Qiagen/miRNeasy Serum/Plasma Advanced Kit/For 50 total RNA preps: 50 RNeasy MinElute Spin Columns, Collection Tubes (1.5 ml and 2 ml), RNase-Free Reagents and Buffers/217204
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商品介绍
miRNeasy Serum/Plasma Advanced Kit
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For phenol-free isolation of total RNA including miRNA from serum and plasma samples
- Phenol-free protocol – no need for phase separation or working under the hood
- MinElute columns allow for small elution volumes (approx.20 µl)
- Consistent, pureRNA for all downstream applications
The miRNeasy Serum/Plasma Advanced Kit enables the isolation of total RNA, including miRNA, from a minimum of 200 µl of sample. The phenol-free protocol allows all steps to be performed on the bench and removes the need for phase separation, making the kit suitable for automation, e.g., on the QIAcube Connect. The kit includes UCP columns that go through a rigorous ultraclean production process to ensure highly pure RNA eluates.
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Cat No./ID:217204 miRNeasy Serum/Plasma Advanced Kit (50) $461.00 For 50 total RNA preps: 50 RNeasy MinElute Spin Columns, Collection Tubes (1.5 ml and 2 ml), RNase-Free Reagents and Buffers |
The miRNeasy Serum/Plasma Advanced Kit is intended for molecular biology applications. This product is not intended for the diagnosis, prevention, or treatment of a disease.
Product Details
Performance
The miRNeasy Serum/Plasma Advanced Kit provides a phenol-free alternative to the miRNeasy Serum/Plasma Kit with similar RNA yields and quality (see figure High recovery of miRNA without the need for phenol). The miRNeasy Serum/Plasma Advanced Kit efficiently isolates total RNA, including miRNA, from serum and plasma samples starting from a minimum input amount of 200 µl. The phenol-free protocol uses easy-to-automate MinElute spin column technology without the need for a vacuum pump (see figure miRNeasy Serum/Plasma Advanced Kit procedure). Extracellular vesicles are lysed to allow analysis of RNA in and outside of vesicles (see figure Detection of miRNAs preferentially found inside and outside of extracellular vesicles). Unlike kits from other suppliers, the miRNeasy Serum/Plasma Advanced Kit combines ease-of-use and a phenol-free protocol without any compromise on RNA quality or yield (see figure Competitive yields and performance).
Principle
Foranalysis of the complete plasma RNA content, including RNA contained within extracellular vesicles, Buffer RPL mixed with the plasma sampleis used to ensureefficient lysis. The kit includes Buffer RPP, which precipitates contaminants such as plasma proteins. The kit employs a protocol based on spin-column technology. The sample is first applied to the spin column to bind total RNA and any unwanted analytes or contaminants are removed in subsequent wash and centrifugation steps before the sample is eluted in the final centrifugation step.
Procedure
Serum or plasma (200 µl) are mixed with Buffer RPL to release and stabilize RNA from plasma proteins and extracellular vesicles. The sample is then mixed with Buffer RPP and centrifuged to precipitate proteins. Isopropanol is added to the supernatant to provide the appropriate conditions for RNA molecules (>18 nucleotides) to bind to the silica membrane. The sample is then applied to the RNeasy UCP MinElute spin column, where RNA binds to the membrane and other contaminants are washed away in subsequent wash steps. In the final step, total RNA (>18 nucleotides) is eluted using RNase-free water.
Applications
The miRNeasy Serum/Plasma Advanced Kit isolates total cell-free and exosomal RNA from plasma or serum. Downstream applications include:
- Biomarker discovery
- Expression profiling
- Next-generation sequencing
- Quantitative, real-time RT-PCR
- Microarray analysis
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品牌介绍
QIAGEN是一家专业化致力于生物分子样品制备解决方案的跨国经营企业，总部位于德国。1984年，QIAGEN在德国成立，1996年在美国纽约纳斯达克上市。QIAGEN拥有超过1000项专利和认可证明，在18个国家设立了分公司，代理商服务国超过40个，在全球有超过400000的用户。QIAGEN提供的产品超过500类，包括各种试剂，耗材和自动化纯化工作站。这些产品用于样品采集，稳定，核酸或蛋白的分离，纯化和检测中，不仅广泛的应用于科研领域的各个方面，在生物技术，制药，法医研究，食品安全检测，畜牧业和分子诊断领域也得到了广泛的应用。QIAGEN产品的卓越品质和在应用中的出色表现使得其成为样品处理中标准的代名词。
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