Qiagen/RNeasy微型试剂盒/50 RNeasy微型旋转柱、收集管（1.5 ml和2 ml）、无核糖核酸酶试剂和缓冲液
/74104
/74104
市场价：
¥6600.00
美元价：
3960.00
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
RNeasy Mini Kit
Print
For purification of up to 100 µg total RNA from cells, tissues, and yeast
- Fast procedure delivering high-quality total RNA in minutes
- Ready-to-use RNA for high performance in any downstream application
- Consistent RNA yields from small amounts of starting material
- No phenol/chloroform extraction, no CsCl gradients, no LiCl or ethanol precipitation
The RNeasy Mini Kit provides fast purification of high-quality RNA from cells, tissues, and yeast using silica-membrane RNeasy spin columns with a binding capacity of 100 μg RNA. Tissue samples can be conveniently stabilized using RNAprotect TissueReagent or Allprotect Tissue Reagent, and efficiently disrupted using a TissueRuptor or TissueLysersystem. RNA purification using the RNeasy Mini Kit can be fully automated on theQIAcube Connect. For smaller and larger samples, the RNeasy Micro Kit (spin-column binding capacity of up to 45 µg RNA), the RNeasy Midi Kit (spin-column binding capacity of 1 mg RNA), and RNeasy Maxi Kit (spin-column binding capacity of6 mg RNA) are also available.
- Buy Products
- Product Details
- Specifications
Buy Products
Cat No./ID:74104 RNeasy Mini Kit (50) $330.00 50 RNeasy Mini Spin Columns, Collection Tubes (1.5 ml and 2 ml), RNase-free Reagents and Buffers |
Cat No./ID:74106 RNeasy Mini Kit (250) $1,451.00 250 RNeasy Mini Spin Columns, Collection Tubes (1.5 ml and 2 ml), RNase-free Reagents and Buffers |
The RNeasy Mini Kit is intended for molecular biology applications. This product is not intended for the diagnosis, prevention, or treatment of a disease.
Product Details
Performance
Total RNA is easily purified with the RNeasy Mini Kit from up to 30 mg animalor human tissues,from 100to 1x107 animal or human cells, oryeast (see table; and figures "RT-PCR of RNA from as few as 100 cells" and "High-quality RNA from a variety of samples").
| Source | Starting material | Average yield (µg) |
| Animal cells | ||
| LMHHeLaCOS-7Lymphocytes (unstimulated) | 1x 1061x 1061x 1061x 106 | 1215350.5 |
| Mouse tissue | ||
LiverLungSpleen | 10mg10mg10 mg | 401035 |
| Yeast cells | ||
S. cerevisiae | 1x 107 | 25 |
Principle
The RNeasy Mini Kit allows efficient purification of total RNA from small amounts of starting material. RNeasy technology simplifies total RNA isolation by combining the stringency of guanidine-isothiocyanate lysis with the speed and purity of silica-membrane purification. RNeasy Kits provide the highest-quality RNA with minimum copurification of DNA.
Procedure
With the RNeasy Mini Kit, total RNA is easily purified from 10 to 1 x 107 animal or human cells, 0.5–30 mg animal or human tissues, and <5 x 107 yeast cells. Samples are first lysed and then homogenized. Ethanol is added to the lysate to provide ideal binding conditions. The lysate is then loaded onto the RNeasy silica membrane (see figure "RNeasy Mini spin column"). RNA binds (up to 100 µg capacity), and all contaminants are efficiently washed away. For certain RNA applications that are sensitive to very small amounts of DNA, the residual amounts of DNA remaining can be removed using a convenient on-column DNase treatment. Pure, concentrated RNA is eluted in 30–100 µl water (see figure "RNeasy Mini procedure"). A variety of special application protocols is also available.
