Qiagen/QIAexpress Type IV Kit/5 μg each: pQE-30, pQE-31, pQE-32 (N-terminal 6xHis); 10 ml Ni-NTA Agarose/32149
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¥14820.00
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8892.00
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商品介绍
QIAexpress Type IV Kit
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For high-level expression and one-step purification of N-terminally His-tagged proteins
- Vectors covering all three reading frames
- Complete kit for expression and purification of His-tagged proteins
- High-level expression of N-terminally His-tagged proteins
- Versatile, complete system for one-step purification and sensitive detection
The QIAexpress System provides materials for expression, purification, detection, and assay of His-tagged proteins.
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- Product Details
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Cat No./ID:32149 QIAexpress Type IV Kit $741.00 5 μg each: pQE-30, pQE-31, pQE-32 (N-terminal 6xHis); 10 ml Ni-NTA Agarose |
The QIAexpress Type IV Kit is intended for molecular biology applications. This product is not intended for the diagnosis, prevention, or treatment of a disease.
Product Details
Performance
The QIAexpress Type IV Kit gives high expression, up to 50% of total cellular protein.
Principle
QIAexpress pQE vectors combine a powerful phage T5 promoter (recognized by E. coli RNA polymerase) with a double lac operator repression module to provide tightly regulated, high-level expression of recombinant proteins in E. coli. Protein synthesis is effectively blocked in the presence of high levels of lac repressor and the stability of cytotoxic constructs is enhanced. The pQE vectors (see figure pQE Vectors and table) enable placement of the His tag at either the N- or C-terminus of the recombinant protein.
| Element | Description |
|---|---|
| 1. Optimized promoter/operator element | Consists of the phage T5 promoter and two lac operator sequences, which increase the probability of lac repressor binding and ensure efficient repression of the powerful T5 promoter |
| 2. Synthetic ribosomal binding site RBSII | For efficient translation |
| 3. His-tag coding sequence | Either 5" or 3" to the polylinker cloning region |
| 4. Translational stop codons | In all reading frames for convenient preparation of expression constructs |
| 5. Two strong transcriptional terminators | t0 from phage lambda, and T1 from the rrnB operon of E. coli, to prevent read-through transcription and ensure stability of the expression construct |
| 6. ColE1 origin of replication | From pBR322 |
| 7. beta-lactamase gene (bla) | Confers ampicillin resistance |
Procedure
Inserts encoding proteins of interest are cloned into appropriate constructs and transformed into a suitable E. coli strain for expression. Expression is induced by addition of IPTG. QIAexpress Type IV Kit constructs can be transformed into E. coli, used as a shuttle vector for recombinant protein expression in insect cells, or transfected into mammalian cells.
Applications
The QIAexpress Expression System provides high-level expression of proteins suitable for many applications, including:
- Purification of functional, conformationally active proteins
- Purification under denaturing conditions for antibody production
- Crystallization for determination of three-dimensional structure
- Assays involving protein-protein and protein-DNA interactions
Specifications
Features | Specifications |
| Applications | Proteomics |
| N- or C-terminal tag | N-terminal tag |
| Processing | Manual |
| Special feature | Versatile, complete system for one-step purification |
| Start material | Cell lysate |
| Tag | 6xHis tag |
| Yield | <50% of="" total=""> |
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品牌介绍
QIAGEN是一家专业化致力于生物分子样品制备解决方案的跨国经营企业，总部位于德国。1984年，QIAGEN在德国成立，1996年在美国纽约纳斯达克上市。QIAGEN拥有超过1000项专利和认可证明，在18个国家设立了分公司，代理商服务国超过40个，在全球有超过400000的用户。QIAGEN提供的产品超过500类，包括各种试剂，耗材和自动化纯化工作站。这些产品用于样品采集，稳定，核酸或蛋白的分离，纯化和检测中，不仅广泛的应用于科研领域的各个方面，在生物技术，制药，法医研究，食品安全检测，畜牧业和分子诊断领域也得到了广泛的应用。QIAGEN产品的卓越品质和在应用中的出色表现使得其成为样品处理中标准的代名词。
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