RNeasy Plus Universal Kits
- One-for-all tissue solution
- Time-saving integration of RNeasy and QIAzol technologies
- Fast non-enzymatic removal of genomic DNA
- Pure RNA for use in downstream applications
RNeasy Plus Universal Kits integrate fast, convenient RNA purification with effective elimination of genomic DNA. Optimized protocols enable purification of high-quality RNAfrom any type of tissue, even difficult-to-lyse tissues. The kits include QIAzol Lysis Reagent for lysing fatty tissue and other types of tissue and RNeasy spin columns for purifying high-quality RNA. Kits are available intwo formats: the RNeasy Plus Universal Mini Kit enables purification of RNA from up to 50 mg tissue and the RNeasy Plus Universal Midi Kit enables purification of RNA from up to 250 mg tissue. The RNeasy Plus Universal Mini Kit can be automated on theQIAcube Connect.
- Buy Products
- Product Details
- Specifications
Buy Products
Cat No./ID:73404 RNeasy Plus Universal Mini Kit (50) $431.00 For 50 RNA minipreps: RNeasy Mini Spin Columns, gDNA Eliminator Solution, Collection Tubes, RNase-Free Water and Buffers |
Cat No./ID:73442 RNeasy Plus Universal Midi Kit (10) $224.00 For 10 RNA midipreps: RNeasy Midi Spin Columns, gDNA Eliminator Solution, Collection Tubes, RNase-Free Water and Buffers |
Product Details
The RNeasy Plus Universal procedure allows high, reproducible RNA yields from any type of tissue, including fatty tissues and difficult-to-lyse tissue (see table). Yields can vary due to factors such as species and developmental stage, especially with adipose tissues.
| Mouse/rat tissue (10 mg) | Yield of total RNA ((µg) |
|---|---|
| Adipose tissue | 0.5–2.5 |
| Brain | 5–20 |
| Heart | 5–25 |
| Intestine | 10–60 |
| Kidney | 5–40 |
| Liver | 15–80 |
| Lung | 5–15 |
| Muscle | 5–35 |
| Skin | 2–5 |
| Spleen | 15–100 |
The RNeasy Plus Universal procedure enables purification of RNA from any type of tissue, including fatty or difficult-to-lyse tissues. RNeasy Plus Universal Kits integrate phenol/guanidine-based sample lysis and silica-membrane purification of total RNA. QIAzol Lysis Reagent, included in the kits, is a monophasic solution of phenol and guanidine thiocyanate, designed to facilitate lysis of all kinds of tissues and to inhibit RNases. The highly efficient lysis and the subsequent removal of contaminants by organic phase extraction enable the use of larger amounts of tissue with RNeasy spin columns. gDNA Eliminator solution effectively removes genomic DNA contamination and significantly improves separation of DNA into the interphase.
The RNeasy Plus Universal procedure is easy to follow and, compared to phenol/chloroform treatment with a subsequent cleanup procedure, much faster and more efficient with better RNA purity and yield (see flowchart "RNeasy Plus Universal Mini and Midi procedures"). Total RNA is purified from up to 50 mg tissue using the RNeasy Plus Universal Mini Kit or up to 250 mg tissue using the RNeasy Plus Midi Kit.
Tissue samples are homogenized in QIAzol Lysis Reagent using the TissueRuptor, TissueLyser LT, or TissueLyser II. After addition of gDNA Eliminator Solution and chloroform, the homogenate is separated into aqueous and organic phases by centrifugation. The upper, aqueous phase with RNA is collected, mixed with ethanol, and RNA is purified using RNeasy spin columns. Total RNA binds to the spin column membrane, and phenol and other contaminants are efficiently washed away. High-quality RNA is eluted in RNase-free water.
The procedure provides an enrichment for mRNA since most RNAs <200 nucleotides (such as rRNAs and tRNAs) are excluded. The RNeasy Plus Universal procedure can be modified to allow the purification of total RNA containing small RNAs, such as miRNA, from cultured cells.
RNA purified using the RNeasy Plus Universal Kits is ideally suited for downstream applications that are sensitive to low amounts of DNA contamination, such as quantitative real-time RT-PCR. The purified RNA can also be used in other applications.
