RGS·His HRP Conjugate Kit
For sensitive, direct detection of His-tagged proteins with RGS·His epitopes
- High-sensitivity and high-speed detection of His-tagged proteins
- No need for secondary antibody
- Optimized blocking reagent supplied
Anti·His HRP Conjugates, including the RGS·His HRP Conjugate, are intended for sensitive detection of recombinant proteins carrying His tags, without the need for secondary antibodies. They consist of Anti·His antibodies conjugated to horseradish peroxidase (HRP). In detection procedures, the Anti·His antibody binds to a protein"s His tag and the complex is localized using chemiluminescent or chromogenic substrates that react with HRP.
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Cat No./ID:34450 RGS·His HRP Conjugate Kit $483.00 125 μl RGS·His HRP Conjugate, 5g Blocking Reagent, 50ml Blocking Reagent Buffer (10xconcentrate) |
Product Details
| Property | Value |
|---|---|
| Detection: | N-terminal, C-terminal, and internal His tags (containing the epitope RGSHHHH) |
| Sensitivity in dot blots:* | 10 pg |
| Sensitivity in western blots:* | 50 pg |
| Antibody Isotype: | Mouse IgG1 |
| Cross-reactivity: | Negligible with crude E. coli, yeast, mammalian, or insect cell lysates |
| Form: | Stabilized stock solution |
| Working solution: | 125–250 ml |
Anti·His HRP Conjugates consist of the QIAexpress Anti·His Antibodies chemically coupled to the reporter enzyme horseradish peroxidase (HRP). They can be used for highly specific and sensitive, direct detection of any recombinant protein that has an accessible His tag. They eliminate the need for secondary antibodies, saving time in immunodetection procedures. Anti·His HRP Conjugates can be used with chemiluminescent or chromogenic detection substrates.
Anti·His HRP Conjugates, including the RGS·His HRP Conjugate, recognize the same epitopes as their corresponding antibody and can be used for direct chemiluminescent or chromogenic detection of His-tagged proteins in:
- Western-, dot-, and colony-blotting procedures
- ELISA and other immunoassay procedures
Specifications
Features | Specifications |
| Conjugated enzyme | HRP |
| Detection | Secondary antibody required |
| Epitope detected | RGSHHHH |
| Substrate for blot detection | Biotech ECL |
| Substrates for assay procedure | OPD |
| Tag | His tag |
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