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商品详细Qiagen/Qproteome Mitochondria Isolation Kit/Buffers and reagents for 12 high-purity mitochondrial preparations/37612
Qiagen/Qproteome Mitochondria Isolation Kit/Buffers and reagents for 12 high-purity mitochondrial preparations/37612
Qiagen/Qproteome Mitochondria Isolation Kit/Buffers and reagents for 12 high-purity mitochondrial preparations/37612
商品编号: 37612
品牌: Qiagen
市场价: ¥7660.00
美元价: 4596.00
产地: 美国(厂家直采)
公司:
产品分类: 多肽合成
公司分类: peptide
联系Q Q: 3392242852
电话号码: 4000-520-616
电子邮箱: info@ebiomall.com
商品介绍

Qproteome Mitochondria Isolation Kit

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For efficient isolation of high-purity mitochondria preparations
  • High-purity, intact mitochondria
  • Highly reproducible using a standardized procedure
  • Convenient, fast, and easy-to-use kit format

The Qproteome Mitochondria Isolation Kit provides fully active mitochondrial preparations of very high purity in a fast, reproducible, and standardized procedure that uses a standard bench-top centrifuge.

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Cat No./ID:37612
Qproteome Mitochondria Isolation Kit
$383.00
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Buffers and reagents for 12 high-purity mitochondrial preparations
The Qproteome Mitochondria Isolation Kit is intended for molecular biology applications. This product is not intended for the diagnosis, prevention, or treatment of a disease.

Product Details

9
Mitochondrial fractions virtually free of cross-contamination.
Western blots of mitochondrial preparations probed with antibodies specific for organelle-/cell-compartment specific marker proteins. PM: plasma membrane; ER: endoplasmic reticulum.
9
High purity mitochondria with intact mitochondrial membranes.
[A] Mitochondria were prepared from 60 mg of liver tissue using the Qproteome Mitochondria Isolation Kit and subjected to 2D-PAGE. Out of 384 spots picked, 259 proteins were identified by MALDI-MS, 219 (85%) of which were predicted to have a mitochondrial localization. Green circles indicate mitochondrial proteins, red circles indicate impurities, the black circle indicates a protein which is probably located in mitochondria and ER. [B] Electron micrographs of mitochondria isolated using the Qproteome Mitochondria Isolation Kit showing intact inner and outer membranes.
9
More intact mitochondria per milligram protein.

Mitochondria were isolated using the Qproteome Mitochondria Isolation Kit or another commercially available kit (Supplier S) according to manufacturer’s instructions. Mitochondrial membrane integrity is indicated by formation of fluorescent aggregates following uptake of a carbocyanine dye.

Performance
In contrast to a differential centrifugation method from the literature, analysis of cell-compartment marker proteins shows that the Qproteome Mitochondria Isolation Kit provides mitochondrial fractions virtually free of cross-contamination (see figure "Mitochondrial fractions virtually free of cross-contamination"). The virtual absence of cytosolic contaminants greatly facilitates analysis of low-abundance species as shown in 2D-PAGE analyses and the gentle procedure provides a highly enriched fraction containing intact mitochondria (see figure "High purity mitochondria with intact mitochondrial membranes"). Integrity can also be demonstrated by measuring uptake of a fluorescent dye (see figure "More intact mitochondria per milligram protein").
Principle

One of the major problems facing researchers working with mitochondria is efficiently separating mitochondrial and cytosolic fractions. Traditional preparation methods are tedious, time-consuming, and require access to an ultracentrifuge. The Qproteome Mitochondria Isolation Kit provides fully active mitochondrial preparations of very high purity in a fast, reproducible, and standardized procedure that uses a standard bench-top centrifuge.

Applications

Mitochondria are the subject of intense study in a number of fields, including apoptosis, proteomics, biomedical research, and drug discovery. The Qproteome Mitochondria Isolation Kit delivers high-purity mitochondria whose proteins retain their native conformation and full biological activity — making them highly suited for apoptosis studies, 2D-PAGE analysis, and other biochemical assays.

Specifications

Features
Specifications
ApplicationsSDS-PAGE, mass spectrometry, activity assays
Binding capacity/yield20–80 µg
For glycoproteins: which type of glycoproteinsn.d
Fractions isolatedOne fraction
Sample size5 x 10e6 - 2 x 10e7 cells, 20 mg - 60 mg
SpeciesEukaryotes
Start materialCells, Tissue

Product Resources

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FAQs (8)
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What starting material can be used with the Qproteome Mitochondria Isolation Kit?
FAQ ID -1184
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Can the nuclei and cell debris fraction generated in the QProteome Mitochondria isolation procedure be used for further fractionation?
FAQ ID -1185
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Do I need an ultracentrifuge for the density gradient centrifugation step in the Qproteome Mitochondria Isolation protocol?
FAQ ID -1186
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Is it possible to collect intact mitochondria before or after the density gradient centrifugation step of the Qproteome Mitochondria Isolation protocol?
FAQ ID -1187
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Can mitochondria separated with the Qproteome Mitochondria Isolation Kit be used for downstream DNA isolation?
FAQ ID -1188
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How many cells can be used with the Qproteome Mitochondria Isolation Kit, and how much mitochondrial protein can be expected?
FAQ ID -1189
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Do you have a kit for protein isolation from mitochondria prepared with your Qproteome Mitochondria Isolation Kit?
FAQ ID -1190
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Which Qproteome or Protein Fractionation Kits from QIAGEN are compatible with tissue samples?
FAQ ID -755
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Kit Handbooks (1)
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(EN) - Qproteome Mitochondria Isolation Handbook
For purification of mitochondria from eukaryotic cells and tissues
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Technical Information (1)
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(EN) - Standardized and reproducible protein fractionation and depletion with Qproteome Kits
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Scientific Posters (2)
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(EN) - A range of ready-to-use kits reduces complexity of protein samples for proteomics studies
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(EN) - Efficient processing of tissue samples using different protein fractionation technologies
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Quick-Start Protocols (1)
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Qproteome Mitochondria Isolation Kit Quick-Start Protocol (EN)
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Safety Data Sheets (1)
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MSDS Qproteome Mitochondria Isolation Kit
fragment fix placeholder

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品牌介绍
QIAGEN是一家专业化致力于生物分子样品制备解决方案的跨国经营企业,总部位于德国。1984年,QIAGEN在德国成立,1996年在美国纽约纳斯达克上市。QIAGEN拥有超过1000项专利和认可证明,在18个国家设立了分公司,代理商服务国超过40个,在全球有超过400000的用户。QIAGEN提供的产品超过500类,包括各种试剂,耗材和自动化纯化工作站。这些产品用于样品采集,稳定,核酸或蛋白的分离,纯化和检测中,不仅广泛的应用于科研领域的各个方面,在生物技术,制药,法医研究,食品安全检测,畜牧业和分子诊断领域也得到了广泛的应用。QIAGEN产品的卓越品质和在应用中的出色表现使得其成为样品处理中标准的代名词。