
Penta·His Biotin Conjugate
For sensitive detection of His-tagged proteins
- Highly specific and sensitive detection of His-tagged proteins
- Flexible detection using any steptavidin or avidin conjugate
In detection procedures, the Penta·His Conjugate binds to a protein"s His tag and the complex is localized using avidin derivatives, such as streptavidin, which can be conjugated to fluorescent dyes (e.g., streptavidin-R-PE) or to other proteins (e.g., HRP, AP) used in detection procedures.
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Cat No./ID:34440 Penta·His Biotin Conjugate $407.00 125 μl Penta·His Biotin Conjugate, 200 μg/ml |
Product Details
Property | Value |
---|---|
Detection: | Five consecutive histidine residues |
Antibody isotype: | Mouse IgG1 |
Cross-reactivity: | Negligible with crude E. coli, yeast, mammalian, or insect cell lysates |
Form: | Stabilized stock solution |
Working solution: | Dependent on application |
The Penta·His Biotin Conjugate can be used in conjunction with any streptavidin or avidin conjugate, delivering high flexibility in assay development.
Penta·His Biotin Conjugates allow colorimetric, chromogenic, chemiluminescent, or fluorescent detection of His-tagged proteins in western and dot blot, ELISA and other immunoassay procedures, and fluorescence-activated cell sorting (FACS).
Specifications
Features | Specifications |
Conjugated enzyme | Biotin |
Detection | Secondary antibody required |
Epitope detected | HHHHH |
Tag | His tag |
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