PolyFect Transfection Reagent
- Optimized transfection using cell-specific protocols
- Fast procedure and easy handling
- High cell viability, low cytotoxicity
PolyFect Transfection Reagent is based on activated dendrimer technology optimized for the transfection of DNA into COS-7, NIH/3T3, HeLa, 293, and CHO cells.
- Buy Products
- Product Details
- Specifications
Buy Products
Cat No./ID:301105 PolyFect Transfection Reagent (1 ml) $178.00 For 25–65 transfections in 60 mm dishes or 50–100 transfections in 6-well plates. |
Cat No./ID:301107 PolyFect Transfection Reagent (4 x 1 ml) $641.00 For 100–260 transfections in 60 mm dishes or 200–400 transfections in 6-well plates. |
Cat No./ID:301108 PolyFect Transfection Reagent (100 ml) $13,183.00 For 2500–6500 transfections in 60 mm dishes or 5000–10,000 transfections in 6-well plates. |
Product Details
PolyFect Reagent consistently delivers high transfection efficiencies (see figures "High transfection efficiencies in CHO and 293 cells" and "PolyFect Reagent with HeLa cells"). In contrast to many liposomal reagents, PolyFect Reagent enables transfection in the presence of serum without lowering of transfection efficiency.
PolyFect Transfection Reagent is a solution of specifically designed activated-dendrimers. The reagent consists of dendrimer molecules of a defined spherical architecture with branches radiating from a central core (see figure "Activated dendrimer"). The branches terminate at charged amino groups, which can interact with negatively charged phosphate groups of nucleic acids. PolyFect Reagent assembles DNA into compact structures (see figure "PolyFect–DNA interaction"), that bind to the cell surface and are taken into the cell by nonspecific endocytosis. The reagent buffers the pH of the endosome, leading to pH inhibitition of endosomal nucleases, which ensures stability of PolyFect–DNA complexes.
PolyFect Transfection Reagent is provided as a ready-to-use solution. The transfection procedure (see flowchart "PolyFect transfection procedure") is fast and simple — just add PolyFect Reagent to the DNA solution, mix, incubate for 5–10 minutes, add growth medium (which can contain serum and antibiotics), and pipet the PolyFect–DNA complexes onto the cells. The cells are then incubated for gene expression. No post-transfection removal of complexes or medium change/addition is necessary, making the procedure fast and easy.
High-throughput transfection
Transfection using PolyFect Reagent requires minimal handling and removal of transfection complexes is not required, making this reagent highly suitable for high-throughput screening. PolyFect Reagent provides outstanding transfection efficiencies, excellent reproducibility, and minimal cytotoxicity in high-throughput transfection, and is available in bulk quantities. For optimized protocols for transfection of COS-7, NIH/3T3, HeLa, HeLa-S3, 293, and CHO cells in 96-well plates, please contact QIAGEN Technical Services.
