Qiagen/Penta和middot；His HRP结合物试剂盒/125μl Penta·His HRP结合物，5 g封闭试剂，50 ml封闭试剂缓冲液（10倍浓度）/34460
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¥10960.00
美元价：
6576.00
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3392242852
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4000-520-616
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商品介绍
Penta·His HRP Conjugate Kit
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For sensitive, direct detection of His-tagged proteins
- High-sensitivity and high-speed detection of His-tagged proteins
- No need for secondary antibody
- Complete kit with optimized blocking reagent
The Penta·His HRP Conjugate Kit is for sensitive detection of recombinant proteins carrying His tags without the need for secondary antibodies. It consists of Anti·His antibodies conjugated to horseradish peroxidase (HRP). In detection procedures, the Anti·His antibody binds to a protein"s His tag and the complex is localized using chemiluminescent or chromogenic substrates that react with HRP.
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Cat No./ID:34460 Penta·His HRP Conjugate Kit $548.00 125 μl Penta·His HRP Conjugate, 5 g Blocking Reagent, 50 ml Blocking Reagent Buffer (10x concentrate) |
The Penta·His HRP Conjugate Kit is intended for molecular biology applications. This product is not intended for the diagnosis, prevention, or treatment of a disease.
Product Details
Performance
| Property | Value |
|---|---|
| Detection: | N-terminal, C-terminal, and internal 6xHis tags |
| Sensitivity in dot blots:* | 10 pg |
| Sensitivity in western blots:* | 50 pg |
| Antibody Isotype: | Mouse IgG1 |
| Cross-reactivity: | Negligible with crude E. coli, yeast, mammalian, or insect cell lysates |
| Form: | Stabilized stock solution |
| Working solution: | 125–250 ml |
Principle
The Penta·His HRP Conjugate Kit consists of the QIAexpress Anti·His Antibodies chemically coupled to the reporter enzyme horseradish peroxidase (HRP). It can be used for highly specific and sensitive, direct detection of any recombinant protein that has an accessible His tag. The Anti·His HRP Conjugate Kits eliminate the need for secondary antibodies, saving time in immunodetection procedures.
Procedure
The QIAexpress, Detection and Assay Handbook, supplied with all QIAexpress detection and assay products, contains detailed example protocols and guidelines for designing and performing assays.
Applications
The Penta·His HRP Conjugate Kit can be used with chemiluminescent or chromogenic detection substrates in:
- Western, dot, and colony blotting procedures
- ELISA and other immunoassay procedures
Specifications
Features | Specifications |
| Conjugated enzyme | HRP |
| Detection | Secondary antibody required |
| Epitope detected | HHHHH |
| Substrate for blot detection | 4C1N |
| Substrates for assay procedure | OPD |
| Tag | His tag |
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品牌介绍
QIAGEN是一家专业化致力于生物分子样品制备解决方案的跨国经营企业，总部位于德国。1984年，QIAGEN在德国成立，1996年在美国纽约纳斯达克上市。QIAGEN拥有超过1000项专利和认可证明，在18个国家设立了分公司，代理商服务国超过40个，在全球有超过400000的用户。QIAGEN提供的产品超过500类，包括各种试剂，耗材和自动化纯化工作站。这些产品用于样品采集，稳定，核酸或蛋白的分离，纯化和检测中，不仅广泛的应用于科研领域的各个方面，在生物技术，制药，法医研究，食品安全检测，畜牧业和分子诊断领域也得到了广泛的应用。QIAGEN产品的卓越品质和在应用中的出色表现使得其成为样品处理中标准的代名词。
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