Qiagen/Qproteome Cell Compartment Kit/For up to 10 subcellular fractionations*: Extraction buffers, Protease Inhibitor Solution, Benzonase®./37502
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3864.00
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商品介绍
Qproteome Cell Compartment Kit
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For fractionation of proteins according to cellular location
 
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Cat No./ID:37502 Qproteome Cell Compartment Kit $322.00 For up to 10 subcellular fractionations*: Extraction buffers, Protease Inhibitor Solution, Benzonase®. |
The Qproteome Cell Compartment Kit is intended for molecular biology applications. This product is not intended for the diagnosis, prevention, or treatment of a disease.
Product Details
Performance
Use of the Qproteome Cell Compartment Kit allows simple and effective cell compartment-specific localization of marker proteins from isolated cells (see figure "Specific Separation of Marker Proteins" and to "Localization of Proteins in Cells Under Different Growth Conditions") or from tissue samples (see figure "Localization of Proteins in Tissue Samples").
Principle
The kit contains 4 Extraction Buffers which enable the sequential isolation of proteins associated with the cytosol, membranes, nucleus, and cytoskeleton from cell lysates or tissues.
Procedure
The 4 Extraction Buffers are added sequentially to a cell pellet and the respective fractions are isolated by centrifugation (see flowchart).
In an alternative protocol for tissues, samples are simultaneously homogenized and disrupted using the TissueRuptor before being processed as for the cell pellet.
Applications
Subcellular fractionation of proteins can be used for the enrichment of low-abundance species; to define the subcellular localization of enzymes, regulatory, and structural proteins; and for monitoring of compartmental redistribution of biomolecules under basal and stimulated conditions.
Specifications
Features | Specifications |
| Applications | SDS-PAGE, mass spectrometry |
| Binding capacity/yield | Varies |
| Fractions isolated | Four fractions |
| Sample size | ~5 x 10e6 cells |
| Species | Eukaryotes |
| Start material | Cell lysate |
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品牌介绍
QIAGEN是一家专业化致力于生物分子样品制备解决方案的跨国经营企业，总部位于德国。1984年，QIAGEN在德国成立，1996年在美国纽约纳斯达克上市。QIAGEN拥有超过1000项专利和认可证明，在18个国家设立了分公司，代理商服务国超过40个，在全球有超过400000的用户。QIAGEN提供的产品超过500类，包括各种试剂，耗材和自动化纯化工作站。这些产品用于样品采集，稳定，核酸或蛋白的分离，纯化和检测中，不仅广泛的应用于科研领域的各个方面，在生物技术，制药，法医研究，食品安全检测，畜牧业和分子诊断领域也得到了广泛的应用。QIAGEN产品的卓越品质和在应用中的出色表现使得其成为样品处理中标准的代名词。
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