QIAamp MinElute Virus Vacuum Kit
For simultaneous purification of viral DNA and RNA from plasma, serum, and cell-free body fluids
- Rapid purification of high-quality viral DNA and RNA
- No organic extraction or alcohol precipitation
- Consistent, high yields
- Removal of contaminants and inhibitors
The QIAamp MinElute Virus Vacuum Kit simplifies purification of viral DNA and RNA with fast vacuum procedures. The QIAamp MinElute Virus Vacuum Kit can be used with starting sample volumes up to 0.5ml. The kit combines the selective binding properties of a silica-based membrane with flexible elution volumes of between 20 and 150μl.
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Cat No./ID:57714 QIAamp MinElute Virus Vacuum Kit (50) $347.00 For 50 minipreps: 50 QIAamp MinElute Columns, QIAGEN Protease, carrier RNA, buffers, Extension Tubes (3 ml), Collection Tubes (1.5 ml) |
Product Details
Purified viral nucleic acids are free of proteins, nucleases, and other impurities, and suitable for use in sensitive downstream applications such as PCR and RT-PCR (see figures "High sensitivity in PCR" and "High sensitivity in RT-PCR").
Viral nucleic acids purified using the QIAamp MinElute Virus Vacuum Kit can be used in a wide range of downstream applications, including:
- PCR and quantitative real-time PCR
- Infectious disease research
The QIAamp MinElute Virus Vacuum Kit simplifies isolation of viral RNA and DNA from plasma, serum, and cell-free body fluids with a fast vacuum procedure. No phenol–chloroform extraction is required. Nucleic acids bind specifically to the QIAamp MinElute silica-gel membrane while contaminants pass through. PCR inhibitors, such as divalent cations and proteins, are completely removed in two efficient wash steps, leaving pure nucleic acids to be eluted in either water or a buffer provided with the kit. QIAamp MinElute technology yields viral RNA and DNA from plasma, serum, and cell-free body fluids that are ready to use in PCR and blotting procedures.
The QIAamp MinElute Virus Vacuum Kit combines the selective binding properties of a silica-based membrane with flexible elution volumes of between 20 and 150 µl. QIAamp sample preparation technology is fully licensed.
The QIAamp MinElute Virus Vacuum Kit uses well-established technology for simultaneous purification of viral RNA and DNA. Fresh or frozen plasma, serum, and other cell-free body fluids (500 µl) can be processed in less than an hour.
Specifications
Features | Specifications |
| Applications | PCR, real-time PCR |
| Elution volume | 20–150 µl |
| Format | MinElute columns |
| Main sample type | Serum, plasma |
| Processing | Manual (vacuum) |
| Purification of total RNA, miRNA, poly A+ mRNA, DNA or protein | Viral DNA, viral RNA |
| Sample amount | 500 µl |
| Technology | Silica technology |
| Time per run or per prep | <1> |
| Yield | Varies |
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