Green PCR Master Mix, 2 x 2 ml RNase-Free Water$471.00Add To Cart - Cat No./ID:208054
QuantiNova SYBR Green PCR Kit (500)
For 500 x 20 µl reactions: 3 x 1.7 ml 2x QuantiNova SYBR Green PCR Master Mix, 500 µl QuantiNova Yellow Template Dilution Buffer, 1 ml QN ROX Reference Dye, 1.9 ml Water$429.00Add To Cart - Cat No./ID:399911
AdnaMag-S
For 8 tubes, 1.5 ml$708.00Add To Cart - Cat No./ID:30210
Ni-NTA Agarose (25 ml)
25 ml nickel-charged resin (max. pressure: 2.8 psi)$323.00Add To Cart - Cat No./ID:34924
Polypropylene Columns (1 ml)
50/pack, 1 ml capacity$147.00Add To Cart - Cat No./ID:12362
EndoFree Plasmid Maxi Kit (10)
10 QIAGEN-tip 500, Reagents, 10 QIAfilter Maxi Cartridges, Endotoxin-free Buffers$355.00Add To Cart - Cat No./ID:12843
CompactPrep Plasmid Midi Kit (25)
25 CompactPrep Midi Columns, Extender tubes, Reagents, Buffers, LyseBlue$290.00Add To Cart - Cat No./ID:12143
QIAGEN Plasmid Midi Kit (25)
25 QIAGEN-tip 100, Reagents, Buffers$303.00Add To Cart
