Qiagen/Qproteome Bacterial Protein Prep Kit/For soluble protein preparations from up to 4.5 liters of bacterial cell culture: Lysis Buffer, Lysozyme, Benzonase®/37900
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¥4740.00
美元价：
2844.00
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3392242852
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4000-520-616
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商品介绍
Qproteome Bacterial Protein Prep Kit
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For preparation of soluble bacterial proteins
- Gentle but effective cell lysis, high yields of active proteins
- Simple procedure, no specialized equipment required
- Highly reproducible procedure, consistent results time after time
The kit contains a lysis buffer for preparation of soluble proteins from bacterial cultures for use in proteomics procedures.
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- Product Details
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Cat No./ID:37900 Qproteome Bacterial Protein Prep Kit $237.00 For soluble protein preparations from up to 4.5 liters of bacterial cell culture: Lysis Buffer, Lysozyme, Benzonase® |
The Qproteome Bacterial Protein Prep Kit is intended for molecular biology applications. This product is not intended for the diagnosis, prevention, or treatment of a disease.
Product Details
Principle
Bacterial cell walls are disrupted by the combination of a freeze–thaw cycle and a lysis buffer containing lysozyme. The lysis buffer also contains a nuclease, which reduces the viscosity of the lysate and frees proteins associated with nucleic acids. Once lysis is complete, the lysate is centrifuged to precipitate insoluble proteins and cell debris.
Procedure
Lysis buffer is added to frozen cell pellets, cells are resuspended, and incubated on ice for 30 minutes. The lysate is centrifuged to precipitate insoluble proteins and cell debris, and soluble proteins are collected in the supernatant.
Applications
The Qproteome Bacterial Protein Prep Kit is used for preparation of soluble bacterial proteins for proteomics procedures (e.g., 2D-PAGE, mass spectrometry).
Specifications
Features | Specifications |
| Applications | SDS-PAGE, mass spectrometry |
| Fractions isolated | One fraction |
| Sample size | Pellet from a 250 ml sample |
| Species | Bacteria |
| Start material | Cell cultures |
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品牌介绍
QIAGEN是一家专业化致力于生物分子样品制备解决方案的跨国经营企业，总部位于德国。1984年，QIAGEN在德国成立，1996年在美国纽约纳斯达克上市。QIAGEN拥有超过1000项专利和认可证明，在18个国家设立了分公司，代理商服务国超过40个，在全球有超过400000的用户。QIAGEN提供的产品超过500类，包括各种试剂，耗材和自动化纯化工作站。这些产品用于样品采集，稳定，核酸或蛋白的分离，纯化和检测中，不仅广泛的应用于科研领域的各个方面，在生物技术，制药，法医研究，食品安全检测，畜牧业和分子诊断领域也得到了广泛的应用。QIAGEN产品的卓越品质和在应用中的出色表现使得其成为样品处理中标准的代名词。
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