RNeasy 96 Universal Tissue Kit
For 96-well purification of total RNA from any tissue
- High yields of total RNA from all tissues
- Streamlined QIAzol lysis and RNeasy 96 purification
- Pure, high-quality RNA without phenol contamination
- High-performance RNA for all downstream applications
The RNeasy 96 Universal Tissue Kit enables total RNA purification in 96-well format from any tissue. Tissues are efficiently lysed and homogenized in QIAzol Lysis Reagent, and high-quality RNA is purified using silica-membrane RNeasy 96 plates. Manual purification is carried out using the QIAGEN 96-Well-Plate Centrifugation System and, optionally, the QIAvac 96 vacuum manifold. Automated purification using the related RNeasy 96 Universal Tissue 8000 Kit is performed on the BioRobot Universal System. Tissue samples can be conveniently stabilized using Allprotect Tissue Reagent, and efficiently disrupted using the TissueLyser II. Nonfatty tissues can be stabilized using the RNAprotect Tissue Reagent.
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Cat No./ID:74881 RNeasy 96 Universal Tissue Kit (4) $2,086.00 For 4 x 96 total RNA preps: 4 RNeasy 96 Plates, Collection Microtubes, Elution Microtubes CL, Caps, S-Blocks, AirPore Tape Sheets, QIAzol Lysis Reagent, RNase-Free Reagents and Buffers |
Product Details
Compared with standard silica-membrane procedures, the RNeasy 96 Universal Tissue Kit provides higher yields of total RNA for all tissue types (see table).
| Tissue | RNA yield* (µg per 10 mg of tissue) |
|---|---|
| Kidney | 5–40 |
| Liver | 15–80 |
| Lung | 5–15 |
| Heart | 5–25 |
| Muscle | 5–35 |
| Brain | 5–20 |
| Adipose tissue | 0.5–2.5 |
| Spleen | 15–100 |
| Intestine | 10–60 |
| Skin | 2–5 |
High-quality RNA can be isolated from tissues preserved either by freezing or by stabilization with RNAprotectat room temperature(see figure "High-quality total RNA from rat liver and muscle tissue"). Intact RNA purifiedfrom difficult-to-lyse fibrous and fatty tissues is suitable for downstream applications (see figures"High-quality RNA fromskin /adipose/liver/intestinetissue" and "Real-time analysis of high-quality RNA from rat brain").
The RNeasy 96 Universal Tissue procedure enables high-throughput purification of RNA from any animal or human tissue sample. Compared with standard silica-membrane procedures, the RNeasy 96 Universal Tissue Kit provides higher yields of total RNA for all tissue types. The RNeasy procedure enriches for RNA >200 bases long, providing a yield of total RNA that does not include 5.8S rRNA, tRNA, and other low-molecular weight RNAs, which make up 15–20% of total cellular RNA.
The combination of QIAzol and RNeasy technologies results in highly pure RNA without phenol carryover. RNA purified using the RNeasy 96 Universal Tissue Kit is suitable for all downstream applications, including real-time RT-PCR and array analysis.
Specifications
Features | Specifications |
| Applications | PCR, qPCR, real-time PCR |
| Elution volume | 45–70 µl |
| Format | 96-well plate |
| Main sample type | Tissue samples |
| Processing | Manual (centrifugation or vacuum) |
| Purification of total RNA, miRNA, poly A+ mRNA, DNA or protein | RNA |
| Sample amount | 25–100 mg |
| Technology | Silica technology |
| Yield | Varies |
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