AllPrep RNA/Protein Kit
- High-quality RNA and protein from the same sample
- Rapid purification with spin-column–based protocol
- Efficient sample preparation using less cell cultures
- High-performance RNA and native protein
The AllPrep RNA/Protein Kit allows the simultaneous purification of total RNA and native protein from the same cultured cell sample.
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Cat No./ID:80404 AllPrep RNA/Protein Kit (50) $536.00 50 AllPrep Mini Spin Columns, 50 RNeasy Mini Spin Columns, 50 Protein Cleanup Mini Spin Columns, Collection Tubes, RNase-Free Reagents and Buffers |
Product Details
The AllPrep RNA/Protein Kit is designed to purify total RNA and native protein simultaneously from the same sample of cultured cells. For experiments to validate siRNA-mediated knockdown at the mRNA and protein levels, the kit streamlines sample preparation and allows the number of cell cultures to be significantly reduced. In addition, knockdown analysis is more reliable since RNA and protein come from the same sample.The AllPrep RNA/Protein Kit is part of QIAGEN’s range of closely integrated products for RNAi and gene expression analysis. These products provide highly reproducible and sensitive results. Products include optimized PCR master mixes, ready-to-run assays, siRNAs, and transfection reagents. Standardization of RNAi and gene expression analysis workflows by these products have contributed to success in many areas of biomedical research.
The AllPrep RNA/Protein Kit allows purification of RNA and protein from a single cell culture. The purified RNA and protein provide high performance in downstream applications, such as real-time RT-PCR, western blotting, and enzyme assays. (Note: The functionality of any protein of interest in an assay has to be tested according to its properties.)
Specifications
Features | Specifications |
| Applications | PCR, real-time PCR, blotting, microarray |
| Elution volume | 30–50 µl |
| Format | Spin column |
| Main sample type | Cultured cells |
| Processing | Manual (centrifugation) |
| Purification of total RNA, miRNA, poly A+ mRNA, DNA or protein | RNA, native protein |
| Sample amount | 700 µl |
| Technology | Silica technology |
| Time per run or per prep | 40 minutes |
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