PolyFect Transfection Reagent is highly suited for efficient transfection of standard cell lines in:
- Studies on gene expression and function
- Drug discovery and development studies
Specifications
Features | Specifications |
| Applications | Plasmid transfection, protein overexpression |
| Cell type | COS-7, NIH/3T3, HeLa, 293, CHO cells |
| Controls | Not included |
| Features | Optimized protocols for COS-7, NIH/3T3, HeLa 293, and CHO cells. Transfection in the presence of serum |
| Nucleic acid | DNA |
| Number of possible transfections | Up to 50–100 transfections in 6-well plates / 1 ml reagent |
| Technology | Activated dendrimers |
| Transfection type | Transient transfection, stable transfection |
Product Resources
Customers who bought these products also bought
- Cat No./ID:239135
GelPilot Mid Range Ladder (100)
Ready-to-use DNA marker; 600 µl (100 lanes), 6 fragments: 100–2000 bp$171.00Add To Cart - Cat No./ID:301305
SuperFect Transfection Reagent (1.2 ml)
For 40 transfections in 60 mm dishes or 160 transfections in 12-well plates$328.00Add To Cart - Cat No./ID:12165
QIAGEN Plasmid Maxi Kit (100)
100 QIAGEN-tip 500, Reagents, Buffers$2,240.00Add To Cart - Cat No./ID:1022064
GFP-22 siRNA (5 nmol)
Control siRNA targeting green fluorescent protein$182.00Add To Cart - Cat No./ID:381215
Investigator HDplex Kit (100)
Primer Mix, Reaction Mix, Multi Taq2 Polymerase, Control DNA, DNA Size Standard 550 (BTO), Allelic Ladder HDplex, and Nuclease-Free WaterAdd To Cart - Cat No./ID:34650
RGS·His Antibody, BSA-free (100 μg)
100 μg mouse anti·RGS(H)4 (lyophilized, BSA-free, for 1000ml working solution)$742.00Add To Cart - Cat No./ID:386526
Investigator STR GO!PunchBuffer (200)
Lysis buffer for 200 samples of epithelial cells on paper$340.00Add To Cart - Cat No./ID:239125
GelPilot Wide Range Ladder (100)
Ready-to-use DNA marker; 600 µl (100 lanes), 7 fragments: 200–4500 bp$148.00Add To Cart - Cat No./ID:180025
QIAseq cfDNA All-in-One Kit (96)
For 96 reactions on Illumina® sequencers: QIAamp Mini Columns, tubes, reagents and buffers for cfDNA extraction for NGS. Enzymes and buffers for cfDNA library prep, Illumina Adapter Plate 96-plex, Illumina Library Amplification Primer and PCR Master Mix$4,793.00Add To Cart - Cat No./ID:301707
HiPerFect Transfection Reagent (4 x 1 ml)
HiPerFect Transfection Reagent for up to 1332 transfections in 24-well plates or up to 5333 transfections in 96-well plates$1,422.00Add To Cart - Cat No./ID:47044
NoviPure Microbial Protein Kit (50)
For the isolation of total cellular proteins from microbial cultures$396.00Add To Cart - Cat No./ID:239035
GelPilot 100 bp Ladder (100)
Ready-to-use DNA marker; 600 µl (100 lanes), 6 fragments: 100–600 bp$156.00Add To Cart - Cat No./ID:386035
DNA Size Standard 24plex (BTO) 100
DNA Size Standard 24plex (BTO) for 100 reactions
$130.00Add To Cart - Cat No./ID:28181
QIAquick 96 PCR Purification Kit (4)
For purification of 4 x 96 PCR reactions: 4 QIAquick 96 Plates, Buffers, Collection Microtubes (1.2 ml), Caps$868.00Add To Cart - Cat No./ID:382615
Investigator 26plex QS Kit (100)
Primer Mix, Fast Reaction Mix 3.0includingTaqDNA Polymerase, Control DNA, allelic ladder 26plex, and nuclease-free water
$2,344.00Add To Cart - Cat No./ID:929500
QX Intensity Calibration Marker (1 ml)
1 ml QX Intensity Calibration Marker$81.40Add To Cart - Cat No./ID:382428
Investigator 24plex GO! Kit (1000)
Primer Mix, Fast Reaction Mix 2.0including Taq DNA polymerase, Control DNA, allelic ladder 24plex, DNA size standard 24plex (BTO)$17,983.00Add To Cart - Cat No./ID:330529
RT² SYBR Green ROX qPCR Mastermix (25ml)
Contains 1 bottle of 25 ml mastermix: for 20 x 96-well RT² PCR Arrays, 12 x 384-well RT² PCR Arrays or 2000 x 25 µl reactionsor 5000 x 10µl reactions$1,585.00Add To Cart - Cat No./ID:231124
QIAGEN PCR Cloning Kit (40)
For 40 reactions: 2x Ligation Master Mix (200 µl), pDrive Cloning Vector (2.0 µg), Distilled water (1.7 ml)$420.00Add To Cart - Cat No./ID:158745
Gentra Puregene Cell Kit (2 x 108)
For (2 x 108) cells: RNase A Solution and Reagents$120.00Add To Cart
