Qiagen/Penta·His Antibody, BSA-free/100 μg mouse anti-(H)5 (lyophilized, BSA-free, for 1000 ml working solution)/34660
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¥16840.00
美元价：
10104.00
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3392242852
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4000-520-616
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info@ebiomall.com
商品介绍
Penta·His Antibody, BSA-free
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For sensitive detection of His-tagged proteins
- Detection of C-terminal, N-terminal, and internal His tags
- Highly sensitive and specific detection of HHHHH epitopes
Monoclonal mouse Anti·His Antibodies are used to detect recombinant proteins carrying His tags. Each of the three variants detects a different His tag epitope. Like all QIAGEN mouse monoclonal antibodies, they are prepared under serum-free conditions, which guarantees the absence of viruses, mycoplasma, and contaminating immunoglobulins.
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Cat No./ID:34660 Penta·His Antibody, BSA-free (100 μg) $842.00 100 μg mouse anti-(H)5 (lyophilized, BSA-free, for 1000 ml working solution) |
The Penta·His Antibody, BSA-freeis intended for molecular biology applications. This product is not intended for the diagnosis, prevention, or treatment of a disease.
Product Details
Performance
| Property | Value |
|---|---|
| Detection: | N-terminal, C-terminal, and internal 6xHis tags |
| Sensitivity in dot blots:* | 0.5 ng |
| Isotype: | Mouse IgG1 |
| Cross-reactivity: | Negligible with crude E. coli, yeast, mammalian, or insect cell lysates |
| Form: | Lyophilized |
| Working solution: | 1000 ml |
Many different proteins have been detected, via their His tags, using the QIAexpress Anti·His Antibodies (see table). For the sensitivity of the three Anti·His Antibodies in detecting this panel of proteins see the figure Sensitivity of QIAexpress Anti·His Antibodies)
| Protein | Tag location | Sequence |
|---|---|---|
| A DHFR | N-terminus | MRGSHHHHHHGSVRPLNCIV... |
| B DHFR | C-terminus | ...VYEKKDGSRSHHHHHH |
| C Thioredoxin | N-terminus | MRGSHHHHHHGSSDKIIHLT... |
| D TNFα | N-terminus | MRHHHHHHQSTESMIRDV... |
| E TFIIAγ | N-terminus | MGSSHHHHHHSSGLVPRGSH... |
| F Chaperonin | C-terminus | ...AKIAKDGSRSHHHHHH |
| G DNA Polymerase | C-terminus | ...EDWLSAKERSHHHHHH |
| H TFIIAαβ | Internal | ...QQQHQPQQQQHHHHHHQQAQPQQTVP... |
For other experiments with Anti·His Antibodies see figures Identification of positive expression clones by colony blotting and His-tagged thioredoxin detected by Penta·His Antibodies in yeast cells
Principle
All QIAexpress mouse monoclonal antibodies are produced under conditions that guarantee the highest purity and activity. Hybridoma cell culture is carried out under serum-free conditions, ensuring that the preparations are free of viruses, mycoplasma, and contaminating immunoglobulins. Purification by adsorption chromatography is performed entirely at physiological pH, yielding preparations with the highest proportion of native antibody. See figure Immunohistochemistry with Penta·His Antibodies.
Applications
QIAexpress Detection Systems allow efficient detection of His-tagged proteins and are ideal for many applications, including:
- Colony, dot, and western blotting procedures
- Screening for positive expression clones
- Monitoring expression levels and stability of His-tagged proteins
- Immunoprecipitation and ELISA
Specifications
Features | Specifications |
| Detection | Secondary antibody required |
| Epitope detected | HHHHH |
| Sensitivity in dot blots (chemiluminescent detection) | 10 pg |
| Sensitivity in dot blots (chromogenic detection) | 0.5 ng |
| Sensitivity in Western blots (chemiluminescent detection) | 50 pg |
| Sensitivity in Western blots (chromogenic detection) | 2 ng |
| Substrate for blot detection | Dependent on secondary antibody |
| Substrates for assay procedure | Dependent on secondary antibody |
| Tag | His-tag |
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品牌介绍
QIAGEN是一家专业化致力于生物分子样品制备解决方案的跨国经营企业，总部位于德国。1984年，QIAGEN在德国成立，1996年在美国纽约纳斯达克上市。QIAGEN拥有超过1000项专利和认可证明，在18个国家设立了分公司，代理商服务国超过40个，在全球有超过400000的用户。QIAGEN提供的产品超过500类，包括各种试剂，耗材和自动化纯化工作站。这些产品用于样品采集，稳定，核酸或蛋白的分离，纯化和检测中，不仅广泛的应用于科研领域的各个方面，在生物技术，制药，法医研究，食品安全检测，畜牧业和分子诊断领域也得到了广泛的应用。QIAGEN产品的卓越品质和在应用中的出色表现使得其成为样品处理中标准的代名词。
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