Qiagen/Qproteome核蛋白试剂盒/用于12种核蛋白制剂：缓冲液、试剂、蛋白酶抑制剂溶液、苯甲酸酶/37582
市场价：
¥9720.00
美元价：
5832.00
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
Qproteome Nuclear Protein Kit
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For separation of nuclear and nucleic acid binding proteins
- Nuclear preparations free of cytosolic proteins
- Greatly reduced sample complexity
- Highly reproducible fractionation
- Includes protocols for tissue proteomics
The Qproteome Nuclear Protein Kit is designed for specific enrichment of nuclear proteins from cultured mammalian cells.
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- Product Details
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Cat No./ID:37582 Qproteome Nuclear Protein Kit $486.00 For 12 nuclear protein preparations: Buffers, Reagents, Protease Inhibitor Solution, Benzonase |
The Qproteome Nuclear Protein Kit is intended for molecular biology applications. This product is not intended for the diagnosis, prevention, or treatment of a disease.
Product Details
Performance
The Qproteome Nuclear Protein Kit provides highly effective and reproducible separation of nuclear proteins as demonstrated using specific marker proteins (see figure "Reproducible, efficient separation of marker proteins"), which remain functionally active (see figure "Isolation of an active transcription factor").
Principle
The Qproteome Nuclear Protein Kit is designed for specific enrichment of nuclear proteins from cultured mammalian cells. Lysis and centrifugation are used to separate the cytosolic fraction (supernatant) from the cell nuclei (pellet). A high-salt buffer allows dissociation of nuclear binding proteins (such as transcription factors) and their removal by diffusion from the nuclei.
Procedure
Cells are lysed and centrifuged to isolate nuclei. After washing, an extraction buffer is added to the nuclei and nucleic acid binding proteins dissociate from DNA and RNA. This soluble fraction is separated from the nuclear pellet by centrifugation. Proteins remaining in the pellet are solubilized using a second extraction buffer (see figure "Nuclear protein fractionation procedure").
Applications
The Qproteome Nuclear Protein Kit delivers a nucleic acid binding protein fraction suitable for a wide range of activity assays.
Specifications
Features | Specifications |
| Applications | SDS-PAGE, mass spectrometry |
| Binding capacity/yield | 200–300 µg |
| For glycoproteins: which type of glycoproteins | n.d |
| Fractions isolated | Three fractions |
| Sample size | 5 x 10e6 cells |
| Species | Mammal |
| Start material | Cell cultures |
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品牌介绍
QIAGEN是一家专业化致力于生物分子样品制备解决方案的跨国经营企业，总部位于德国。1984年，QIAGEN在德国成立，1996年在美国纽约纳斯达克上市。QIAGEN拥有超过1000项专利和认可证明，在18个国家设立了分公司，代理商服务国超过40个，在全球有超过400000的用户。QIAGEN提供的产品超过500类，包括各种试剂，耗材和自动化纯化工作站。这些产品用于样品采集，稳定，核酸或蛋白的分离，纯化和检测中，不仅广泛的应用于科研领域的各个方面，在生物技术，制药，法医研究，食品安全检测，畜牧业和分子诊断领域也得到了广泛的应用。QIAGEN产品的卓越品质和在应用中的出色表现使得其成为样品处理中标准的代名词。
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