RNeasy Micro Kit
- Fast procedure delivering high-quality total RNA in minutes
- Ready-to-use RNA for high performance in any downstream application
- Consistent RNA yields from very small amounts of starting material
- No phenol/chloroform extraction
- No CsCl gradients, no LiCl or ethanol precipitation
The RNeasy Micro Kit is designed for purification of up to 45 μg RNA from small cell and tissue samples. Tissue samples can be conveniently stabilized using RNAprotect TissueReagentor Allprotect Tissue Reagent, and efficiently disrupted using a TissueRuptor or TissueLysersystem. RNA purification using the RNeasy Micro Kit can be fullyautomated on theQIAcube Connect.For larger samples, the RNeasy Mini Kit (spin-column binding capacity of 100 µg RNA), RNeasy Midi Kit (spin-column binding capacity of 1 mg RNA), and RNeasy Maxi Kit (spin-column binding capacity of 6 mg RNA) are also available.
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Cat No./ID:74004 RNeasy Micro Kit (50) $522.00 50 RNeasy MinElute Spin Columns, Collection Tubes (1.5ml and 2ml), RNase-free DNase I, Carrier RNA, RNase-free Reagents and Buffers |
Product Details
The RNeasy Micro Kit is designed for isolation of total RNA from small samples such as microdissected tissues and fine needle aspirates, from milligram amounts of fibrous tissues including heart and muscle tissue, and from small numbers of cells down to single cells (e.g., FACS sorted cells). For microdissected FFPE tissues, we recommend the RNeasy FFPE Kit. RNeasy technology simplifies total RNA isolation by combining the stringency of guanidine-isothiocyanate lysis with the speed and purity of silica-membrane purification and the unique design of RNeasy MinElute spin columns enables minimal elution volumes, as little as 10 µl, to concentrate the RNA. The small elution volume means that reaction volumes can be kept small in downstream applications, giving increased reaction efficiency. The high purity allows the complete sample to be used in downstream reactions, such as reverse transcription, without enzyme inhibition.
The RNeasy Micro Kit is designed for isolation of total RNA from small samples. Samples (<5 mg tissue, or <5 x 105 cells) are first lysed and then homogenized. Ethanol is added to the lysate to provide ideal binding conditions. The lysate is then loaded onto the RNeasy MinElute spin column and RNA (up to 45 µg) binds to the silica membrane. RNase-free DNase, provided in the RNeasy Micro Kit, enables simple and efficient on-column digestion of genomic DNA for sensitive applications. Then DNase and any contaminants are efficiently washed away, and pure, concentrated RNA is eluted in 10–14 µl water.
Highly reproducible isolation of RNA in a small elution volume (10µl) using the RNeasy Micro Kit makes the highly pure RNA well-suited for sensitive quantitative gene expression analyses, including real-time RT-PCR starting with as little as one cell.
Specifications
Features | Specifications |
| Applications | Northern, dot, and slot blotting, end-point RT-PCR, quantitative, real-time RT-PCR, array analysis, next-generation sequencing |
| Elution volume | 10–14 µl |
| Format | Spin column |
| Main sample type | Tissue, cells |
| Processing | Manual |
| Purification of total RNA, miRNA, poly A+ mRNA, DNA or protein | RNA |
| Sample amount | <5> |
| Technology | Silica technology |
| Time per run or per prep | 30–40 minutes |
| Yield | Varies |
Product Resources
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