QIAamp Viral RNA Mini Kit
- Rapid isolation of high-quality, ready-to-use RNA
- No organic extraction or alcohol precipitation
- Consistent, high yields
- Complete removal of contaminants and inhibitors
The QIAamp Viral RNA Mini Kit simplifies purification of viral RNA from cell-free body fluids with fast spin-column or vacuum procedures. Viral RNA binds specifically to the QIAamp silica membrane, and pure viral RNA is eluted in either water or a buffer provided with the kit. Purification can be fully automated on theQIAcube Connect.
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- Product Details
- Specifications
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Cat No./ID:1020953 Buffer AVE (108 x 2 ml) $477.00 Buffer AVE for elution of RNA |
Cat No./ID:52904 QIAamp Viral RNA Mini Kit (50) $271.00 For 50 RNA preps: 50 QIAamp Mini Spin Columns, carrier RNA, Collection Tubes (2 ml), RNase-free buffers |
Cat No./ID:52906 QIAamp Viral RNA Mini Kit (250) $1,170.00 IMPORTANT: Packaging of this kit is optimized for the global fight against COVID19 caused by the coronavirus (SARS-CoV-2). Only kit format available is 250 prep version. See Important Note for details. For 250 RNA preps: 250 QIAamp Mini Spin Columns, Carrier RNA, Collection Tubes (2 ml), RNase-free buffers |
Product Details
Optimized buffers and enzymes lyse samples, stabilize nucleic acids, and enhance selective RNA adsorption to the QIAamp membrane. To guarantee RNA integrity, samples are lysed under highly denaturing conditions to inactivate RNases. Alcohol is added and lysates loaded onto the QIAamp spin column. Wash buffers are used to remove impurities and pure, ready-to-use RNA is then eluted in water or low-salt buffer.
The QIAamp Viral RNA Mini Kit is automatable on the QIAcube. The QIAamp Viral RNA Mini Accessory Set provides the additional buffers and reagents needed for automated, low-throughput sample prep using both QIAcube and QIAamp Viral RNA Mini Kit.
Vacuum processing
For greater speed and convenience in RNA purification, samples can be processed by vacuum instead of centrifugation. QIAamp Mini spin columns (see figure "QIAamp Viral RNA Mini spin column") are accommodated on the QIAvac 24 manifold using VacValves and VacConnectors, provided in the QIAamp Vac Accessory Set. VacValves should be used if sample flow rates differ significantly, in order to ensure consistent vacuum. Disposable VacConnectors are used to avoid any cross-contamination.
The QIAamp Viral RNA Mini Kit simplifies purification of viral RNA from cell-free body fluids, offering fast spin-column or vacuum procedures, or automation on QIAcube. Sample types include:
- Plasma and serum
- CSF
- Urine
- Other cell-free body fluids
- Cell-culture supernatants
Specifications
Features | Specifications |
| Applications | PCR, qPCR, real-time PCR |
| Elution volume | 50 µl |
| Format | Spin columns |
| Main sample type | Liquid media |
| Processing | Manual (centrifugation or vacuum) |
| Purification of total RNA, miRNA, poly A+ mRNA, DNA or protein | Viral RNA |
| Sample amount | 140 µl |
| Technology | Silica technology |
| Time per run or per prep | 20–40 minutes |
| Yield | >90% recovery |
Product Resources
Product citations in scientific articles
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