Qiagen/miRNeasy FFPE试剂盒/50 RNeasy MinElute旋转柱、收集管、蛋白酶K、无核糖核酸酶DNA酶I、DNA酶增强缓冲液、无核糖核酸酶缓冲液、无核糖核酸酶水/217504
市场价：
¥9200.00
美元价：
5520.00
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
miRNeasy FFPE Kit
Print
For purification of microRNA and total RNA from formalin-fixed, paraffin-embedded tissue sections
- Effective purification of miRNA and total RNA
- Novel method to overcome formalin crosslinking
- Efficient release of RNA without compromising integrity
- Streamlined protocol providing RNA in just 85 minutes
The miRNeasy FFPE Kit enables purification of total RNA that includes RNA from approximately 18 nucleotides upwards from formalin-fixed, paraffin-embedded (FFPE) tissue sections. The kit provides recovery of usable RNA fragments, including miRNA and other small RNAs, for applications such as quantitative, real-time RT-PCR. Procedures canbe automated on theQIAcube Connect.
- Buy Products
- Product Details
- Specifications
Buy Products
Cat No./ID:217504 miRNeasy FFPE Kit (50) $460.00 50 RNeasy MinElute Spin Columns, Collection Tubes, Proteinase K, RNase-Free DNase I, DNase Booster Buffer, RNase-Free Buffers, RNase-Free Water |
The miRNeasy FFPE Kit is intended for molecular biology applications. This product is not intended for the diagnosis, prevention, or treatment of a disease.
Product Details
Performance
Yields and performanceof total RNA and miRNAwith the miRNeasy FFPE Kit are superior to alternative methods of miRNA purification, such as using phenol-chloroform extraction or a similar kit from another supplier (see figures "Effective real-time RT-PCR quantification", "Efficient RNA purification from FFPE tissues" and Superior yields and performance).
Principle
Fixing tissues with formalin leads to RNA-RNA and RNA-protein crosslinking which impairs RNA performance in downstream applications. The miRNeasy FFPE Kit provides special lysis and incubation conditions to reverse formalin crosslinking of RNA. A specially developed lysis buffer efficiently releases RNA from tissue sections while avoiding further RNA degradation. The lysate is treated with DNase then optimized binding conditions allow purification of all usable RNA from approximately 18 nucleotides upwards.
Procedure
The entire miRNeasy FFPE procedure can be completed in as little as 85 minutes. The optimized lysis buffer allows sample lysis with proteinase K digestion in only 15 minutes without sacrificing RNA yields. After lysis, samples are incubated at 80ºC for 15 minutes to reverse formalin crosslinking. Genomic DNA is then rapidly removed in an optimized DNase step with DNase Booster Buffer, and concentrated RNA is purified using RNeasy MinElute spin columns (see flowchart "miRNeasy FFPE procedure"). Since RNA is eluted in a volume of just 14–30 µl, smaller reaction volumes are possible in downstream applications.
Applications
The miRNeasy FFPE Kit allows purification of miRNA with total RNA for use in a variety of applications, such as quantitative, real-time RT-PCR.
