Qiagen/RNeasy 96 QIAcube HT套件/用于480个预处理：RNeasy 96板、无核糖核酸酶水、缓冲液/74171
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¥27680.00
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16608.00
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3392242852
电话号码：
4000-520-616
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商品介绍
RNeasy 96 QIAcube HT Kit
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For automated high-throughput isolation of RNAfrom animal and human cells and tissue
- Simple and reliable automated processing for time and cost savings
- Flexible procedures for isolating total RNA or total RNA, including miRNA
- Protocols for various starting materials and desired size distribution
- High-quality purified RNA ready for use in any downstream application
- Consistent RNA yields from small amounts of starting material
The RNeasy 96 QIAcube HT Kit enables simple, automated purification of total RNA or total RNA, including miRNA, from animal and human cells and tissue samples on the QIAcube HTsystem. Using proven RNeasy silica-membrane technology in a convenient 96-well format, contaminants and inhibitors are removed to yield high-quality RNA ready for downstream analysis.
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- Product Details
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Cat No./ID:74171 RNeasy 96 QIAcube HT Kit (5) $1,384.00 For 480 preps: RNeasy 96 plates, RNase-free water, buffers |
Cat No./ID:950067 QIAcube HT Plasticware $321.00 Plasticware for 480 typical preps on QIAcube HT: 5S-Blocks, 5 Elution Microtubes RS (EMTR), 120x8-Well Strip Caps for EMTR, 9 x 96 Filter-Tips OnCor C, TapePad |
The RNeasy 96 QIAcube HT Kit is intended for molecular biology applications. This product is not intended for the diagnosis, prevention, or treatment of a disease.
Product Details
Performance
Purification of RNA on QIAcube HT is simple and reliable with the RNeasy 96 QIAcube HT Kit and provides linear sample recovery with no significant well-to-well variation across a 96-well plate (see figureLinear RNA recovery and Reproducible 96-well RNA purification). This economical, easy-to-use system provides the same results as other trusted QIAGEN purification technologies (see figure Automated purification of RNA from tissues). Analysis of QIAcube HT purified samplesshows high RNA Integrity Scores (RIS) when analyzed with the QIAxcel Advanced system, andperformance similar to other automated QIAGEN purification solutions (see figure High-quality RNA from tissues).The RNeasy 96 QIAcube HT Kit provides consistent yields from small amounts of starting sample material (see table Typical total RNA yields from cells with the RNeasy 96 QIAcube HT Kit).
| Cell line | Source | RNA yield (µg per 105 cells)* |
|---|---|---|
| HeLa | Human cervical carcinoma | 1.6 |
| LMH | Chicken hepatoma | 1.3 |
| COS-7 | Monkey kidney, SV-40 transformed | 3.1 |
| Huh7 | Human hepatoma | 2.0 |
| Jurkat | Human T-cell leukemia | 1.4 |
| K-562 | Human chronic myelogenous leukemia in blast crisis | 1.9 |
Principle
The RNeasy 96 QIAcube HT Kitenables automated purification of total RNA or total RNA, including miRNA, from 5 x 105 animal or human cells or <40 mg tissue using the QIAcube HT instrument.Six instrument protocols are available, depending on the starting material and desired size distribution of purified RNA. Cell samples can be purified directly using the RNeasy 96 QIAcube HT Kit and the dedicated kit protocol. Tissue samples (<40 mg) and samples for purification of small RNA are processed following sample lysis in QIAzol Lysis Reagent. The procedures yield high-qualityRNA that performs well in downstream applications. The RNeasy 96 QIAcube HT Kit combines the selective binding properties of a silica-based membrane with a high-throughput 96-well format, and is designed for fully-automated, simultaneous processing of 24–96 samples on the QIAcube HT instrument.
| Specification | Description |
|---|---|
| Number of samples | 24–96 samples (to be processed in increments of 8) |
| Sample input volume | Up to 5 x 105 cells, homogenized in 140 μl Buffer RLT |
| Elutionvolume | 110μl |
| Duration | 96 samples in approximately 93 or 114* minutes 24 samples in approximately 54 or 73* minutes |
| Specification | Description |
|---|---|
| Number of samples | 24–96 samples (to be processed in increments of 8) |
| Sample input volume | 350 μl aqueous phase from up to 40 mg frozen or 20 mgstabilized tissue homogenized in 750 μl QIAzol Lysis Reagent |
| Elutionvolume | 110μl |
| Duration | 96 samples in approximately 73 or 93* minutes 24 samples in approximately 34 or 54* minutes |
| Specification | Description |
|---|---|
| Number of samples | 24–96 samples (to be processed in increments of 8) |
| Sample input volume | 350 μl aqueous phase from up to 40 mg frozen or 20 mgstabilized tissue homogenized in 750 μl QIAzol Lysis Reagent |
| Elutionvolume | 110μl |
| Duration | 96 samples in approximately78 minutes 24 samples in approximately38 minutes |
Procedure
Cell lysis is performed manually under highly denaturing conditions with guanidine thiocyanate to immediately inactivate RNases and ensure purification of intact RNA. After the run is started, ethanol is added to the lysate to provide appropriate binding conditions, and the samples are then applied to the wells of the RNeasy 96 plate. Total RNA binds and contaminants are efficiently washed away. High-quality RNA is then eluted in a small volume of RNase-free water, ready for use in any downstream application.With the automated procedure for purification of total RNA from animal or human cells, all RNA molecules longer than 200 nucleotides are purified. This provides enrichment for mRNA, since most RNAs <200 nucleotides (such as 5.8S rRNA, 5S rRNA, and tRNA, which together comprise 15–20% of total RNA) are selectively excluded. The size distribution of the purified RNA is comparable to that obtained by centrifugation through a CsCl cushion, where small RNA does not sediment efficiently.For purification of total RNA from tissue, or of total RNA including small RNA, from cells and tissues, we recommend first using QIAzol Lysis Reagent as the lysis buffer. Detailed procedures are provided in the kit handbook.
Applications
The RNeasy 96 QIAcube HT Kit provides efficient, high-throughput RNA sample preparation for research use in fields such as drug screening and basic research. The purified RNA is ready to use in any downstream application, including:
- RT-PCR and qRT-PCR
- Differential display
- cDNA synthesis
- Northern, dot, and slot blot analyses
- Primer extension
- Poly A+ RNA selection
- RNase/S1 nuclease protection
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品牌介绍
QIAGEN是一家专业化致力于生物分子样品制备解决方案的跨国经营企业，总部位于德国。1984年，QIAGEN在德国成立，1996年在美国纽约纳斯达克上市。QIAGEN拥有超过1000项专利和认可证明，在18个国家设立了分公司，代理商服务国超过40个，在全球有超过400000的用户。QIAGEN提供的产品超过500类，包括各种试剂，耗材和自动化纯化工作站。这些产品用于样品采集，稳定，核酸或蛋白的分离，纯化和检测中，不仅广泛的应用于科研领域的各个方面，在生物技术，制药，法医研究，食品安全检测，畜牧业和分子诊断领域也得到了广泛的应用。QIAGEN产品的卓越品质和在应用中的出色表现使得其成为样品处理中标准的代名词。
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