Applications
RNA purified with RNeasy technology has A260/280 ratios of 1.9–2.1 (measured in 10 mM Tris·Cl, pH 7.5) and is ideal for use in all applications. Downstream applications include:
- Northern, dot, and slot blotting
- End-point RT-PCR
- Quantitative, real-time RT-PCR
- Array analysis
- Poly A+ RNA selection
Specifications
Features | Specifications |
| Applications | Northern, dot, and slot blotting, end-point RT-PCR, quantitative, real-time RT-PCR, array analysis, next-generation sequencing |
| Elution volume | 30–100 µl |
| Format | Spin column |
| Main sample type | Tissue, cells |
| Processing | Manual (centrifugation) |
| Purification of total RNA, miRNA, poly A+ mRNA, DNA or protein | Total RNA |
| Sample amount | 0.5–30 mg |
| Technology | Silica technology |
| Time per run or per prep | 20 minutes |
| Yield | Varies |
Product Resources
Product citations in scientific articles
Powered by Bioz
See more details on BiozCustomers who bought these products also bought
- Cat No./ID:74134
RNeasy Plus Mini Kit (50)
For 50 minipreps: RNeasy Mini Spin Columns, gDNA Eliminator Spin Columns, Collection Tubes, RNase-Free Water and Buffers$374.00Add To Cart - Cat No./ID:74136
RNeasy Plus Mini Kit (250)
For 250 minipreps: RNeasy Mini Spin Columns, gDNA Eliminator Spin Columns, Collection Tubes, RNase-Free Water and Buffers$1,637.00Add To Cart - Cat No./ID:74034
RNeasy Plus Micro Kit (50)
For 50 micropreps: RNeasy MinElute Spin Columns, gDNA Eliminator Spin Columns, Collection Tubes, Carrier RNA, RNase-Free Water and Buffers$462.00Add To Cart - Cat No./ID:79254
RNase-Free DNase Set (50)
1500 Kunitz units RNase-free DNase I, RNase-free Buffer RDD, and RNase-free water for 50 RNA minipreps$113.00Add To Cart - Cat No./ID:79656
QIAshredder (250)
250 disposable cell-lysate homogenizers for use in nucleic acid minipreps, caps$406.00Add To Cart - Cat No./ID:79654
QIAshredder (50)
50 disposable cell-lysate homogenizers for use in nucleic acid minipreps, caps$93.30Add To Cart - Cat No./ID:79256
RNase-Free DNase Set (250)
5 x 1500 Kunitz units RNase-free DNase I, RNase-free Buffer RDD, and RNase-free water for 250 RNA minipreps$512.00Add To Cart - Cat No./ID:28104
QIAquick PCR Purification Kit (50)
For purification of 50 PCR reactions: 50 QIAquick Spin Columns, Buffers, Collection Tubes (2 ml)$123.00Add To Cart - Cat No./ID:28704
QIAquick Gel Extraction Kit (50)
For gel extraction or cleanup of 50 reactions: 50 QIAquick Spin Columns, Buffers, Collection Tubes (2 ml)$124.00Add To Cart - Cat No./ID:69506
DNeasy Blood & Tissue Kit (250)
250 DNeasy Mini Spin Columns, Proteinase K, Buffers, Collection Tubes (2 ml)$736.00Add To Cart - Cat No./ID:69504
DNeasy Blood & Tissue Kit (50)
50 DNeasy Mini Spin Columns, Proteinase K, Buffers, Collection Tubes (2 ml)$171.00Add To Cart - Cat No./ID:74004
RNeasy Micro Kit (50)
50 RNeasy MinElute Spin Columns, Collection Tubes (1.5ml and 2ml), RNase-free DNase I, Carrier RNA, RNase-free Reagents and Buffers$522.00Add To Cart - Cat No./ID:27104
QIAprep Spin Miniprep Kit (50)
For 50 high-purity plasmid minipreps: 50 QIAprep 2.0 Spin Columns, Reagents, Buffers, Collection Tubes (2 ml)$96.90Add To Cart - Cat No./ID:27106
QIAprep Spin Miniprep Kit (250)
For 250 high-purity plasmid minipreps: 250 QIAprep 2.0 Spin Columns, Reagents, Buffers, Collection Tubes (2 ml)$422.00Add To Cart - Cat No./ID:28106
QIAquick PCR Purification Kit (250)
For purification of 250 PCR reactions: 250 QIAquick Spin Columns, Buffers, Collection Tubes (2 ml)$561.00Add To Cart - Cat No./ID:205311
QuantiTect Rev. Transcription Kit (50)
For 50 x 20 µl reactions: 100 µl 7x gDNA Wipeout Buffer, 50 µl Quantiscript Reverse Transcriptase, 200 µl 5x Quantiscript RT Buffer, 50 µl RT Primer Mix, 1.9 ml RNase-Free Water$391.00Add To Cart - Cat No./ID:51306
QIAamp DNA Mini Kit (250)
For 250 DNA preps: 250 QIAamp Mini Spin Columns, QIAGEN Proteinase K, Reagents, Buffers, Collection Tubes (2 ml)$757.00Add To Cart - Cat No./ID:12143
QIAGEN Plasmid Midi Kit (25)
25 QIAGEN-tip 100, Reagents, Buffers$303.00Add To Cart - Cat No./ID:205313
QuantiTect Rev. Transcription Kit (200)
For 200 x 20 µl reactions: 4 x 100 µl 7x gDNA Wipeout Buffer, 4 x 50 µl Quantiscript Reverse Transcriptase, 4 x 200 µl 5x Quantiscript RT Buffer, 4 x 50 µl RT Primer Mix, 4 x 1.9 ml RNase-Free Water$1,300.00Add To Cart - Cat No./ID:79306
QIAzol Lysis Reagent (200ml)
200 ml QIAzol Lysis Reagent$336.00Add To Cart
品牌介绍
QIAGEN是一家专业化致力于生物分子样品制备解决方案的跨国经营企业，总部位于德国。1984年，QIAGEN在德国成立，1996年在美国纽约纳斯达克上市。QIAGEN拥有超过1000项专利和认可证明，在18个国家设立了分公司，代理商服务国超过40个，在全球有超过400000的用户。QIAGEN提供的产品超过500类，包括各种试剂，耗材和自动化纯化工作站。这些产品用于样品采集，稳定，核酸或蛋白的分离，纯化和检测中，不仅广泛的应用于科研领域的各个方面，在生物技术，制药，法医研究，食品安全检测，畜牧业和分子诊断领域也得到了广泛的应用。QIAGEN产品的卓越品质和在应用中的出色表现使得其成为样品处理中标准的代名词。
联络我们
