Qiagen/NTA Agarose/25 ml uncharged resin (max. pressure 2.8 psi)/30310
市场价：
¥6580.00
美元价：
3948.00
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
NTA Agarose
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For efficient immobilized-metal affinity chromatography (IMAC) using gravity-flow chromatography
- High binding affinity and high capacity
- Choice of purification under native or denaturing conditions
- Fine-tuning of purification strategies through flexible choice of metal ion
Uncharged NTA Agarose allows researchers to choose the metal ion they use for IMAC procedures, allowing fine-tuning of purification strategies. Metal ions such as Ni 2+, Cu 2+, Zn2+, or Co2+ are efficiently immobilized for IMAC purification of metal-binding proteins. NTA has four chelating sites to bind metal ions more tightly than other metal-chelating purification systems. This reduces metal-ion leaching and nonspecific binding and leads to increased protein binding capacity and purer preparations. A proprietary spacer that links NTA to the sepharose support provides optimal accessibility to metal ions, increasing protein-binding capacity.
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Cat No./ID:30310 NTA Agarose (25 ml) $329.00 25 ml uncharged resin (max. pressure 2.8 psi) |
The NTA Agarose is intended for molecular biology applications. This product is not intended for the diagnosis, prevention, or treatment of a disease.
Product Details
Performance
NTA Agarose enables efficient immobilized-metal affinity chromatography (IMAC) using gravity-flow chromatography.NTA has four chelation sites for nickel ions, which results in tighter binding of nickelcompared withmetal-chelating purification systems that only have three sites available for interaction with metal ions. The extra chelation site prevents nickel-ion leaching, assuring high binding affinity, resulting in a greater binding capacity and protein preparations withgreater purity (see figure "One-step purification under native conditions") than those obtained using other metal-chelating purification systems.
Principle
The QIAexpress Ni-NTA Protein Purification System is based on the remarkable selectivity of patented Ni-NTA (nickel-nitrilotriacetic acid) resin for proteins containing an affinity tag of six consecutive histidine residues — the 6xHis tag. This technology allows one-step purification of almost any His-tagged protein from any expression system under native or denaturing conditions. NTA, which has four chelation sites for nickel ions, binds nickel more tightly than metal-chelating purification systems that only have three sites available for interaction with metal ions. The extra chelation site prevents nickel-ion leaching and results in a greater binding capacity and protein preparations with higher purity (see figure "One-step purification under native conditions") than those obtained using other metal-chelating purification systems. The QIAexpress system can be used to purify His-tagged proteins from any expression system including baculovirus, mammalian cells, yeast, and bacteria.
Procedure
The purification of 6xHis-tagged proteins consists of 4 steps: cell lysis, binding, washing, and elution (see "Protein purification with the Ni-NTA protein purification system"). Purification of recombinant proteins using the QIAexpress system does not depend on the 3-dimensional structure of the protein or 6xHis tag. This allows one-step protein purification under either native or denaturing conditions, from dilute solutions and crude lysates. Strong denaturants and detergents can be used for efficient solubilization and purification of receptors, membrane proteins, and proteins that form inclusion bodies. Reagents that allow efficient removal of nonspecifically binding contaminants can be included in wash buffers (see table "Reagents compatible with the 6xHis/Ni-NTA interaction"). Purified proteins are eluted under mild conditions by adding 100–250 mM imidazole as competitor or by a reduction in pH.
| Denaturants | Detergents | Reducing agents | Others | Salts | For long-term storage |
|---|---|---|---|---|---|
| 6 M Gu·HCl | 2% Triton X-100 | 20 mM β-ME | 50% glycerol | 4 M MgCl2 | Up to 30% ethanol or 100 mM NaOH |
| 8 M Urea | 2% Tween 20 | 10 mM DTT | 20% ethanol | 5 mM CaCl2 | |
| 1% CHAPS | 20 mM imidazole | 2 M NaCl |
Applications
The QIAexpress Ni-NTA Protein Purification System provides reliable, one-step purification of proteins suitable for any application, including:
- Structural and functional investigations
- Crystallization for determination of three-dimensional structure
- Assays involving protein–protein and protein–DNA interactions (see QIAexpress Assay System)
- Immunization to produce antibodies
- Study molecular interactions with nucleic acids and binding proteins
- Purify antibodies
- Isolate nontagged, interacting subunits or nucleic acids
- Investigate ligand–receptor interactions
Specifications
Features | Specifications |
| Applications | Proteomics |
| Bead size | 45–165 µm |
| Binding capacity | Up to 50 mg/ml (up to 2.5 µmol @ ~20 kDa* * Based on immobilized nickel ion chromatography; capacities may vary for other ions. |
| Form | 50% suspension in 30% ethanol |
| FPLC | Yes |
| Gravity flow or spin column | Gravity flow |
| Processing | Manual/Automated |
| Scale | Large scale |
| Special feature | Batch and column purification |
| Start material | Cell lysate |
| Support/matrix | Sepharose CL-6B |
| Yield | 100 µg – 100 mg |
Product Resources
品牌介绍
QIAGEN是一家专业化致力于生物分子样品制备解决方案的跨国经营企业，总部位于德国。1984年，QIAGEN在德国成立，1996年在美国纽约纳斯达克上市。QIAGEN拥有超过1000项专利和认可证明，在18个国家设立了分公司，代理商服务国超过40个，在全球有超过400000的用户。QIAGEN提供的产品超过500类，包括各种试剂，耗材和自动化纯化工作站。这些产品用于样品采集，稳定，核酸或蛋白的分离，纯化和检测中，不仅广泛的应用于科研领域的各个方面，在生物技术，制药，法医研究，食品安全检测，畜牧业和分子诊断领域也得到了广泛的应用。QIAGEN产品的卓越品质和在应用中的出色表现使得其成为样品处理中标准的代名词。
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