Strep-tag Antibody
- Excellent results in dot- and western-blotting procedures
- Highly sensitive and specific detection
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- Specifications
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Cat No./ID:34850 Strep-tag Antibody (100 μg) $701.00 Mouse monoclonal antibody that recognizes the Strep-tag II epitope; lyophilized, for 1000 ml working solution |
Product Details
The Two-Step Affinity Purification System, which is based on the proven 6xHis tag and the short Strep-tag II, enables simple and highly efficient purification of ultrapure proteins in a fast and standardized procedure. Recombinant proteins carrying both tags are purified sequentially on Ni-NTA and Strep-Tactin matrices (see figure Ultrapure protein in two steps). The two simple affinity purifications provide fully active, full-length, ultrapure protein, suitable for any downstream application.
Recombinant proteins that carry two small affinity tags (the 6xHis tag and Strep-tag II) are efficiently expressed in E. coli, insect, or mammalian cells using pQE-TriSystem His·Strep vectors. After cell lysis and clearing of the lysate, proteins are initially purified using an immobilized-metal affinity chromatography procedure that is based on the proven 6xHis-tag-Ni-NTA interaction. After elution from the Ni-NTA matrix using imidazole, recombinant proteins (which also carry the Strep-tag II epitope) are loaded directly onto a Strep-Tactin matrix. No buffer exchange is required. Protein is eluted from the Strep-Tactin matrix using either biotin or desthiobiotin. This two-step affinity purification delivers ultrapure (>98% pure) protein (see figure Two-step affinity purification procedure). The order of purifications can be reversed (i.e., Strep-Tactin followed by Ni- NTA purification). Proteins can be detected with high specificity and sensitivity using mouse monoclonal Strep-tag or Anti·His antibodies.
The two-step affinity purification system, using the Strep-tag Antibody for detection, is highly suited for applications where high purity is at a premium or is difficult to achieve. The standardized purification procedure also increases throughput by eliminating the need for protein-specific purification protocol development and optimization. The ultrahigh purity and convenience provided by the two-step affinity purification system make it the method of choice for:
- Structural and functional analyses
- Expression in eukaryotic systems
Specifications
Features | Specifications |
| Applications | Western blot, dot blot, ELISA, immunoprecipitation, immunohistochemistry |
| Detection | Secondary antibody required |
| Epitope detected | SAWSHPQFEK |
| Sensitivity in Western blots (chemiluminescent detection) | 1 ng |
| Substrate for blot detection | Dependent on secondary antibody |
| Substrates for assay procedure | Dependent on secondary antibody |
| Tag | Strep-tag |
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