QIAsymphony Certal Kits

QIAsymphony Certal Kits, in combination with the QIAsymphony SP, enable fully automated purification of nucleic acids from samples generated during production and purification of cell-culture-based biotechnology-derived products, biopharmaceutical protein drugs (e.g., monoclonal antibodies), or from virus-propagating cell lines for vaccine production. Samples include cell-culture supernatants or fermentation broths after removal of cells, bioprocess purification buffers from chromatography purification procedures, or fractions from vaccine purification (e.g., after inactivation).

The QIAsymphony Certal Residual DNA Kit enables extraction of residual host cell DNA and viral nucleic acids from biologics (e.g., monoclonal antibodies) purified using chromatography procedures. A proprietary buffer (buffer CA) is used to equilibrate matrix effects caused by chromatography reagents, thereby improving the recovery of the nucleic acids from bioprocess samples.

The QIAsymphony Certal Vaccine NA Kit enables extraction of viral nucleic acids from cell-based vaccine production. A proprietary buffer (buffer CB) is used to equilibrate for matrix effects caused by chemical or physical treatment during inactivation and purification of the propagated virus from vaccine production.

Proven, performance-leading magnetic particle technology enables purification of high-quality nucleic acids that are free of proteins, nucleases, and other impurities. The purified nucleic acids are ready for direct use in downstream applications, such as amplification or other detection reactions (e.g., Threshold assay). The QIAsymphony SP performs all steps of the purification procedure. Up to 96 samples, in batches of up to 24, are processed in a single run.

For downstream detection, the QIAsymphony Certal Kits are compatible with the Certal CHO Detection Kit and the Certal Vero Detection Kit, enabling highly sensitive detection of residual genomic DNA from CHO and Vero cell lines using quantitative PCR. For high-precision results, we recommend using the Rotor-Gene Q real-time PCR cycler.

QIAsymphony technology combines the speed and efficiency of silica-based nucleic acid purification with the convenient handling of magnetic particles. The purification procedure is designed to ensure safe and reproducible handling of potentially infectious samples, and comprises 4 steps: lyse, bind, wash, and elute. The user can choose between different eluation volumes (60 μl, 85 μl, and 110 μl), depending on the protocol (see table).

Worktable setup is rapid, which saves your valuable time. Simply place up to 2 reagent cartridges in the consumables drawer. The reagent cartridges can be either from the same kit or from different kits, enabling different purification procedures to be performed within the same run of 96 samples. Reagent volumes appropriate for the selected number of samples are used, giving you complete cost control.

No pre-conditioning with either buffer CA or buffer CB is necessary for the extraction of host-cell DNA or viral nucleic acids from cell-culture supernatant or fermentation broth.

QIAsymphony Certal Kits, in combination with the QIAsymphony SP, enable purification of residual host cell DNA and viral nucleic acids from bioprocess purification buffer, cell culture supernatant samples, and vaccine preparations.