Qiagen/RNeasy Lipid Tissue Mini Kit/50 RNeasy Mini Spin Columns, Collection Tubes (1.5 ml and 2 ml), QIAzol Lysis Reagent, RNase-free Reagents and Buffers/74804
市场价：
¥8500.00
美元价：
5100.00
联系Q Q：
3392242852
电话号码：
4000-520-616
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info@ebiomall.com
商品介绍
RNeasy Lipid Tissue Mini Kit
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For purification of up to 100 µg total RNA from fatty tissues and other types of tissue
- Optimized lysis conditions for fatty tissues and other tissues
- High yields of total RNA without phenol contamination
- High-quality RNA for all downstream applications
The RNeasy Lipid Tissue Mini Kit includes QIAzol Lysis Reagent for lysing fatty tissues and other types of tissue, and RNeasy spin columns for purifying up to 100 µg of high-quality RNA. The kit can be automated using theQIAcube Connect. Tissue samples can be conveniently stabilized using RNAprotect TissueReagent (nonfatty tissues only) orAllprotect Tissue Reagent, and efficiently disrupted using a TissueRuptor or TissueLysersystem. For larger samples, the RNeasy Lipid Tissue Midi Kit (spin-column binding capacity of 1 mg RNA) is also available.
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Cat No./ID:74804 RNeasy Lipid Tissue Mini Kit (50) $425.00 50 RNeasy Mini Spin Columns, Collection Tubes (1.5 ml and 2 ml), QIAzol Lysis Reagent, RNase-free Reagents and Buffers |
The RNeasy Lipid Tissue Mini Kit is intended for molecular biology applications. This product is not intended for the diagnosis, prevention, or treatment of a disease.
Product Details
Performance
The RNeasy Lipid Tissue protocol integrates QIAzol lysis with RNeasy RNA isolation for high yields of total RNA from fatty tissues with an average yield of RNA from 10 mg of brain of 10 µg, and 2.4 µg of RNA from a 10 mg sample of adipose tissue. RNeasy Lipid Tissue kits provide efficient isolation of high-quality total RNA without phenol carryover (see figure "High yields of RNA without phenol carryover"). The RNA from fatty tissues is suitable for downstream applications including real-time RT-PCR (see figure "Real-time analysis of high-quality RNA").
Principle
RNeasy Lipid Tissue Kits are optimized for use with fatty tissues, such as brain and adipose tissue. The convenient RNeasy Lipid Tissue protocol integrates optimized phenol/guanidine-based lysis with proven RNeasy purification for isolation of high yields of high-quality total RNA. The combination of organic extraction and chaotropic disruption contributes to the high lysis efficiency of QIAzol Lysis Reagent. Since the RNeasy procedures enrich for mRNA and other RNA species >200 nucleotides, the total RNA yield does not include 5S rRNA, tRNA, and other low-molecular-weight RNAs, which make up 15-20% of total cellular RNA.
Procedure
Tissue samples 10–100 mg are homogenized in QIAzol Lysis Reagent. After addition of chloroform, the homogenate is separated into aqueous and organic phases by centrifugation. The upper, aqueous phase is extracted, and ethanol is added to provide appropriate binding conditions. The sample is then applied to the RNeasy spin column, where the total RNA (up to 100 µg) binds to the membrane and phenol and other contaminants are efficiently washed away. High-quality RNA is then eluted in 30–100 µl of RNase-free water water (see figure "RNeasy Lipid Tissue Mini procedure").
Applications
RNeasy Lipid Tissue Kits provide easy and efficient isolation of high-quality RNA for all downstream applications, including array analysis and real-time RT-PCR.
Specifications
Features | Specifications |
| Applications | PCR, real-time PCR, microarray |
| Elution volume | 30–100 µl |
| Format | Spin column |
| Main sample type | Fatty tissue samples |
| Processing | Manual |
| Purification of total RNA, miRNA, poly A+ mRNA, DNA or protein | RNA |
| Sample amount | 10–100 mg |
| Technology | Silica technology |
| Time per run or per prep | 45 minutes |
| Yield | 2.4–10 mg |
Product Resources
Product citations in scientific articles
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品牌介绍
QIAGEN是一家专业化致力于生物分子样品制备解决方案的跨国经营企业，总部位于德国。1984年，QIAGEN在德国成立，1996年在美国纽约纳斯达克上市。QIAGEN拥有超过1000项专利和认可证明，在18个国家设立了分公司，代理商服务国超过40个，在全球有超过400000的用户。QIAGEN提供的产品超过500类，包括各种试剂，耗材和自动化纯化工作站。这些产品用于样品采集，稳定，核酸或蛋白的分离，纯化和检测中，不仅广泛的应用于科研领域的各个方面，在生物技术，制药，法医研究，食品安全检测，畜牧业和分子诊断领域也得到了广泛的应用。QIAGEN产品的卓越品质和在应用中的出色表现使得其成为样品处理中标准的代名词。
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