Qiagen/Allprep Bacterial DNA/RNA/Protein Kit/For the extraction of protein and nucleic acids from bacterial cell cultures/47054
市场价：
¥10840.00
美元价：
6504.00
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
Allprep Bacterial DNA/RNA/Protein Kit
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For the extraction ofprotein and nucleic acids from bacterial cell cultures
- Efficient solubilization of total nucleic acids and cellular proteins from bacterial samples
- Sequential DNA, RNA and protein extraction in less than 40 minutes
- Ready-to-use, high-quality nucleic acids and proteins for downstream applications
Easy-to-use, the AllPrep Bacterial DNA/RNA/Protein Kit isolates total nucleic acids and cellular proteins from bacterial cultures using spin column technology. Using a bead-based lysis and spin-column collection method, nucleic acids and proteins are effectively extracted from Gram (+/-) bacterial species. DNA and RNA is ready for qPCR, RT-PCR and next-generation sequencing, while protein is ready for 1D SDS-PAGE and mass spectrometry following in-gel trypsin digestion. Some applications, including 2D SDS-PAGE and solution phase proteolytic digestion for mass spectrometry, may require an additional detergent removal step following protein elution.Beginning by lysing cultured bacterial cells using 0.1 mm glass bead tubes, nucleic acids and proteins are made soluble and bound with solution. In three steps of binding and flow through, DNA is bound to one silica spin column and the flow-through containing RNA and proteins is processed through a second and third spin column using buffers designed to assist binding each component. Each spin column is then individually washed and immobilized analyte is eluted.The AllPrep Bacterial DNA/RNA/Protein Kit was previously sold by MO BIO under the same name.
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- Specifications
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Cat No./ID:47054 AllPrep Bacterial DNA/RNA/Protein Kit (50) $542.00 For the extraction ofprotein and nucleic acids from bacterial cell cultures |
The Allprep Bacterial DNA/RNA/Protein Kit is intended for molecular biology applications. This product is not intended for the diagnosis, prevention, or treatment of a disease.
Specifications
Features | Specifications |
| Bead size | 0.1 mm glass |
| Format | Silica Spin Filter |
| Processing | Chemical lysis and/or bead beating |
| Sample size | 1.8 ml of microbial culture QIAGEN recommends using no more than 1x10^9 bacterial cells per sample |
| Sample types | Cultured gram-negative and gram-positive bacteria |
| Storage temperature | Store spin filters at 4°C. All other kit reagents and components can be stored at room temperature (15-30°C). |
| Throughput | 1-24 samples |
| Time per run or per prep | 36 minutes |
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品牌介绍
QIAGEN是一家专业化致力于生物分子样品制备解决方案的跨国经营企业，总部位于德国。1984年，QIAGEN在德国成立，1996年在美国纽约纳斯达克上市。QIAGEN拥有超过1000项专利和认可证明，在18个国家设立了分公司，代理商服务国超过40个，在全球有超过400000的用户。QIAGEN提供的产品超过500类，包括各种试剂，耗材和自动化纯化工作站。这些产品用于样品采集，稳定，核酸或蛋白的分离，纯化和检测中，不仅广泛的应用于科研领域的各个方面，在生物技术，制药，法医研究，食品安全检测，畜牧业和分子诊断领域也得到了广泛的应用。QIAGEN产品的卓越品质和在应用中的出色表现使得其成为样品处理中标准的代名词。
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