Qiagen/QIAamp 96 Virus QIAcube HT Kit/For 480 preps: QIAamp 96 plates, QIAGEN Proteinase K, carrier RNA, buffers/57731
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¥31060.00
美元价：
18636.00
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3392242852
电话号码：
4000-520-616
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info@ebiomall.com
商品介绍
QIAamp 96 Virus QIAcube HT Kit
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For automated high-throughput purification of viral RNA and DNA from a variety of samples
- Simple and reliable automated processing for cost and time savings
- Suitable for many samples, including blood, tissues, swabs and body fluids
- Consistent, high yields
- Efficient removal of inhibitors and contaminants
- Purified nucleic acids ready for analysis by real-time PCR or RT-PCR
The QIAamp 96 Virus QIAcube HT Kit enables simple, automated purification of viral RNA and DNA on the QIAcube HTsystem. Using proven QIAamp silica-membrane technology in a convenient 96-well format, contaminants and inhibitors are removed to yield high-quality nucleic acids that are ready for downstream analysis.
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Cat No./ID:57731 QIAamp 96 Virus QIAcube HT Kit (5) $1,553.00 For 480 preps: QIAamp 96 plates, QIAGEN Proteinase K, carrier RNA, buffers |
Cat No./ID:950067 QIAcube HT Plasticware $321.00 Plasticware for 480 typical preps on QIAcube HT: 5S-Blocks, 5 Elution Microtubes RS (EMTR), 120x8-Well Strip Caps for EMTR, 9 x 96 Filter-Tips OnCor C, TapePad |
The QIAamp 96 Virus QIAcube HT Kit is intended for molecular biology applications. This product is not intended for the diagnosis, prevention, or treatment of a disease.
Product Details
Performance
The QIAamp 96 Virus QIAcube HT Kit enables automated purification of viral RNA and DNA from a broad range of sample types including fresh or frozen tissues, blood and other body fluids.The procedure yields high-quality viral nucleic acids that perform well in downstream PCR and RT-PCR analyses (see figure Reliable purification from a range of sample types).QIAcube HT with the dedicated QIAamp 96 Virus QIAcube HT Kit lets users increase sample purification throughput without having to compromise on quality or reliability.The procedureprovides high yields of pure RNA or DNA that perform well in downstream analyses, similar to other QIAGEN DNA purification solutions (see figuresReliable automated purification and High sensitivity of viral nucleic acids in PCR and RT-PCR).
Principle
The QIAamp 96 Virus QIAcube HT Kit combines the selective binding properties of a silica-based membrane with a high-throughput 96-well format, and is designed for fully automated, simultaneous processing of 24–96 samples on the QIAcube HT instrument.
| Specification | Description |
|---|---|
| Number of samples | 24–96 samples (to be processed in increments of 8) |
| Sample input volume | Blood and other body fluids: up to 200 μl (for sample volumes less than 200 μl, add PBS) Tissues:up to 20mg tissue |
| Elutionvolume | 100 μl |
| Duration | 96 samples in approximately145 minutes 24 samples in approximately 90 minutes |
Procedure
The QIAamp 96 Virus QIAcube HT procedure is fast and simple with the QIAcube HT instrument. Samples are lysed under highly denaturing conditions at room temperature in the presence of QIAGEN proteinase K and Buffer ACL, which together ensure the inactivation of nucleases. Adding Buffer ACB adjusts the binding conditions for the co-purification of DNA and RNA. The lysate is then transferred to a QIAamp 96 plate. During vacuum, nucleic acids are adsorbed onto the silica membranes while contaminants pass through. Three efficient wash steps remove the remaining contaminants and enzyme inhibitors, and nucleic acids are eluted in Buffer AVE. Some samples may require a pretreatment.
Applications
The high-quality nucleic acids are ready to use in all downstream applications, including sensitive detection assays using quantitative, real-time PCR or RT-PCR.
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品牌介绍
QIAGEN是一家专业化致力于生物分子样品制备解决方案的跨国经营企业，总部位于德国。1984年，QIAGEN在德国成立，1996年在美国纽约纳斯达克上市。QIAGEN拥有超过1000项专利和认可证明，在18个国家设立了分公司，代理商服务国超过40个，在全球有超过400000的用户。QIAGEN提供的产品超过500类，包括各种试剂，耗材和自动化纯化工作站。这些产品用于样品采集，稳定，核酸或蛋白的分离，纯化和检测中，不仅广泛的应用于科研领域的各个方面，在生物技术，制药，法医研究，食品安全检测，畜牧业和分子诊断领域也得到了广泛的应用。QIAGEN产品的卓越品质和在应用中的出色表现使得其成为样品处理中标准的代名词。
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