RNeasy Maxi Kit
- Fast procedure delivering high-quality total RNA in minutes
- Ready-to-use RNA for high performance in any downstream application
- Consistent RNA yields from large amounts of starting material
- No phenol/chloroform extraction
- No CsCl gradients, no LiCl or ethanol precipitation
The RNeasy Maxi Kit provides fast purification of high-quality RNA from cells, tissues, and yeast using silica-membrane RNeasy spin columns with a binding capacity of 6 mg RNA. Tissue samples can be conveniently stabilized using RNAprotect TissueReagent or Allprotect Tissue Reagent, and efficiently disrupted using a TissueRuptor or TissueLyser system. For smaller samples, the RNeasy Micro Kit (spin-column binding capacity of up to 45 µg RNA), the RNeasy Mini Kit (spin-column binding capacity of 100 µg RNA), and RNeasy Midi Kit (spin-column binding capacity of 1 mg RNA) are also available.
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Cat No./ID:75162 RNeasy Maxi Kit (12) $374.00 12 RNeasy Maxi Spin Columns, Collection Tubes (50 ml), RNase-free Reagents and Buffers |
Product Details
Total RNA purified with the RNeasy Maxi Kit is of high quality and is suitable for many downstream applications (see figure "High-quality RNA from a variety of samples"). Total RNA is easily purified with the RNeasy Maxi Kit from large amounts of starting material including animal or human cells, animal or human tissues, and yeast cells (see table).
| Source | Starting material | Average yield (mg) |
| Animal cells | ||
| LMH | 5 x 108 | 5.7 |
| HeLa | 4 x 108 | 6.0 |
| COS-7 | 1.8 x 108 | 5.8 |
| Lymphocytes (unstimulated) | 5 x 108 | 0.3 |
| Mouse tissue | ||
| Liver | 1 g | 3.6 |
| Lung | 0.5 g | 0.6 |
| Spleen | 1 g | 3.2 |
| Yeast cells | ||
| S. cerevisiae | 1 x 109 | 2.4 |
The RNeasy Maxi Kit delivers efficient purification of total RNA from large amounts of starting material. RNeasy technology simplifies total RNA isolation by combining the stringency of guanidine-isothiocyanate lysis with the speed and purity of silica-membrane purification. Total RNA is easily purified from animal or human cells, animal or human tissues, and yeast. Protocols are included in the kit for cleanup of partially purified RNA, in vitro transcripts, and RNA from enzymatic reactions. RNeasy purified nucleic acids can be used in any molecular assay or other downstream application.
RNA purified with RNeasy technology has A260/280 ratios of 1.9–2.1 (measured in 10 mM Tris·Cl, pH 7.5)and is ideal for use in all applications. Downstream applications include:
- Northern, dot, and slot blotting
- End-point RT-PCR
- Quantitative, real-time RT-PCR
- Array analysis
- Poly A+ RNA selection
Specifications
Features | Specifications |
| Applications | PCR, qPCR, real-time RT-PCR, microarray |
| Elution volume | 800–2400 µl |
| Format | Spin column |
| Main sample type | Tissue, cells |
| Processing | Manual |
| Purification of total RNA, miRNA, poly A+ mRNA, DNA or protein | RNA |
| Sample amount | 150 mg – 1 g |
| Technology | Silica technology |
| Time per run or per prep | 1 hour |
| Yield | Varies |
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