Specifications
Features | Specifications |
| Applications | PCR, qPRC, real-time RT-PCR, microArray |
| Elution volume | 14-30 µl |
| Format | Spin column |
| Main sample type | FFPE tissue samples |
| Processing | Manual (protocol for automated processing on QIAcube available) |
| Purification of total RNA, miRNA, poly A+ mRNA, DNA or protein | miRNA, total RNA |
| Sample amount | up to 4 sections, each with a thickness up to 10 µm and a surface area up to 250mm^2 (automated protocol on QIAcube: up to 2 sections) |
| Technology | Silica technology |
| Yield | Varies |
Product Resources
Customers who bought these products also bought
- Cat No./ID:19093
Deparaffinization Solution (16 ml)
2 x 8 ml Deparaffinization Solution$191.00Add To Cart - Cat No./ID:56404
QIAamp DNA FFPE Tissue Kit (50)
For 50 DNA preps: 50 QIAamp MinElute Columns, Proteinase K, Buffers, Collection Tubes (2 ml)$256.00Add To Cart - Cat No./ID:762331
PAXgene 96 Blood RNA Kit (4)
4 PAXgene 96 RNA Plates, 4 PAXgene 96 Filter Plates, Buffers, Proteinase K, RNase-free DNase Sets, AirPore Tape Sheets, Collection Vessels. To be used in conjunction with PAXgene Blood RNA Tubes.$3,870.00Add To Cart - Cat No./ID:217004
miRNeasy Mini Kit (50)
For 50 preps: 50 RNeasy Mini Spin Columns, Collection Tubes (1.5 ml and 2 ml), QIAzol Lysis Reagent, RNase-Free Reagents and Buffers$390.00Add To Cart - Cat No./ID:79254
RNase-Free DNase Set (50)
1500 Kunitz units RNase-free DNase I, RNase-free Buffer RDD, and RNase-free water for 50 RNA minipreps$113.00Add To Cart - Cat No./ID:217184
miRNeasy Serum/Plasma Kit (50)
For 50 total RNA preps: 50 RNeasy MinElute Spin Columns, Collection Tubes (1.5 ml and 2 ml), QIAzol Lysis Reagent, Ce_miR-39_1 miScript Primer Assay, RNase-free Reagents and Buffers$489.00Add To Cart - Cat No./ID:995652
Filter-Tips, 1000 µl, M48 (1000)
1000 Disposable Filter-Tips for use with the BioRobot M48$502.00Add To Cart - Cat No./ID:12240-50
QIAamp BiOstic Bacteremia DNA Kit (50)
For the isolation of bacterial DNA from cultured blood, fecal and cervical swabs$312.00Add To Cart - Cat No./ID:217204
miRNeasy Serum/Plasma Advanced Kit (50)
For 50 total RNA preps: 50 RNeasy MinElute Spin Columns, Collection Tubes (1.5 ml and 2 ml), RNase-Free Reagents and Buffers$461.00Add To Cart - Cat No./ID:218073
miScript SYBR Green PCR Kit (200)
For 200 reactions: QuantiTect SYBR Green PCR Master Mix, miScript Universal Primer$478.00Add To Cart - Cat No./ID:939018
Deparaffinization Solution(50 ml)
1 x 50ml Deparaffinization Solution$349.00Add To Cart - Cat No./ID:32149
QIAexpress Type IV Kit
5 μg each: pQE-30, pQE-31, pQE-32 (N-terminal 6xHis); 10 ml Ni-NTA Agarose$741.00Add To Cart - Cat No./ID:219610
miRNeasy Serum/Plasma Spike-In Control
10 pmol lyophilized C. elegans miR-39 miRNA mimic$51.30Add To Cart - Cat No./ID:218075
miScript SYBR Green PCR Kit (1000)
For 1000 reactions: QuantiTect SYBR Green PCR Master Mix, miScript Universal Primer$2,068.00Add To Cart - Cat No./ID:218161
miScript II RT Kit (50)
For 50 cDNA synthesis reactions: miScript Reverse Transcriptase Mix, 10x miScript Nucleics Mix, 5x miScript HiSpec Buffer, 5x miScript HiFlex Buffer, RNase-Free Water$522.00Add To Cart - Cat No./ID:180134
GeneRead DNA FFPE Kit
For 50 preps: QIAamp MinElute Columns, Collection Tubes, Deparaffinization Solution, Uracil-N-Glycosylase, RNase-Free Water, RNase A, and Buffers$328.00Add To Cart
品牌介绍
QIAGEN是一家专业化致力于生物分子样品制备解决方案的跨国经营企业，总部位于德国。1984年，QIAGEN在德国成立，1996年在美国纽约纳斯达克上市。QIAGEN拥有超过1000项专利和认可证明，在18个国家设立了分公司，代理商服务国超过40个，在全球有超过400000的用户。QIAGEN提供的产品超过500类，包括各种试剂，耗材和自动化纯化工作站。这些产品用于样品采集，稳定，核酸或蛋白的分离，纯化和检测中，不仅广泛的应用于科研领域的各个方面，在生物技术，制药，法医研究，食品安全检测，畜牧业和分子诊断领域也得到了广泛的应用。QIAGEN产品的卓越品质和在应用中的出色表现使得其成为样品处理中标准的代名词。
联络我们
