Qproteome Mammalian Protein Prep Kit
- Gentle but effective cell lysis for high yields of active proteins
- Simple procedure, no specialized equipment required
- Highly reproducible procedure, consistent results time after time
The kit contains a lysis buffer for total protein preparations from mammalian cells for use in proteomics procedures.
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Cat No./ID:37901 Qproteome Mammalian Protein Prep Kit $234.00 For approximately 100 protein preparations from cultured mammalian cells: Buffer, Reagents, Protease Inhibitor Solution, Benzonase |
Product Details
The Qproteome Mammalian Protein Prep Kit enables efficient extraction of total protein from cultured mammalian cells (see figure "Efficient extraction of proteins from cultured cells").
The efficiency of extraction is demonstrated in the table below:
| Cell line | Protein yield (µg) | Solubilization (%) |
|---|---|---|
| COS | 660 | 93 |
| HeLa | 1580 | 97 |
| CHO | 1120 | 98 |
| HIH | 660 | 97 |
| Jurkat | 530 | 96 |
The Qproteome Mammalian Protein Prep Kit provides gentle but efficient detergent-based lysis of mammalian cells to deliver a soluble protein fraction suitable for any downstream application. Cell pellets are incubated with Mammalian Cell Lysis Buffer supplemented with Benzonase — an enzyme that digests nucleic acids — and a protease inhibitor, which protects the released proteins from enzymatic degradation. No specialized equipment is required.
Cells are simply incubated with lysis buffer and centrifuged to separate cell debris. The supernatant contains the soluble protein fraction, which can be further purified or directly analyzed, for example by SDS-PAGE.
The Qproteome Mammalian Protein Prep Kit is used for total protein preparations from mammalian cells for proteomics procedures (e.g., 2D-PAGE, mass spectrometry).
Specifications
Features | Specifications |
| Applications | SDS-PAGE, mass spectrometry |
| Binding capacity/yield | 780–1700 µg |
| Fractions isolated | One fraction |
| Sample size | 5–10 x 10e6 cells |
| Species | Mammal |
| Start material | Cell lysate |
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