Qiagen/RNeasy Plus 96试剂盒/用于12 x 96总RNA制备：12个RNeasy 96板、12个gDNA消除器96板、洗脱微管CL、盖、S-块、空气带片、无核糖核酸酶试剂和缓冲液/74192
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商品介绍
RNeasy Plus 96 Kit
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For 96-well purification of total RNA from cells using gDNA Eliminator plates
- Integrated removal of genomic DNA
- No need for DNase digestion
- Fast, convenient sample processing
- Reproducible RNA yields from up to 2,000,000 cells
- Highly standardized procedure
The RNeasy Plus 96 Kit provides high-throughput purification of total RNA from cultured cells grown in 96-well plates. The kit combines the use of novel gDNA Eliminator 96 plates with RNeasy 96 plates to ensure purification of RNA that is virtually free of genomic DNA contamination. The fast, standardized purification procedure delivers reproducible yields of high-quality RNA, ensuring reliable results in real-time RT-PCR, microarray analysis, and other downstream applications.For optimal results it is recommended to use this product together with QIAvac96 System.
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Cat No./ID:74192 RNeasy Plus 96 Kit (12) $5,141.00 For 12 x 96 total RNA preps: 12 RNeasy 96 Plates, 12 gDNA Eliminator 96 Plates, Elution Microtubes CL, Caps, S-Blocks, AirPore Tape Sheets, RNase-Free Reagents and Buffers |
The RNeasy Plus 96 Kit is intended for molecular biology applications. This product is not intended for the diagnosis, prevention, or treatment of a disease.
Product Details
Performance
The RNeasy Plus 96 Kit is compatible with a wide range of cell lines, providing purification of high-quality RNA with Agilent RIN values of close to 10 (see figure "High-quality RNA"). Complete genomic DNA removal by the kit (see figure "Effective genomic DNA removal") enables specific transcript detection in highly sensitive applications, such as quantitative, real-time RT-PCR analysis of low-abundance targets. In addition, reliable RNA purification from 102 to 106 cells allows real-time RT-PCR quantification over a wide dynamic range (see figure "Reliable RNA purification").
Principle
Cells are first lysed and homogenized in a highly denaturing guanidine-isothiocyanate–containing buffer, which immediately inactivates RNases to ensure isolation of intact RNA. The lysates are then passed through a gDNA Eliminator 96 plate. This 96-well plate, in combination with the high-salt buffer, selectively and efficiently removes genomic DNA. Ethanol is added to provide appropriate binding conditions for RNA, and the samples are applied to an RNeasy 96 plate. Each well of these specially designed 96-well plates contains a silica membrane that specifically binds RNA from lysed cells.
Procedure
High-throughput RNA purification is achieved through the use of RNeasy 96 plates. Prior to application to theRNeasy 96 plate, cell lysates are first passed through a gDNA Eliminator 96 plate, (see figure "RNeasy Plus 96 procedure"). The gDNA Eliminator 96 plates are conveniently processed using a centrifuge (Centrifuge 4-16). RNA purification using RNeasy 96 plates is manual, and comprises 3 simple steps: bind, wash, and elute. The plates are rapidly and conveniently processed using either a centrifuge (Centrifuge 4-16 and Plate Rotor 2 x 96) or a combination of vacuum (QIAvac 96) and centrifuge. Up to 2 x 106 cells can be used as starting material when processing plates using a centrifuge, while up to 1 x 106 cells can be used when processing plates using a vacuum and centrifuge. The procedure provides an enrichment for mRNA since most RNAs <200 nucleotides (such as rRNAs and tRNAs) are excluded. The RNeasy Plus procedure can be modified to allow the purification of total RNA containing small RNAs, such as miRNA, from cultured cells.
Applications
The purified total RNA, which represents the transcriptome state of the cell, is ideally suited for downstream applications that are sensitive to low amounts of DNA contamination, such as Quantitative real-time RT-PCR. The purified RNA can also be used in other applications.
The ease with which RNA is purified in 96-well format makes the kit well suited for high-throughput gene expression analysis in areas such as:- Drug discovery
- Biomedical research
Specifications
Features | Specifications |
| Applications | End-point RT-PCR, quantitative, real-time RT-PCR |
| Elution volume | 45–140 µl |
| Format | 96-well plate |
| Main sample type | Cultured cells |
| Processing | Manual (centrifugation, vacuum) |
| Purification of total RNA, miRNA, poly A+ mRNA, DNA or protein | RNA |
| Sample amount | 10 to 5 x 10e5 cells |
| Technology | Silica technology |
| Yield | 1.3–3.1 µg (from 1 x 10e5 cells) |
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品牌介绍
QIAGEN是一家专业化致力于生物分子样品制备解决方案的跨国经营企业，总部位于德国。1984年，QIAGEN在德国成立，1996年在美国纽约纳斯达克上市。QIAGEN拥有超过1000项专利和认可证明，在18个国家设立了分公司，代理商服务国超过40个，在全球有超过400000的用户。QIAGEN提供的产品超过500类，包括各种试剂，耗材和自动化纯化工作站。这些产品用于样品采集，稳定，核酸或蛋白的分离，纯化和检测中，不仅广泛的应用于科研领域的各个方面，在生物技术，制药，法医研究，食品安全检测，畜牧业和分子诊断领域也得到了广泛的应用。QIAGEN产品的卓越品质和在应用中的出色表现使得其成为样品处理中标准的代名词。
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