Tetra·His Antibody, BSA-free
For sensitive detection of His-tagged proteins with HHHH epitopes
- Detection of C-terminal, N-terminal, and internal His tags
- Highly specific and sensitive detection
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- Product Details
- Specifications
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Cat No./ID:34670 Tetra·His Antibody, BSA-free (100 µg) $842.00 100 µg mouse anti-(H)4 (lyophilized, BSA-free, for 1000 ml working solution) |
Product Details
The sensitivity of Tetra·His Antibodyin western blots is up to 2 ng, and up to 0.5 ng in dot blots (see figure "Sensitivity of Anti·His Antibodies"), when carrying out detection using a chromogenic substrate (sensitivity may vary with different proteins).
| Protein | Tag location | Sequence | |
|---|---|---|---|
| A | DHFR | N-terminus | MRGSHHHHHHGSVRPLNCIV.......... |
| B | DHFR | C-terminus | .........VYEKKDGSRSHHHHHH |
| C | Thioredoxin | N-terminus | MRGSHHHHHHGSSDKIIHLT.......... |
| D | TNF-α | N-terminus | MRHHHHHHQSTESMIRDV......... |
| E | TFIIAγ | N-terminus | MGSSHHHHHHSSGLVPRGSH..... |
| F | Chaperonin | C-terminus | ..........AKIAKDGSRSHHHHHH |
| G | DNA polymerase | C-terminus | ..........EDWLSAKERSHHHHHH |
| H | TFIIAαß | Internal | ......QQQHQPQQQQHHHHHHHQQAQPQQTVP.... |
The QIAexpress System provides materials for the expression, purification, detection, and assay of 6xHis-tagged proteins.For detection,QIAexpress mouse monoclonal antibodies are produced under conditions that guarantee the highest purity and activity. Hybridoma cell culture is carried out under serum-free conditions, ensuring that the preparations are free of viruses, mycoplasma, and contaminating immunoglobulins. Purification by adsorption chromatography is performed entirely at physiological pH, yielding preparations with the highest proportion of native antibody.
Tetra·His Antibody allows efficient detection of His-tagged proteins, andis ideal for many applications, including colony, dot, and western blotting procedures (see figure"Sensitivity of Anti·His Antibodies"), screening for positive expression clones, andmonitoring expression levels and stability of 6xHis-tagged proteins. Tetra·His Antibodycombined withconjugates has many additional useful applications, including immunoprecipitation, ELISA, immunocytochemistry, immunohistochemistry, protein localization and targeting studies, and LabMAP bead-based assays.
Tetra·His Antibody allows efficient detection of His-tagged proteins and is ideal for many applications, including:
- Colony-, dot-, and western-blotting procedures
- Screening for positive expression clones
- Monitoring expression levels and stability of His-tagged proteins
- Immunoprecipitation and ELISA
- Immunocytochemistry and immunohistochemistry
Specifications
Features | Specifications |
| Detection | Secondary antibody required |
| Epitope detected | HHHH |
| Sensitivity in dot blots -chemiluminescent substrate- | 10 pg |
| Sensitivity in dot blots -chromogenic substrate- | 0.5 ng |
| Sensitivity in western blots - Chemiluminescent substrate | 50 pg |
| Sensitivity in western blots -chromogenic substrate- | 2 ng |
| Substrate for blot detection | Dependent on secondary antibody |
| Substrates for assay procedure | Dependent on secondary antibody |
| Tag | His tag |
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