Qiagen/TransMessenger Transfection Reagent/For 60 transfections in 6-well plates or 80 transfections in 12-well plates/301525
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¥5020.00
美元价：
3012.00
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3392242852
电话号码：
4000-520-616
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info@ebiomall.com
商品介绍
TransMessenger Transfection Reagent
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For efficient mRNA transfection
- Highly efficient transfection
- Reproducible results ensured by stringent quality control
- Efficient transfection of primary neuronalcells
TransMessenger Transfection Reagent is a ready-to-use, lipid-based reagent for RNA transfection of eukaryotic cells. Transfection of cells with RNA rather than DNA offers new possibilities for transfection experiments. Cell lines successfully transfected with TransMessenger Reagent include 293T, Jurkat, and Vero. This product can also be used for RNA transfection of primary human fibroblasts, rat cardiomyocytes, and rat neuronal cells.
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Cat No./ID:301525 TransMessenger Transfection Reagent (0.5ml) $251.00 For 60 transfections in 6-well plates or 80 transfections in 12-well plates |
The TransMessenger Transfection Reagent is intended for molecular biology applications. This product is not intended for the diagnosis, prevention, or treatment of a disease.
Product Details
Performance
TransMessenger Reagent provides a fast and easy procedure and high transfection efficiencies (see figure "TransMessenger Reagent with HeLa cells"). Since the amount of RNA is a critical factor for successful transfection, we recommend optimizing the amounts of RNA and TransMessenger Transfection Reagent for every cell type–RNA combination (see figure "Amount of RNA and TransMessenger Reagent vs. transfection efficiency"). To facilitate this, the reagent is provided with guidelines for optimization together with starting points for optimization in different cell-culture formats. TransMessenger Transfection Reagent is highly suited for use in functional cardiac cell studies using neonatal rat ventricular cardiac cells.Cardiomyocytes from cryopreserved, dissociated neonatal rat ventricular cardiac cells (R-CM-561 [QBMCellScience.com]) display excellent viability, pharmacology, morphology, and connectivity, as well as contractile and electrical activity necessary for use in functional screening (see video Transfection control: Functional Cardiomyocytes Beating). To evaluate the suitability of using TransMessenger Transfection Reagent in functional cardiac cell studies, cells were transfected with a GFP-expression vector 4 hours after plating, and were fixed 5 days post-transfection. Use of TransMessenger Transfection Reagent resulted in an excellent, 60% survival rate post-transfection with a transfection efficiency of 10–13%. All cardiomyocytes displayed beating post-transfection (sees videos GPF-Positive Cardiomyocytes Beating Post-Transfection and Functional Cardiomyocytes Beating Post-Transfection), indicating functional cells.The number of transfected cardiomyocyte verses non-cardiomyocyte cells was evaluated and results demonstrate that a high number of cardiomyocyte and non-cardiomyocyte cells were transfected.Equivalent morphologies between transfected and non-transfected cellswere also observed (see figure Cardiac cell studies).
Principle
TransMessenger Transfection Reagent is the first reagent specifically developed for transfection of cells with RNA. The reagent is a lipid-based formulation that is used in conjunction with a specific RNA-condensing enhancer and an optimized buffer. RNA molecules are condensed by the enhancer and then coated by TransMessenger Reagent for efficient transfer into eukaryotic cells. Strict quality control is performed to test for absence of RNase activity, lot-to-lot consistency, and low endotoxin levels (≤10 EU/ml). Our rigorous standards eliminate reagent variables that can adversely affect the efficiency of RNA transfection.
Procedure
All TransMessenger Reagent components are provided as ready-to-use solutions. To generate TransMessenger–RNA transfection complexes, simply mix your RNA with Enhancer R and Buffer EC-R and incubate for 5 minutes at room temperature, then add TransMessenger Reagent and incubate for a further 5–10 minutes. The complexes are mixed with medium and added directly to the cells. Following a 3 hour incubation, the medium is changed and the cells are incubated until they are ready for analysis.Optimal transfection results are achieved using high-purity RNA that is free of DNA, proteins, and other contaminants. RNA purified with RNeasy and Oligotex mRNA Kits is highly recommended.
Applications
Transfection of cells with RNA rather than DNA offers new possibilities for transfection experiments. Transfected RNA sequences are expressed in the absence of transcription, and in a promoter-independent manner. In addition, protein expression usually occurs sooner following transfection of RNA rather than DNA.
RNA transfection with TransMessenger Transfection Reagent can be used for:
- Studies of cells not efficiently transfected with plasmid
- DNA Direct studies of RNA function
Specifications
Features | Specifications |
| Applications | Direct studies of RNA function |
| Cell type | Eukaryotic cells |
| Controls | Not included |
| Features | Transfection with RNA. Efficient transfection of neuronal cells. |
| Nucleic acid | RNA |
| Number of possible transfections | 80 transfections in 12-well plates / 0,5 ml reagent |
| Technology | Lipid-based formulation in conjunction with a RNA-condensing enhancer |
| Transfection type | Transient transfection, co-transfection |
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QIAGEN是一家专业化致力于生物分子样品制备解决方案的跨国经营企业，总部位于德国。1984年，QIAGEN在德国成立，1996年在美国纽约纳斯达克上市。QIAGEN拥有超过1000项专利和认可证明，在18个国家设立了分公司，代理商服务国超过40个，在全球有超过400000的用户。QIAGEN提供的产品超过500类，包括各种试剂，耗材和自动化纯化工作站。这些产品用于样品采集，稳定，核酸或蛋白的分离，纯化和检测中，不仅广泛的应用于科研领域的各个方面，在生物技术，制药，法医研究，食品安全检测，畜牧业和分子诊断领域也得到了广泛的应用。QIAGEN产品的卓越品质和在应用中的出色表现使得其成为样品处理中标准的代名词。
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