Ni-NTA Superflow Columns
For gravity-flow purification of His-tagged proteins
- Up to 75 mg highly pure His-tagged protein per column
- Ready-to-use columns for gravity-flow purification
- Purification under native or denaturing conditions
Ni-NTA Superflow is available in convenient, pre-packed columns for purification of His-tagged proteins from manually prepared cleared lysates, which are applied to Ni-NTA Superflow Columns on a BioRobot vacuum manifold. His-tagged proteins are strongly and selectively bound to Ni-NTA.
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Cat No./ID:30622 Ni-NTA Superflow Columns (12 x 1.5 ml) $428.00 For 12 6xHis-tagged protein preps: 12 polypropylene columns containing 1.5 ml Ni-NTA Superflow |
Product Details
The QIAexpress Ni-NTA Protein Purification System, including the Ni-NTA Superflow Columns, is based on the remarkable selectivity of patented Ni-NTA (nickel-nitrilotriacetic acid) resin for proteins containing an affinity tag of six or more histidine residues — the His tag. This technology allows one-step purification of almost any His-tagged protein from any expression system under native or denaturing conditions. NTA, which has four chelation sites for nickel ions, binds nickel more tightly than metal-chelating purification systems that only have three sites available for interaction with metal ions. The extra chelation site prevents nickel-ion leaching and results in a greater binding capacity and protein preparations with higher purity than those obtained using other metal-chelating purification systems. The QIAexpress system can be used to purify His-tagged proteins from any expression system including baculovirus, mammalian cells, yeast, and bacteria. See figure Ni NTA Superflow 96 Columns automated procedure.
| Denaturants | Detergents | Reducing agents | Others | Salts | For long-term storage |
|---|---|---|---|---|---|
| 6 M Gu·HCl | 2% Triton X-100 | 20 mM β-ME | 50% glycerol | 4 M MgCl2 | Up to 30% ethanol |
| 8 M urea | 2% Tween 20 | 10 mM DTT | 20% ethanol | 5 mM CaCl2 | or 100 mM NaOH |
| 1% CHAPS | 20 mM TCEP | 20 mM imidazole* | 2 M NaCl |
The QIAexpress Ni-NTA Protein Purification System provides reliable, one-step purification of proteins suitable for any application, including:
- Structural and functional investigations
- Crystallization for determination of three-dimensional structure
- Assays involving protein–protein and protein–DNA interactions
- Immunization to produce antibodies
Specifications
Features | Specifications |
| Applications | Proteomics |
| Bead size | 60–160 µm |
| Binding capacity | 5–20 mg/ml |
| Gravity flow or spin column | Gravity flow or automated |
| Number of preps per run | 1–24 samples per run |
| Processing | Automated/manual |
| Scale | Large scale |
| Special feature | Cross-contamination-free |
| Start material | Cell lysate |
| Support/matrix | Superflow |
| Tag | 6xHis tag |
| Yield | <30> |
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