Qiagen/AllPrep PowerFecal DNA/RNA试剂盒/适用于50个Prep：50个AllPrep DNA MinElute旋转柱、50个RNeasy微型旋转柱、收集管、洗脱管和缓冲液/80244
市场价：
¥11300.00
美元价：
6780.00
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
AllPrep PowerFecal DNA/RNA Kit
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For simultaneous purification of genomic DNA and total RNA from stool samples
- Efficient removal of inhibitors through Inhibitor Removal Technology (IRT)
- Separates RNA and DNA in different eluates for easier downstream analysis
- Optimized lysis of microbial cells for increased DNA and RNA yields
- Enables comprehensive and comparative metagenomic and metatranscriptomic analysis
For reliable comparison of genomic and transcriptomic data, purification of DNA and RNA from the same sample is essential. The AllPrep PowerFecal DNA/RNA Kit protocol enables the simultaneous isolation of DNA and RNA in separate eluates from one sample. The kit is optimized for use in metagenomic and metatranscriptomic analyses using next-generation sequencing (NGS).
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Cat No./ID:80244 AllPrep PowerFecal DNA/RNA KitDiscover QIAGEN CLC Genomics ProSuite $565.00 For 50 preps: 50 AllPrep DNA MinElute spin columns, 50 RNeasy Mini spin columns, Collection Tubes, Elution Tubes and Buffers |
The AllPrep PowerFecal DNA/RNA Kit is intended for molecular biology applications. This product is not intended for the diagnosis, prevention, or treatment of a disease.
Product Details
Performance
The AllPrep PowerFecal DNA/RNA Kit efficiently purifies DNA and RNA from stool samples that are inherently rich in PCR inhibitors. Removing such inhibitors is crucial to obtain a good signal in PCR or NGS analysis. The AllPrep PowerFecal DNA/RNA Kit comes with the patented Inhibitor Removal Technology (IRT) ensuring complete removal of inhibitory substances from digested food, heme from lysed red blood cells in stool and other PCR inhibitors (see figure “Inhibitor removal”). The AllPrep PowerFecal DNA/RNA Kit uses improved Microbial Lysis Tubes to ensure high yields of both DNA and RNA from stool samples. The DNA and RNA are suitable for NGS analysis such as microbial operational taxonomic unit (OTU) analysis or comparative metagenomic and metatranscriptomic studies (see figure “OTU analysis with different stool samples”).
Principle
The AllPrep PowerFecal DNA/RNA Kit is designed for simultaneous purification of genomic DNA and total RNA from stool samples. Pure DNA and RNA are obtained from the entire sample, in contrast to other procedures where the biological sample is divided into two before being processed separately. Simply dividing a sample in half for separate DNA and RNA purification procedures, results in the purification of DNA and RNA from different populations of cells, introducing a bias in any downstream analysis.
Procedure
A simple workflow allows the purification of high-quality DNA and RNA from the same stool sample (see flowchart “AllPrep PowerFecal DNA/RNA Kit procedure”).Stool samples are first homogenized followed by a combined chemical and mechanical lysis using prefilled lysis tubes. Inhibitors commonly present in stool samples are then removed before isolation of nucleic acids. Genomic DNA is first immobilized on a silica membrane while RNA molecules pass through. This flow-through is applied to a different column to specifically bind RNA. The bound DNA and RNA, respectively, are further washed and eluted to yield the different nucleic acids in separate eluates. Optional on-column DNase and RNase digestion can further eliminate any traces of cross-contaminating nucleic acids, if required.
Applications
The AllPrep PowerFecal DNA/RNA Kit is optimized to remove inhibitors and lyse microbial cells efficiently by using the patented IRT technology and bead-beating steps, respectively. The DNA and RNA obtained with the AllPrep PowerFecal DNA/RNA Kit are suitable for use in NGS enabling applications such as 16S rDNA profiling, metagenomic shotgun sequencing and metatranscriptomic profiling. As DNA and RNA are isolated in parallel from the same sample, the AllPrep PowerFecal DNA/RNA Kit is ideal for comparative metagenomic and metatranscriptomic analysis.
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品牌介绍
QIAGEN是一家专业化致力于生物分子样品制备解决方案的跨国经营企业，总部位于德国。1984年，QIAGEN在德国成立，1996年在美国纽约纳斯达克上市。QIAGEN拥有超过1000项专利和认可证明，在18个国家设立了分公司，代理商服务国超过40个，在全球有超过400000的用户。QIAGEN提供的产品超过500类，包括各种试剂，耗材和自动化纯化工作站。这些产品用于样品采集，稳定，核酸或蛋白的分离，纯化和检测中，不仅广泛的应用于科研领域的各个方面，在生物技术，制药，法医研究，食品安全检测，畜牧业和分子诊断领域也得到了广泛的应用。QIAGEN产品的卓越品质和在应用中的出色表现使得其成为样品处理中标准的代名词。
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