Qiagen/RGS·His Antibody, BSA-free/100 μg mouse anti·RGS(H)4 (lyophilized, BSA-free, for 1000 ml working solution)/34650
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¥14840.00
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8904.00
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4000-520-616
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商品介绍
RGS·His Antibody, BSA-free
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For sensitive detection of His-tagged proteins with an RGS·His epitope
- Highly specific detection of C-terminal, N-terminal, and internal His tags
- Highly sensitive and specific detection of RGS·His epitopes
Monoclonal mouse Anti·His Antibodies, like the RGS·His Antibody, BSA-free, are used to detect recombinant protein carrying His tags. RGS·His Antibody is one of three variants, each of which detects a different His tag epitope. Like all QIAGEN mouse monoclonal antibodies, RGS·His Antibody, BSA-free, is prepared under serum-free conditions, which guarantees the absence of viruses, mycoplasma, and contaminating immunoglobulins.
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Cat No./ID:34650 RGS·His Antibody, BSA-free (100 μg) $742.00 100 μg mouse anti·RGS(H)4 (lyophilized, BSA-free, for 1000ml working solution) |
The RGS·His Antibody, BSA-freeis intended for molecular biology applications. This product is not intended for the diagnosis, prevention, or treatment of a disease.
Product Details
Performance
| Property | Value |
|---|---|
| Detection: | N-terminal His tags (containing the epitope RGSHHHH) |
| Sensitivity in dot blots:* | 0.5 ng |
| Sensitivity in western blots:* | 2 ng |
| Isotype: | Mouse IgG1 |
| Cross-reactivity: | Negligible with crude E. coli, yeast, mammalian, or insect cell lysates |
| Form: | Lyophilized |
| Working solution: | 1000 ml |
Many different proteins have been detected, via their His tags, using the QIAexpress Anti·His Antibodies (see table). For the sensitivity of the three Anti·His Antibodies in detecting this panel of proteins see the figureSensitivity of QIAexpress Anti·His Antibodies)
| Protein | Tag location | Sequence |
|---|---|---|
| A DHFR | N-terminus | MRGSHHHHHHGSVRPLNCIV... |
| B DHFR | C-terminus | ...VYEKKDGSRSHHHHHH |
| C Thioredoxin | N-terminus | MRGSHHHHHHGSSDKIIHLT... |
| D TNFα | N-terminus | MRHHHHHHQSTESMIRDV... |
| E TFIIAγ | N-terminus | MGSSHHHHHHSSGLVPRGSH... |
| F Chaperonin | C-terminus | ...AKIAKDGSRSHHHHHH |
| G DNA Polymerase | C-terminus | ...EDWLSAKERSHHHHHH |
| H TFIIAαβ | Internal | ...QQQHQPQQQQHHHHHHQQAQPQQTVP... |
Principle
All QIAexpress mouse monoclonal antibodies are produced under conditions that guarantee the highest purity and activity. Hybridoma cell culture is carried out under serum-free conditions, ensuring that the preparations are free of viruses, mycoplasma, and contaminating immunoglobulins. Purification by adsorption chromatography is performed entirely at physiological pH, yielding preparations with the highest proportion of native antibody.
Procedure
The QIAexpress, Detection and Assay Handbook, supplied with all QIAexpress detection and assay products, contains detailed example protocols and guidelines for designing and performing assays.
Applications
QIAexpress Detection Systems, including RGS·His Antibody, BSA-free, allow efficient detection of His-tagged proteins and are ideal for many applications, including:
- Colony, dot, and western blotting procedures
- Screening for positive expression clones
- Monitoring expression levels and stability of His-tagged proteins
- Immunoprecipitation and ELISA
- Immunocytochemistry and immunohistochemistry
Specifications
Features | Specifications |
| Detection | Secondary antibody required |
| Epitope detected | RGSHHHH |
| Sensitivity in dot blots -chemiluminescent substrate- | 10 pg |
| Sensitivity in dot blots -chromogenic substrate- | 0.5 ng |
| Sensitivity in western blots - Chemiluminescent substrate | 50 pg |
| Sensitivity in western blots -chromogenic substrate- | 2 ng |
| Substrate for blot detection | Dependent on secondary antibody |
| Substrates for assay procedure | Dependent on secondary antibody |
| Tag | His tag |
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