Specifications
Features | Specifications |
| Applications | Northern, dot, and slot blotting, end-point RT-PCR, quantitative, real-time RT-PCR, array analysis, next-generation sequencing |
| Elution volume | Mini: 30µl; Midi:150µl |
| For automated processing | Mini format can be automated on the QIAcube |
| Format | Mini and Midi Format |
| Integrated removal of genomic DNA | Yes |
| Main sample type | Tissue samples including difficult-to-lyse and demanding tissues |
| Processing | Manual (centrifugation), automated (QIAcube) |
| Purification of total RNA, miRNA, poly A+ mRNA, DNA or protein | RNA |
| Sample amount | Mini: 5-50 mg; Midi: 50-250 mg |
| Technology | QIAzol/Spin column technology |
Product Resources
Customers who bought these products also bought
- Cat No./ID:208352
QuantiNova Probe RT-PCR Kit (100)
For 100 x 20 µl reactions: 1 ml QuantiNova Probe RT-PCR Master Mix, 20 µl QuantiNova Probe RT Mix, 20 µl Internal Control RNA, 500 µl Yellow Template Dilution Buffer, 250 µl ROX Reference Dye, 1.9 µl RNase-Free Water$170.00Add To Cart - Cat No./ID:208552
QuantiNova Multiplex RT-PCR Kit (100)
For 100 x 20 µl reactions: 0.5 ml 4x QuantiNova Multiplex RT-PCR Master Mix, 20 µl QuantiNova Multiplex RT-Mix, 20 µl QuantiNova IC RNA, 500 µl QuantiNova Yellow Template Dilution Buffer, 250 µl QN ROX Reference Dye, 1.9 ml RNase-Free Water$221.00Add To Cart - Cat No./ID:85600
TissueLyser LT
Compact bead mill, 100–240 V AC, 50–60 Hz; requires the TissueLyser LT Adapter, 12-Tube (available separately)$6,110.00Add To Cart - Cat No./ID:13114-325
Ceramic Beads, 2.8 mm (325 g)
Customize your bead tubes with bulk beads for rapid and reliable biological sample lysis from a wide variety of starting materials.$264.00Add To Cart - Cat No./ID:9243592
Tip Disposal Box Holder
Holds the tip disposal box in place and ensures correct positioning under the tip chute; suitable for QIAcube HT, QIAxtractor, and QIAgility instruments$221.00Add To Cart - Cat No./ID:69989
Stainless Steel Beads, 5 mm (200)
200 stainless steel beads (5 mm diameter), suitable for use with TissueLyser systems$208.00Add To Cart - Cat No./ID:216213
FastLane Cell SYBR Green Kit (200)
FastLane Cell One-Step Buffer Set, 2x QuantiTect SYBR Green RT-PCR Master Mix, and QuantiTect RT Mix$1,864.00Add To Cart - Cat No./ID:12866-25
RNeasy PowerSoil Total RNA Kit
For the isolation of high quality total RNA from all soil types$362.00Add To Cart - Cat No./ID:339459
miRCURY LNA miRNA ISH Buffer and Controls
Scramble miRNA Negative Control Probe, U6 snRNA Positive Control Probe, 2x Formamide-free miRNA ISH buffer, Proteinase K; for 400 slides$1,490.00Add To Cart - Cat No./ID:79306
QIAzol Lysis Reagent (200ml)
200 ml QIAzol Lysis Reagent$336.00Add To Cart - Cat No./ID:239025
GelPilot 50 bp Ladder (100)
Ready-to-use DNA marker; 600 µl (100 lanes), 9 fragments: 50–500 bp$176.00Add To Cart - Cat No./ID:76106
RNAprotect Tissue Reagent (250ml)
250 ml RNAprotect TissueReagent for stabilization of RNA in 125 x 200 mg tissue samples
$369.00Add To Cart - Cat No./ID:239085
GelPilot 1 kb Ladder (100)
Ready-to-use DNA marker; 600 µl (100 lanes), 9 fragments: 1000–10,000 bp$137.00Add To Cart - Cat No./ID:74134
RNeasy Plus Mini Kit (50)
For 50 minipreps: RNeasy Mini Spin Columns, gDNA Eliminator Spin Columns, Collection Tubes, RNase-Free Water and Buffers$374.00Add To Cart - Cat No./ID:929551
QX DNA Size Marker FX174/HaeIII (50 ul)
DNA size marker with fragments of 72, 118, 194, 234, 271, 281, 310, 603, 872, 1078, and 1353 bp; concentration 100 ng/ul$83.80Add To Cart - Cat No./ID:76104
RNAprotect Tissue Reagent (50ml)
50 ml RNAprotect Tissue Reagent for stabilization of RNA in 25 x 200 mg tissue samples
$88.60Add To Cart - Cat No./ID:69514
DNeasy mericon Food Kit (50)
50 QIAquick Spin Columns, Proteinase K, buffers$169.00Add To Cart - Cat No./ID:239035
GelPilot 100 bp Ladder (100)
Ready-to-use DNA marker; 600 µl (100 lanes), 6 fragments: 100–600 bp$156.00Add To Cart - Cat No./ID:13118-400
Glass Beads, 0.1 mm (400 g)
Customize your bead tubes with bulk beads for rapid and reliable biological sample lysis from a wide variety of starting materials.$264.00Add To Cart - Cat No./ID:9002755
TissueRuptor II (120 V, 60 Hz, US)
Handheld rotor–stator homogenizer, 120 V, 60 Hz (for North America), 5 TissueRuptor Disposable Probes$982.00Add To Cart
