Qiagen/QIAamp ccfDNA/RNA试剂盒/适用于50种制备方法：RNeasy Midi和RNeasy MinElute旋转柱、收集管（50 ml）、洗脱管（1.5 ml和2 ml）、无核糖核酸酶试剂和缓冲液/55184
市场价：
¥23340.00
美元价：
14004.00
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
QIAamp ccfDNA/RNA Kit
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For purification of cell-free DNA and RNA from plasma and serum samples
- Co-purification of DNA and RNA into one eluate
- Easy bench-top, vacuum-freeprotocol using spin column technology
- Small elution volumes
The QIAamp ccfDNA/RNA Kit enables co-purification of cell-free DNA and RNA, including vesicular and non-vesicular nucleic acids. Starting from 1–4 ml plasma, the protocol uses midi spin columns, removing the need for a vacuum step. For nucleic acid elution, MinElute spin columns allow for small elution volumes of 14 µl. The kit ensures high yields of cell-free DNA and total RNA including miRNA from plasma and serum samples.
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- Product Details
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Cat No./ID:55184 QIAamp ccfDNA/RNA Kit (50) $1,167.00 For 50 preps: RNeasy Midi and RNeasy MinElute Spin Columns, Collection Tubes (50 ml), Elution Tubes (1.5 ml and 2 ml), RNase-Free Reagents and Buffers |
The QIAamp ccfDNA/RNA Kit is intended for molecular biology applications. This product is not intended for the diagnosis, prevention, or treatment of a disease.
Product Details
Performance
The QIAamp ccfDNA/RNA protocol is specially designed to allow both DNA and RNA elution from 1–4 ml plasma or serum (see figure QIAamp ccfDNA/RNA Kit procedure). Yields of cell-free DNA and RNA are comparable to or better than the QIAamp Circulating Nucleic Acid Kit (see figure High recovery of ccfDNA and RNA compared to the QIAamp Circulating Nucleic Acid Kit). Unlike other kits, the QIAamp ccfDNA/RNA Kitensures high yields of RNA and DNA (see figure Competitive performance without compromising on DNA or RNA yields). The QIAamp ccfDNA/RNA Kit further achieves higher RNA integrity than other kits, making it broadly suited for any downstream application (see figure High integrity of RNA).
Principle
The QIAamp ccfDNA/RNA Kit provides an efficient way to isolate both DNA and RNA from the same plasma or serum sample. The procedure uses midi spin columns, allowing vacuum-free processing of 1–4 ml sample input volume (see figure Scalable yields of cfDNA and RNA between 1–4 ml plasma sample amount). Small elution volumes are achieved using MinElute columns. The complete protocol is phenol-free so that it can becarried outwithout working under a fume hood. The protocol and reagents have been optimized to ensure high yields of DNA and RNA.
Procedure
The QIAamp ccfDNA/RNA Kit protocol consists of initial cell-free DNA/RNA isolation, followed by cleanup and concentration. The cleanup steps can be automated on the QIAcube. Cell-free DNA/RNA isolationSerum or plasma (1–4 ml) are mixed with Buffer RPL to lyse exosomes and release RNA. The sample is then mixed with Buffer RPP and centrifuged to precipitate proteins. Isopropanol is added to the supernatant to provide the appropriate conditions for nucleic acids to bind to the silica membrane. The sample is then applied to the midi spin column, where the DNA and RNA molecules bind to the membrane. The column is washed twice with different buffers before the cell-free DNA and RNA is eluted simultaneously.CleanupLysis buffer and ethanol are added to the eluate before transfer to an RNeasy MinElute column. The column is subsequently washed with wash buffer and ethanol before the cell-free DNA and RNA is eluted.
Applications
For any liquid biopsy sample that requires parallel analysis of DNA and RNA mutations or for samples with low mutant allele frequencies, the QIAamp ccfDNA/RNA Kit efficiently isolates complete DNA and RNA, including miRNA,from any plasma or serum sample.
Product Resources
品牌介绍
QIAGEN是一家专业化致力于生物分子样品制备解决方案的跨国经营企业，总部位于德国。1984年，QIAGEN在德国成立，1996年在美国纽约纳斯达克上市。QIAGEN拥有超过1000项专利和认可证明，在18个国家设立了分公司，代理商服务国超过40个，在全球有超过400000的用户。QIAGEN提供的产品超过500类，包括各种试剂，耗材和自动化纯化工作站。这些产品用于样品采集，稳定，核酸或蛋白的分离，纯化和检测中，不仅广泛的应用于科研领域的各个方面，在生物技术，制药，法医研究，食品安全检测，畜牧业和分子诊断领域也得到了广泛的应用。QIAGEN产品的卓越品质和在应用中的出色表现使得其成为样品处理中标准的代名词。
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